Detailed information NTG0426260

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: G6R31_RS01530
• Genome accession: NZ_CP049357
• Coordinates: 293411..296074 (+)
• Length: 887 a.a.
• NCBI ID: WP_017870162.1
• Uniprot ID: A0AAV4KA40
• Organism: Deinococcus wulumuqiensis R12
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 288411..301074

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G6R31_RS01495 (G6R31_01495)	-	288680..289198 (+)	519	WP_081608229.1	winged helix-turn-helix domain-containing protein	-
G6R31_RS01505 (G6R31_01505)	-	289893..290243 (-)	351	WP_017870157.1	helix-turn-helix domain-containing protein	-
G6R31_RS01510 (G6R31_01510)	-	290333..290971 (+)	639	WP_017870158.1	nitroreductase family protein	-
G6R31_RS01515 (G6R31_01515)	-	291046..291840 (-)	795	WP_017870159.1	enoyl-ACP reductase	-
G6R31_RS01520 (G6R31_01520)	pgeF	291921..292670 (+)	750	WP_017870160.1	peptidoglycan editing factor PgeF	-
G6R31_RS01525 (G6R31_01525)	-	292667..293176 (+)	510	WP_017870161.1	YqeG family HAD IIIA-type phosphatase	-
G6R31_RS01530 (G6R31_01530)	pilB	293411..296074 (+)	2664	WP_017870162.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
G6R31_RS01535 (G6R31_01535)	pilT	296113..297375 (+)	1263	WP_114671147.1	type IV pilus twitching motility protein PilT	Machinery gene
G6R31_RS01540 (G6R31_01540)	-	297487..297999 (-)	513	WP_017870164.1	hypothetical protein	-
G6R31_RS01545 (G6R31_01545)	-	298256..300898 (+)	2643	WP_017870165.1	S8 family serine peptidase	-

Sequence

Protein

• Length: 887 a.a.
• Molecular weight: 97154.99 Da
• Isoelectric Point: 4.8612

>NTDB_id=426260 G6R31_RS01530 WP_017870162.1 293411..296074(+) (pilB) [Deinococcus wulumuqiensis R12]
MALSIGDRRLGAILLDQGYLGDNDLQRALERHAEVGGRLADVLIDSGMVGERRIARAIEEALGIPLVNLLAVQPDPAALR
SIRPQTALNLQAFPFALEGDRLRVALVDPLSSFSIETLEDDSGLDIEPYQALREEVLWALATHYPELGLDIVVPSGVSET
GRAGGKLGDRLIMHGYITDAQLQVALDAQQQTGEALGATLISQRAITEDQLYEVLAEQEGTTFLPNPSGFHPGEEVLGSM
LRADALRLLAVPVDETEQGVTVLTSDPRKRPDIGALIGRPVQLMLTRPRDIERLIEQFYPQRGRLGEQLVQEGALSRDQL
REALQVQAREGKVKPLGEIITELGFASPDEVDSALQKQNVGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEFLDPIQNPP
DPKVALMIPESTARRYVVVPVRLQGNSLVVAMKDPRNVFALDDLKLITGKDILPAVMAEKDIIRLIERYFGEKGFEKLNK
ELAERNKTQQSQEVDLSVADESAIVQVVDSIIREAALQDASDIHIETTDDGVKVRYRIDGALRDQNSFPKGAAQQIMARL
KIMGHLDIAERRIPQDGRIRFKKGSIDLDLRLSTLPTVYGEKAVMRLLQKASNIPELEQLGFSEYNYARYTEIIERPNGI
FLVTGPTGSGKSFTCFSTLKRIAKPEKNTTTIEDPIEYEVPGIVQSQVNNTTGMTFARALRAFLRQDPDIIFVGEIRDTE
TARIAVEAALTGHMVLATLHTNDAPGAVTRLEEMGVENFNISASVMGVLAQRLVRRVCSECKQPTNADPEVLRRLGIGER
EIRGANLVRGAGCPRCGGTGYKGRMGIHELMVIDDSLRRAIGAGRPAAEIRDIALNESGLRSLRQDGIEKALQGLTTLEE
VLAATAN

Nucleotide

• Length: 2664 bp

>NTDB_id=426260 G6R31_RS01530 WP_017870162.1 293411..296074(+) (pilB) [Deinococcus wulumuqiensis R12]
TTGGCTCTTTCGATCGGTGACCGCCGTCTGGGCGCGATTTTGCTGGACCAGGGGTATCTGGGCGACAACGACTTGCAGCG
GGCGCTGGAGCGGCACGCCGAGGTGGGGGGGCGGCTGGCCGACGTCCTTATCGACTCGGGCATGGTGGGCGAAAGACGCA
TTGCCCGCGCCATCGAGGAGGCGCTGGGCATCCCGCTGGTCAACCTGCTGGCGGTGCAGCCCGACCCGGCGGCGCTGCGC
TCGATTCGGCCCCAGACGGCCCTCAACCTGCAAGCGTTTCCCTTCGCACTGGAGGGCGACCGGCTGCGGGTGGCGCTGGT
GGACCCGCTGTCGAGCTTTTCTATCGAGACCCTGGAGGACGACAGCGGCCTCGATATCGAACCGTATCAGGCGCTGCGCG
AGGAAGTGCTGTGGGCGCTCGCGACGCACTATCCCGAACTGGGCCTCGACATCGTGGTGCCCAGCGGCGTGTCGGAAACG
GGGCGCGCCGGGGGCAAGCTCGGTGACCGGCTGATCATGCACGGCTACATCACCGACGCGCAGCTTCAGGTGGCGCTCGA
CGCGCAGCAGCAGACCGGCGAGGCGCTCGGGGCCACCCTGATCTCGCAGCGGGCGATTACCGAAGACCAGCTCTACGAAG
TGCTGGCCGAGCAGGAAGGCACCACCTTCCTTCCCAACCCCAGCGGCTTTCACCCTGGCGAGGAAGTGCTCGGCAGTATG
CTCCGCGCCGACGCCTTGCGCCTCCTGGCCGTGCCGGTGGACGAAACCGAGCAGGGCGTGACGGTGCTCACCAGCGACCC
GCGCAAGCGCCCCGACATCGGCGCGCTGATCGGGCGCCCGGTGCAGCTGATGCTCACGCGCCCGCGCGACATCGAGCGAT
TGATCGAGCAGTTCTACCCCCAGCGCGGGCGCCTGGGCGAACAGCTCGTGCAGGAAGGGGCGCTGTCACGCGATCAACTT
CGTGAGGCGCTTCAGGTCCAGGCCCGCGAGGGCAAGGTCAAGCCGCTGGGCGAGATCATCACCGAACTGGGCTTTGCCAG
CCCCGACGAGGTGGATTCGGCGCTGCAAAAGCAGAACGTGGGCGGGGGCCGCCTCGAAGACACCCTGGTGCAGTCGGGCA
AGCTCAGCCCCGAAATGCTCGCCCGCTCGCTGGCCGCGCAGCTCGGCTACGAGTTTCTCGACCCCATCCAGAACCCGCCG
GACCCCAAGGTCGCGCTGATGATTCCCGAGTCCACCGCCCGGCGGTACGTGGTGGTTCCGGTGCGTCTCCAGGGCAACTC
GCTCGTCGTCGCCATGAAGGACCCGCGCAACGTGTTTGCGCTCGACGACCTCAAGCTGATCACCGGCAAGGACATCCTGC
CCGCCGTGATGGCGGAAAAGGACATCATCCGCCTGATCGAGCGCTACTTCGGGGAAAAGGGCTTCGAGAAGCTCAACAAG
GAACTCGCCGAGCGCAACAAGACCCAGCAGTCGCAGGAAGTCGACCTCTCGGTGGCCGACGAGAGCGCCATCGTGCAGGT
GGTGGACTCGATCATCCGCGAGGCCGCGCTGCAGGACGCGTCGGACATCCACATCGAAACCACCGACGACGGCGTCAAGG
TGCGCTACCGCATCGACGGAGCGCTGCGCGACCAGAACTCCTTTCCCAAGGGCGCGGCGCAGCAGATTATGGCCCGCCTC
AAAATCATGGGGCACCTCGACATCGCCGAGCGGCGCATTCCCCAGGACGGGCGCATCCGCTTCAAAAAGGGCAGCATCGA
CCTCGACCTGCGTCTCTCGACCCTGCCCACCGTGTACGGCGAAAAGGCCGTGATGCGTCTGCTGCAAAAGGCGAGCAACA
TCCCCGAACTCGAGCAGCTTGGATTCTCCGAGTACAACTACGCCCGCTACACCGAAATCATCGAGCGGCCCAACGGGATT
TTTCTGGTCACCGGGCCGACGGGGTCGGGCAAGTCGTTCACCTGCTTTTCGACCCTCAAGCGCATCGCCAAGCCCGAGAA
AAACACCACGACCATCGAAGACCCCATCGAGTACGAGGTGCCGGGCATCGTGCAGTCGCAGGTGAACAACACCACCGGCA
TGACGTTCGCCCGCGCGCTGCGCGCCTTTCTCCGTCAGGACCCCGACATCATCTTCGTGGGCGAGATCCGGGATACTGAA
ACCGCCAGGATTGCCGTGGAAGCCGCGCTCACCGGCCACATGGTGCTGGCGACGCTGCACACCAACGACGCGCCGGGCGC
CGTGACCCGACTGGAAGAAATGGGCGTCGAGAACTTCAACATCTCGGCGTCGGTCATGGGCGTGCTCGCGCAGCGGCTGG
TGCGCCGGGTGTGCAGCGAGTGCAAGCAGCCCACCAACGCCGACCCCGAAGTGCTGCGGCGCCTGGGCATCGGCGAGCGC
GAGATTCGCGGCGCCAACCTGGTGCGCGGGGCCGGGTGTCCGCGTTGCGGCGGCACCGGCTACAAGGGCCGCATGGGGAT
TCACGAGCTGATGGTGATTGACGACTCGCTGCGCCGCGCCATCGGGGCCGGGCGCCCCGCCGCCGAGATTCGTGACATCG
CGCTCAACGAAAGCGGCCTGCGCAGCCTGCGTCAGGACGGCATCGAGAAAGCCTTGCAGGGCCTGACCACCCTCGAAGAA
GTGCTGGCCGCCACCGCCAACTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-472)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-145)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (200-303)

• Type II/IV secretion system protein (T2SSE) (511-776)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	95.716	100	0.957
pilF	Thermus thermophilus HB27	56.678	100	0.569