Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   N026_RS03750 Genome accession   NZ_CP047267
Coordinates   826984..827856 (-) Length   290 a.a.
NCBI ID   WP_024661083.1    Uniprot ID   -
Organism   Pseudomonas syringae UB303     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 821984..832856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N026_RS03735 (N026_03735) - 825570..826031 (+) 462 WP_024661081.1 hypothetical protein -
  N026_RS03740 (N026_03740) yacG 826158..826367 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  N026_RS03745 (N026_03745) coaE 826364..826987 (-) 624 WP_024661082.1 dephospho-CoA kinase -
  N026_RS03750 (N026_03750) pilD 826984..827856 (-) 873 WP_024661083.1 prepilin peptidase Machinery gene
  N026_RS03755 (N026_03755) pilC 827858..829075 (-) 1218 WP_024643091.1 type II secretion system F family protein Machinery gene
  N026_RS03760 (N026_03760) pilB 829078..830772 (-) 1695 WP_024643090.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N026_RS03765 (N026_03765) pilA 830995..831423 (+) 429 WP_024643089.1 pilin Machinery gene
  N026_RS03770 (N026_03770) - 831638..831835 (-) 198 WP_080267182.1 DUF1523 family protein -
  N026_RS03775 (N026_03775) - 832042..832734 (+) 693 WP_032626650.1 DUF433 domain-containing protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 32039.21 Da        Isoelectric Point: 8.3734

>NTDB_id=411948 N026_RS03750 WP_024661083.1 826984..827856(-) (pilD) [Pseudomonas syringae UB303]
MPLLDLLASSPLAFVTTCCILGLIIGSFLNVVVYRLPIMMERDWKAQSRELLGLPAEPDQPVFNLNRPRSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCSVLSAYVAWHFGFGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
ALLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide


Download         Length: 873 bp        

>NTDB_id=411948 N026_RS03750 WP_024661083.1 826984..827856(-) (pilD) [Pseudomonas syringae UB303]
ATGCCCCTCCTCGACCTCCTGGCCAGCTCCCCACTGGCCTTCGTCACCACCTGCTGCATCCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTGGTCGTCTATCGCCTGCCAATAATGATGGAGCGCGACTGGAAAGCCCAATCCCGTGAACTGCTCGGCC
TGCCCGCCGAACCCGATCAGCCCGTCTTCAACCTAAACCGCCCCCGTTCAAGCTGCCCGCATTGCGCACACAAAATCCGC
CCATGGGAAAATCTGCCGGTGATCAGCTATCTGCTGCTGCGCGGTAAATGTTCGCAGTGCAAAGCGCCGATCAGCAAACG
CTACCCACTGGTCGAGCTGACCTGCTCAGTGTTGTCGGCCTACGTGGCTTGGCATTTCGGCTTTGGCTGGCAGGCGGCGG
CAATGTTGGTACTGAGCTGGGGGCTGCTGGCGATGAGTCTGATCGATGCTGATCACCAGTTGTTACCTGATTCTCTGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTCAACGCTTTCGGGCTGTTCACGTCGCTGAATGATGCGCTATGGGGTGCAGT
GGCCGGCTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTGGGCGCGTGGGGCGGCTGGCAGATTTTGCCGCTGACCATCCTGTTATCGTCGCTGGTGGGC
GCGCTGCTCGGGGTGATCATGATGCGTGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTATGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(136-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

53.004

97.586

0.517
  pilD Vibrio campbellii strain DS40M4

51.943

97.586

0.507
  pilD Acinetobacter baumannii D1279779

52.555

94.483

0.497
  pilD Acinetobacter nosocomialis M2

51.825

94.483

0.49
  pilD Neisseria gonorrhoeae MS11

51.119

92.414

0.472

Multiple sequence alignment