Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   GJB62_RS00580 Genome accession   NZ_CP046703
Coordinates   160123..162129 (-) Length   668 a.a.
NCBI ID   WP_114080204.1    Uniprot ID   -
Organism   Nostoc sp. ATCC 53789     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 155123..167129
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJB62_RS00560 (GJB62_00555) - 155706..156710 (+) 1005 WP_114080208.1 saccharopine dehydrogenase-like oxidoreductase -
  GJB62_RS00565 (GJB62_00560) - 156837..157397 (-) 561 WP_114080207.1 hypothetical protein -
  GJB62_RS00570 (GJB62_00565) - 157526..158749 (-) 1224 WP_114080206.1 type II secretion system F family protein -
  GJB62_RS00575 (GJB62_00570) pilT 158854..159981 (-) 1128 WP_114080205.1 type IV pilus twitching motility protein PilT Machinery gene
  GJB62_RS00580 (GJB62_00575) pilB/pilB1 160123..162129 (-) 2007 WP_114080204.1 GspE/PulE family protein Machinery gene
  GJB62_RS00585 (GJB62_00580) grpE 162588..163337 (+) 750 WP_114080203.1 nucleotide exchange factor GrpE -
  GJB62_RS00590 (GJB62_00585) dnaK 163564..165522 (+) 1959 WP_114080202.1 molecular chaperone DnaK -

Sequence

Protein


Download         Length: 668 a.a.        Molecular weight: 74551.42 Da        Isoelectric Point: 5.1746

>NTDB_id=406666 GJB62_RS00580 WP_114080204.1 160123..162129(-) (pilB/pilB1) [Nostoc sp. ATCC 53789]
MTYSSPQRRSTALTTRTEFSPFGNKLVQSGYVNTEQMRQALIESRKSGRPLTEVLESITGQQLSPELLRQYKKQQLFELK
ILYGVEFLDPEVNSIGNTMMGNLIETLIPVDICRRHRLVPLSKHEDQTPPSVLVAMVAPDNLEASDDLNRILRPQGLALQ
RMVITQEDYQQLINQYLDEMAVRQKHLEQEKFTDINQDLENLGNLDLADAPEEMEADLGAAMKGAEDAPVINLVNRILAK
ALHEGVSDIHIEPQEENLRIRFRKDGVLKEAFDPLPKKIIPAVTARFKIIANLDIAERRLPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLDKLITDPETLHIVQDMVSRPFGLILVTGPTGSGKTTSLYSALSEKNDPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCSECRIPYTPTTEELARYGLSASSDVAITFYKANSLTLDAIAEAKGKNQLCSKCNG
VGYKGRCGVYEVMRVTETLQTLINEDAPTERIKEVAIEEGMKTLLAYSLDLVREGSTTLEEVERVTFTDTGLEAELKAKR
KTGLTCRSCDATLKQEWLDCPYCMTSRF

Nucleotide


Download         Length: 2007 bp        

>NTDB_id=406666 GJB62_RS00580 WP_114080204.1 160123..162129(-) (pilB/pilB1) [Nostoc sp. ATCC 53789]
ATGACTTACTCGTCACCACAACGGCGCAGTACCGCTCTAACTACCAGAACAGAGTTTTCGCCCTTTGGCAACAAGCTAGT
GCAATCTGGCTATGTCAATACCGAACAGATGAGGCAGGCATTAATTGAAAGCCGCAAATCTGGCCGACCCTTAACGGAAG
TACTAGAGTCAATTACTGGGCAACAACTATCACCTGAGTTGCTCAGGCAATACAAAAAACAGCAGCTATTTGAACTTAAA
ATACTATACGGTGTTGAATTCCTCGATCCGGAAGTCAACTCCATTGGCAACACGATGATGGGGAACCTGATTGAAACCCT
CATCCCAGTGGATATCTGCCGTCGCCATCGTTTAGTACCACTATCGAAACACGAAGACCAAACCCCGCCCTCAGTTTTAG
TGGCGATGGTCGCTCCAGATAATCTAGAGGCTTCCGATGACCTGAACCGCATCTTGCGTCCCCAAGGGCTGGCATTACAG
CGCATGGTGATTACACAGGAAGACTACCAGCAGCTAATCAACCAATATCTGGATGAAATGGCTGTTCGGCAAAAACACCT
GGAACAAGAAAAGTTTACAGATATTAATCAGGATTTAGAAAACCTCGGAAATCTTGATCTTGCAGATGCTCCTGAAGAAA
TGGAGGCTGATTTAGGAGCAGCGATGAAGGGTGCGGAGGATGCCCCAGTCATCAACCTAGTCAACAGAATCCTTGCTAAA
GCCTTACATGAGGGCGTTTCTGACATTCATATCGAACCGCAAGAAGAAAACTTACGCATCCGCTTTCGGAAGGATGGGGT
ACTGAAGGAAGCTTTCGATCCCCTACCGAAAAAAATCATCCCGGCGGTGACAGCCCGATTTAAAATCATCGCCAATCTAG
ACATTGCTGAACGCCGTCTACCCCAAGATGGACGTATCCGCCGGATGTTTGAGGGACGTAAGGTGGACTTCCGGGTGAAT
ACCTTGCCCAGCCGCTACGGGGAAAAGGTGGTACTGCGGATTTTGGATAACTCCTCCACCCAACTGGGATTAGATAAGTT
AATTACCGATCCAGAGACTTTACATATTGTCCAGGATATGGTTAGCCGTCCCTTTGGGCTAATTTTGGTAACTGGGCCAA
CTGGTTCTGGGAAAACAACCTCGTTGTATTCTGCACTATCAGAAAAGAACGATCCCGGAATTAATATCAGTACTGTGGAA
GACCCAATTGAGTACAGTCTTCCAGGGATTACTCAAGTACAGGTAATTCGGGAAAAGGGGCTAGATTTTGCTACTGCTCT
GCGGGCTTTCTTGCGACAAGATCCAGACGTGCTACTGGTGGGTGAAACGCGGGATAAGGAAACGGCAAAAACAGCGATTG
AAGCTGCCTTGACTGGTCACTTAGTATTAACTACTTTACATACCAATGATGCCCCAGGTGCGATCGCTCGTTTGGGAGAA
ATGGGGATTGAGCCTTTCATGGTTTCTAGTTCGCTAATTGGCGTTCTAGCTCAACGTTTGGTGCGGCGTGTATGTTCTGA
ATGTCGTATTCCCTACACTCCTACAACCGAGGAACTGGCTCGTTATGGTCTATCAGCTTCTTCTGATGTCGCAATTACCT
TCTATAAGGCTAACAGTTTGACATTAGATGCGATCGCCGAAGCCAAAGGCAAAAATCAGCTTTGCTCAAAATGTAATGGT
GTTGGCTACAAAGGTCGTTGTGGTGTTTATGAAGTCATGCGAGTCACCGAAACCCTGCAAACTCTTATCAACGAAGATGC
ACCCACAGAACGCATCAAGGAAGTGGCAATAGAAGAGGGCATGAAAACTTTGCTGGCTTACAGCTTGGACTTAGTGCGCG
AGGGTTCTACCACTTTAGAAGAAGTGGAACGAGTGACGTTTACTGATACTGGTTTGGAAGCCGAGTTAAAAGCCAAACGC
AAGACTGGCCTTACCTGCCGCAGTTGTGATGCCACATTGAAACAAGAATGGCTGGATTGTCCTTACTGTATGACATCTCG
GTTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(64-181)

Type II/IV secretion system protein (T2SSE)

(236-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.667

100

0.671

Multiple sequence alignment