Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CE10_RS00585 Genome accession   NC_017646
Coordinates   125786..126226 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli O7:K1 str. CE10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 120786..131226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CE10_RS00565 (CE10_0104) coaE 121276..121896 (-) 621 WP_001269524.1 dephospho-CoA kinase -
  CE10_RS30625 - 121921..121965 (+) 45 WP_120795372.1 protein YacM -
  CE10_RS00570 (CE10_0105) guaC 122121..123164 (+) 1044 WP_001217337.1 GMP reductase -
  CE10_RS00575 (CE10_0106) hofC 123199..124401 (-) 1203 WP_000157220.1 protein transport protein HofC -
  CE10_RS00580 (CE10_0107) pilB 124391..125776 (-) 1386 WP_001025161.1 type II secretion system protein GspE Machinery gene
  CE10_RS00585 (CE10_0108) pilA 125786..126226 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  CE10_RS00595 (CE10_0109) nadC 126429..127322 (-) 894 WP_001135167.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CE10_RS00600 (CE10_0110) ampD 127410..127961 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CE10_RS00605 (CE10_0111) ampE 127958..128812 (+) 855 WP_000171985.1 beta-lactamase regulator AmpE -
  CE10_RS00610 (CE10_0112) aroP 128855..130228 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=41851 CE10_RS00585 WP_000360914.1 125786..126226(-) (pilA) [Escherichia coli O7:K1 str. CE10]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=41851 CE10_RS00585 WP_000360914.1 125786..126226(-) (pilA) [Escherichia coli O7:K1 str. CE10]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment