Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N026_RS03760 Genome accession   NZ_CP047267
Coordinates   829078..830772 (-) Length   564 a.a.
NCBI ID   WP_024643090.1    Uniprot ID   A0AAJ4AW90
Organism   Pseudomonas syringae UB303     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 824078..835772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N026_RS03735 (N026_03735) - 825570..826031 (+) 462 WP_024661081.1 hypothetical protein -
  N026_RS03740 (N026_03740) yacG 826158..826367 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  N026_RS03745 (N026_03745) coaE 826364..826987 (-) 624 WP_024661082.1 dephospho-CoA kinase -
  N026_RS03750 (N026_03750) pilD 826984..827856 (-) 873 WP_024661083.1 prepilin peptidase Machinery gene
  N026_RS03755 (N026_03755) pilC 827858..829075 (-) 1218 WP_024643091.1 type II secretion system F family protein Machinery gene
  N026_RS03760 (N026_03760) pilB 829078..830772 (-) 1695 WP_024643090.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N026_RS03765 (N026_03765) pilA 830995..831423 (+) 429 WP_024643089.1 pilin Machinery gene
  N026_RS03770 (N026_03770) - 831638..831835 (-) 198 WP_080267182.1 DUF1523 family protein -
  N026_RS03775 (N026_03775) - 832042..832734 (+) 693 WP_032626650.1 DUF433 domain-containing protein -
  N026_RS03780 (N026_03780) - 832782..833327 (-) 546 WP_004415097.1 DUF1523 family protein -
  N026_RS03785 (N026_03785) - 833447..834445 (+) 999 WP_011266638.1 ATP-grasp domain-containing protein -
  N026_RS03790 (N026_03790) - 834501..835640 (+) 1140 WP_024661085.1 trypsin-like peptidase domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62368.67 Da        Isoelectric Point: 6.3321

>NTDB_id=411950 N026_RS03760 WP_024643090.1 829078..830772(-) (pilB) [Pseudomonas syringae UB303]
MTDAVLTGLAKQLVLAELLTETAAQQAYQQARRDKISLVSYLVQHKLVKSLTLAELASDQFGVPFMDLASVDKDSQPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTTAIDRFFDTDSGMGSLADIDLGLD
IEPVDGKETSLAAQDDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVAKPPIHLASRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKSLYMEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKEIDIPRETLIREGFPESA
IGTFKIYGPVGCEHCNGGYRGRIGIYEVVKKTPDLERIIMEEGNSLEISRQMRKDGFNDLRTSGLLKAMQGVTSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=411950 N026_RS03760 WP_024643090.1 829078..830772(-) (pilB) [Pseudomonas syringae UB303]
ATGACTGATGCTGTCCTTACTGGCTTGGCCAAACAACTGGTTTTAGCCGAGCTACTTACTGAAACGGCTGCGCAGCAAGC
GTACCAACAAGCGCGTCGTGACAAGATTTCGCTGGTCAGTTATTTGGTACAGCATAAGCTGGTCAAAAGCCTGACGCTGG
CAGAGTTGGCTTCCGATCAGTTCGGGGTGCCATTTATGGACCTTGCCAGCGTAGACAAAGACAGTCAGCCAAAAGGGCTG
GTTAGTGAAAAGCTTGTTCGGCAACACCACGCGCTGCCATTGTGGCGGCGTGGCAACAAGCTATTCATAGGAATATCTGA
TCCGACCAATCATCAAGCGGTCACTGACATCCAATTCAGTACAGGCTTGAATACCGAGGCGATTCTTGTCGAGGATGACA
AGCTCACAACGGCAATAGATCGCTTTTTTGACACTGACAGCGGTATGGGCAGTCTGGCGGACATTGATCTGGGCTTGGAC
ATCGAGCCAGTCGACGGTAAGGAAACGTCGCTGGCAGCTCAGGATGACGCTGATGACGCCCCGGTAGTGCGCTTTGTTAA
CAAGATGCTGATGGATGCCATCAGGCTTGGCTCATCAGATCTACATTTTGAACCATACGAAAAAATATTCCGTGTGCGCC
TGCGTACTGATGGGATTTTGCATGAAGTTGCAAAACCGCCCATACATTTAGCAAGCCGGATAGCTGCGCGCCTGAAAGTG
ATGGCCAGCCTGGACATTTCCGAAAGGCGCAAACCTCAGGACGGGCGGGTCAAGCTGAGGGTGTCGAAAACCAAAGCTAT
CGATTTCCGTATGAATACCTTGCCTACCCTATGGGGCGAGAAGATTGTCATGCGGATTTTAGATCCAACCAGCGCGCAGA
TGGGTATCGATGCCTTAGGCTACGAGCCGGAGCAAAAGTCGCTCTACATGGAAGCGTTGAAGCAGCCGCAGGGGATGATC
CTGGTAACTGGCCCGACCGGTTCCGGCAAGACAGTTTCTCTCTATACGGGTTTAAACATTCTCAACACGGTGGATATCAA
CATTTCCACGGCCGAAGACCCGGTGGAAATCAACCTGGAAGGGATAAATCAGGTCAATGTAAACCCGCGTCAGGGCTTGG
ATTTCTCTCAAGCACTACGTGCCTTCTTGCGCCAAGATCCTGATGTAATCATGGTTGGTGAGATTCGCGATCTGGAGACT
GCCGAGATTGCAATCAAGGCATCGCAGACCGGTCACATGGTGTTATCAACGCTGCATACAAACAGCGCGGCAGAAACCTT
GACGCGTCTCCACCATATGGGTGTCGCAGCGTTCAATATTGCTACCGCGATCAACCTGATCATCGCTCAGCGCTTGGCGC
GCAAGCTGTGCAGCCATTGCAAGAAGGAAATTGATATACCTCGCGAAACACTGATCCGAGAAGGATTCCCAGAGTCGGCT
ATTGGCACCTTCAAGATTTATGGACCCGTAGGTTGCGAACACTGCAATGGCGGATATAGAGGCCGTATCGGTATTTACGA
AGTGGTCAAAAAGACGCCTGATCTCGAACGTATCATCATGGAGGAAGGTAACTCGCTGGAGATTTCCAGACAAATGCGCA
AGGACGGATTCAATGATTTACGCACATCAGGGCTACTGAAAGCTATGCAAGGGGTTACCAGTCTCGAAGAAGTCAACCGC
GTGACAAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.268

100

0.58
  pilB Acinetobacter baylyi ADP1

56.89

100

0.571
  pilB Legionella pneumophila strain ERS1305867

54.417

100

0.546
  pilB Vibrio cholerae strain A1552

50.089

99.468

0.498
  pilB Vibrio campbellii strain DS40M4

49.201

99.823

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

50.09

98.05

0.491
  pilF Neisseria gonorrhoeae MS11

48.128

99.468

0.479
  pilF Thermus thermophilus HB27

38.693

100

0.388

Multiple sequence alignment