Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GPY13_RS02510 Genome accession   NZ_CP046823
Coordinates   518290..519396 (+) Length   368 a.a.
NCBI ID   WP_000243866.1    Uniprot ID   A0A2J9V0V4
Organism   Vibrio mimicus strain 06-2455     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 513290..524396
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY13_RS02475 - 513508..514110 (-) 603 WP_000725003.1 XTP/dITP diphosphatase -
  GPY13_RS02480 - 514196..514627 (-) 432 WP_001233589.1 DUF4426 domain-containing protein -
  GPY13_RS02485 yggU 514721..515014 (-) 294 WP_000741913.1 DUF167 family protein YggU -
  GPY13_RS02490 - 515014..515571 (-) 558 WP_005509546.1 YggT family protein -
  GPY13_RS02495 proC 515629..516447 (-) 819 WP_000437954.1 pyrroline-5-carboxylate reductase -
  GPY13_RS02500 - 516502..517212 (-) 711 WP_001256643.1 YggS family pyridoxal phosphate-dependent enzyme -
  GPY13_RS02505 pilT 517238..518275 (+) 1038 WP_000350205.1 type IV pilus twitching motility protein PilT Machinery gene
  GPY13_RS02510 pilU 518290..519396 (+) 1107 WP_000243866.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GPY13_RS02515 - 519512..520315 (+) 804 WP_080561465.1 helix-turn-helix transcriptional regulator -
  GPY13_RS02520 tyrS 520424..521704 (+) 1281 WP_001088105.1 tyrosine--tRNA ligase -
  GPY13_RS02525 ruvX 521744..522166 (-) 423 WP_000091871.1 Holliday junction resolvase RuvX -
  GPY13_RS02530 - 522248..522811 (-) 564 WP_001054774.1 YqgE/AlgH family protein -
  GPY13_RS02535 gshB 522847..523803 (-) 957 WP_000593303.1 glutathione synthase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41277.38 Da        Isoelectric Point: 7.3350

>NTDB_id=408441 GPY13_RS02510 WP_000243866.1 518290..519396(+) (pilU) [Vibrio mimicus strain 06-2455]
MVLNQYLDGMLSHKASDLYITVGAPILYRVDGELRAQGEVLSLADVTTLLHAMMDDARQAEFKQTREANFAVVRESGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPAVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMQYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIGQQLLRDKNGKGRHGVFEVLLNSPRVADLIRRGDLHELKTTMARSKEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDNYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=408441 GPY13_RS02510 WP_000243866.1 518290..519396(+) (pilU) [Vibrio mimicus strain 06-2455]
ATGGTGTTGAATCAATATCTGGATGGGATGCTGAGCCATAAAGCATCGGATCTTTACATCACTGTTGGTGCGCCGATTTT
ATATCGGGTCGATGGTGAATTGCGCGCGCAAGGTGAAGTGCTCAGTTTGGCGGATGTGACGACCTTACTGCATGCGATGA
TGGATGACGCGAGACAAGCGGAATTTAAGCAGACGCGTGAAGCGAACTTTGCTGTAGTACGTGAAAGTGGACGTTTCCGG
GTTAGTGCGTTTTTCCAACGTGAATTGCCGGGAGCGGTGATCCGCCGGATTGAAACGCGTATTCCCACCTTTGAAGAGCT
GAAATTACCCGCGGTACTGCAAAATTTAGCGATCGCGAAACGCGGTTTAGTCCTAGTAGTAGGTGCTACGGGGTCAGGTA
AATCGACCACTATGGCGGCAATGACGGGGTATCGTAACCAACACCGTACTGGACATATCTTGACGGTCGAAGATCCGATT
GAGTTTGTGCATGAACATAAGCGTTGTATCGTCACCCAACGAGAAGTCGGCTTAGATACTGAAAGCTATGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCACCGGATATGATTTTGATTGGTGAAATTCGCAGCCGAGAAACCATGCAATACGCGATGA
CCTTCGCGGAAACGGGACATTTGTGTATGGCCACACTGCACGCCAACAACGCCAACCAAGCTTTAGAGCGGATTTTGCAT
TTAGTGCCGAAAGAACAGCGTGAGCAGTTCTTGCTCGACTTGTCACTCAACCTAAAAGGGGTGATTGGCCAGCAACTGTT
GCGCGATAAAAACGGCAAAGGGCGACATGGGGTGTTTGAAGTTCTGCTCAATAGCCCACGAGTCGCGGATTTGATCCGCC
GTGGCGATCTGCATGAGCTTAAAACGACGATGGCGCGTTCCAAAGAAGTAGGCATGCAGACTTTCGACCAAGCTTTGTAT
CAACTGGTGGTGGACGACAAAATCAGCGAGCAAGATGCCCTGCACAGTGCGGATTCAGCCAACGATCTGCGTTTGATGCT
TAAAACCAAGCGCGGTGACAACTACGGCAGTGGTTCTTTGCAGAATGTAAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2J9V0V4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

96.196

100

0.962
  pilU Pseudomonas stutzeri DSM 10701

58

95.109

0.552
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.806

84.239

0.386
  pilT Acinetobacter baumannii D1279779

41.003

92.12

0.378
  pilT Acinetobacter baumannii strain A118

41.003

92.12

0.378
  pilT Acinetobacter nosocomialis M2

41.003

92.12

0.378
  pilT Vibrio cholerae strain A1552

41.018

90.761

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.761

0.372
  pilT Acinetobacter baylyi ADP1

41.846

88.315

0.37
  pilT Pseudomonas aeruginosa PAK

41.538

88.315

0.367
  pilT Pseudomonas stutzeri DSM 10701

40.923

88.315

0.361

Multiple sequence alignment