Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GJB62_RS00575 Genome accession   NZ_CP046703
Coordinates   158854..159981 (-) Length   375 a.a.
NCBI ID   WP_114080205.1    Uniprot ID   A0A6P1KRE8
Organism   Nostoc sp. ATCC 53789     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 153854..164981
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJB62_RS00550 (GJB62_00545) - 154307..154693 (-) 387 WP_012406942.1 DUF1257 domain-containing protein -
  GJB62_RS00555 (GJB62_00550) - 154733..154942 (-) 210 WP_114080209.1 DUF2997 domain-containing protein -
  GJB62_RS00560 (GJB62_00555) - 155706..156710 (+) 1005 WP_114080208.1 saccharopine dehydrogenase-like oxidoreductase -
  GJB62_RS00565 (GJB62_00560) - 156837..157397 (-) 561 WP_114080207.1 hypothetical protein -
  GJB62_RS00570 (GJB62_00565) - 157526..158749 (-) 1224 WP_114080206.1 type II secretion system F family protein -
  GJB62_RS00575 (GJB62_00570) pilT 158854..159981 (-) 1128 WP_114080205.1 type IV pilus twitching motility protein PilT Machinery gene
  GJB62_RS00580 (GJB62_00575) pilB/pilB1 160123..162129 (-) 2007 WP_114080204.1 GspE/PulE family protein Machinery gene
  GJB62_RS00585 (GJB62_00580) grpE 162588..163337 (+) 750 WP_114080203.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41042.25 Da        Isoelectric Point: 7.4775

>NTDB_id=406665 GJB62_RS00575 WP_114080205.1 158854..159981(-) (pilT) [Nostoc sp. ATCC 53789]
MEMMIEDLMEQLIELGGSDMHLSAGLPPYFRISGKLTPIGEHILTADQCQRLIFSMLNNTQRKTLEQNWELDCSYGVKGL
ARFRVNVYKERGSYAACLRALSSKIPNFEKLGLPDIVREMTDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIVLVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPHERQTQVRVQLSNSLVAVFSQTLVSKKNPKPGEYGRVMAQEILIVTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADFYKSGTISFEAAMSKTSKPDEIQRLIGNSTPQAGAGAKSGAAAKAH

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=406665 GJB62_RS00575 WP_114080205.1 158854..159981(-) (pilT) [Nostoc sp. ATCC 53789]
ATGGAAATGATGATTGAAGATTTGATGGAACAGTTGATTGAACTGGGTGGCTCGGATATGCATTTATCCGCAGGTTTGCC
TCCCTACTTCCGCATCAGTGGCAAACTCACTCCCATCGGTGAGCATATATTGACAGCCGATCAATGTCAAAGGCTGATTT
TTAGTATGCTCAACAACACCCAGCGTAAAACCTTAGAGCAAAACTGGGAACTGGATTGTTCTTATGGGGTTAAAGGTTTG
GCACGTTTCCGGGTCAATGTTTATAAAGAACGTGGTTCTTATGCTGCTTGCTTACGGGCATTAAGTTCTAAGATTCCTAA
CTTTGAAAAATTAGGTCTGCCAGATATCGTAAGGGAAATGACAGATAAGCCCAGAGGACTGATTCTGGTGACAGGGCCTA
CAGGTTCCGGGAAGACAACTACCCTAGCAGCGATGATCGACTTGATTAACCGCACTAAAGCAGAGCATATTTTAACGGTG
GAAGACCCAATTGAATTTGTCTACGAACCAATTAAAAGCTTGGTTCACCAACGACAACTGGGTGAAGATACTAAGAGCTT
TGCTAACGCTTTGAAAGCAGCTTTGCGTGAAGATCCAGATATTGTTCTGGTGGGAGAAATGCGCGATTTAGAAACAATTT
CTTTGGCGATTTCCGCAGCAGAAACAGGACACTTGGTATTTGGTACTCTCCACACCAGTTCTGCCGCCCAGACAGTTGAT
CGGATTATTGACGTTTTCCCCCATGAAAGACAAACCCAGGTACGGGTGCAGTTATCTAACTCTTTAGTGGCAGTATTTAG
CCAAACGTTGGTGTCCAAGAAAAACCCGAAACCTGGTGAATATGGTCGGGTGATGGCTCAAGAAATTCTGATTGTCACTC
CTGCTATTTCCAACTTGATTCGAGAAGGCAAAACATCTCAAATTTACTCAGCTATTCAAACTGGCGGCAAATTGGGGATG
CAAACTCTGGAGAAAGTTTTAGCTGATTTTTACAAATCGGGAACGATTTCCTTTGAAGCCGCGATGTCTAAAACTTCTAA
GCCAGATGAAATCCAACGTCTCATCGGGAATTCTACACCGCAAGCAGGCGCAGGTGCAAAATCCGGTGCGGCTGCCAAAG
CGCATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P1KRE8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.467

92.533

0.504
  pilT Pseudomonas stutzeri DSM 10701

53.868

93.067

0.501
  pilT Legionella pneumophila strain ERS1305867

52.738

92.533

0.488
  pilT Acinetobacter baylyi ADP1

52.738

92.533

0.488
  pilT Legionella pneumophila strain Lp02

52.738

92.533

0.488
  pilT Acinetobacter nosocomialis M2

51.873

92.533

0.48
  pilT Acinetobacter baumannii D1279779

51.873

92.533

0.48
  pilT Acinetobacter baumannii strain A118

51.873

92.533

0.48
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.411

97.867

0.464
  pilT Neisseria gonorrhoeae MS11

50.737

90.4

0.459
  pilT Neisseria meningitidis 8013

50.442

90.4

0.456
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.415

91.2

0.451
  pilT Vibrio cholerae strain A1552

49.415

91.2

0.451

Multiple sequence alignment