Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N026_RS03765 Genome accession   NZ_CP047267
Coordinates   830995..831423 (+) Length   142 a.a.
NCBI ID   WP_024643089.1    Uniprot ID   A0AAJ4B1G3
Organism   Pseudomonas syringae UB303     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 825995..836423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N026_RS03740 (N026_03740) yacG 826158..826367 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  N026_RS03745 (N026_03745) coaE 826364..826987 (-) 624 WP_024661082.1 dephospho-CoA kinase -
  N026_RS03750 (N026_03750) pilD 826984..827856 (-) 873 WP_024661083.1 prepilin peptidase Machinery gene
  N026_RS03755 (N026_03755) pilC 827858..829075 (-) 1218 WP_024643091.1 type II secretion system F family protein Machinery gene
  N026_RS03760 (N026_03760) pilB 829078..830772 (-) 1695 WP_024643090.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N026_RS03765 (N026_03765) pilA 830995..831423 (+) 429 WP_024643089.1 pilin Machinery gene
  N026_RS03770 (N026_03770) - 831638..831835 (-) 198 WP_080267182.1 DUF1523 family protein -
  N026_RS03775 (N026_03775) - 832042..832734 (+) 693 WP_032626650.1 DUF433 domain-containing protein -
  N026_RS03780 (N026_03780) - 832782..833327 (-) 546 WP_004415097.1 DUF1523 family protein -
  N026_RS03785 (N026_03785) - 833447..834445 (+) 999 WP_011266638.1 ATP-grasp domain-containing protein -
  N026_RS03790 (N026_03790) - 834501..835640 (+) 1140 WP_024661085.1 trypsin-like peptidase domain-containing protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14077.35 Da        Isoelectric Point: 8.1130

>NTDB_id=411951 N026_RS03765 WP_024643089.1 830995..831423(+) (pilA) [Pseudomonas syringae UB303]
MQKQKGFTLIELMIVVAIIGILAAVAIPAYQNYVTKAQVTSALAEIEPGRTMAETKINEGVTTTLTAAADVGLAASTRCP
TLGISVASSGIASIACTMAGNPGVATKTVTLSRTAAGAWSCATTVAADFAPKGCTVTAAAGG

Nucleotide


Download         Length: 429 bp        

>NTDB_id=411951 N026_RS03765 WP_024643089.1 830995..831423(+) (pilA) [Pseudomonas syringae UB303]
ATGCAAAAACAAAAAGGTTTTACGTTGATTGAATTGATGATCGTAGTCGCGATCATCGGGATTTTGGCTGCAGTGGCTAT
CCCTGCTTATCAGAACTACGTTACGAAAGCTCAGGTAACCTCTGCTCTGGCTGAAATTGAGCCTGGCAGGACTATGGCTG
AAACAAAAATTAATGAAGGTGTTACTACTACATTAACCGCGGCTGCGGACGTTGGGCTTGCGGCTTCAACCCGCTGCCCG
ACGCTTGGTATTTCAGTGGCCAGCAGTGGTATTGCTTCGATCGCCTGTACGATGGCGGGTAATCCAGGTGTTGCAACCAA
GACTGTTACACTCTCCCGGACAGCCGCTGGCGCTTGGTCTTGCGCTACTACGGTAGCCGCGGATTTCGCGCCTAAAGGAT
GCACTGTTACCGCTGCTGCCGGGGGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

52.518

97.887

0.514
  pilA/pilAI Pseudomonas stutzeri DSM 10701

50.725

97.183

0.493
  pilA Pseudomonas aeruginosa PAK

42.484

100

0.458
  pilA Vibrio cholerae C6706

43.836

100

0.451
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.836

100

0.451
  pilA Vibrio cholerae strain A1552

43.836

100

0.451
  pilA Vibrio parahaemolyticus RIMD 2210633

43.448

100

0.444
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.776

94.366

0.423
  comP Acinetobacter baylyi ADP1

37.748

100

0.401
  pilA2 Legionella pneumophila strain ERS1305867

41.481

95.07

0.394
  pilA2 Legionella pneumophila str. Paris

41.481

95.07

0.394

Multiple sequence alignment