Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   GOM48_RS00925 Genome accession   NZ_CP046523
Coordinates   182227..183168 (+) Length   313 a.a.
NCBI ID   WP_235097792.1    Uniprot ID   -
Organism   Streptococcus oralis strain SOD     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 177227..188168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GOM48_RS00905 (GOM48_00890) tgt 177266..178408 (+) 1143 WP_084946823.1 tRNA guanosine(34) transglycosylase Tgt -
  GOM48_RS00910 (GOM48_00895) - 178542..180359 (+) 1818 WP_084946820.1 acyltransferase family protein -
  GOM48_RS00915 (GOM48_00900) nagA 180500..181651 (+) 1152 WP_084945412.1 N-acetylglucosamine-6-phosphate deacetylase -
  GOM48_RS00920 (GOM48_00905) - 181800..182165 (+) 366 WP_000286399.1 DUF1033 family protein -
  GOM48_RS00925 (GOM48_00910) comGA/cglA/cilD 182227..183168 (+) 942 WP_235097792.1 competence type IV pilus ATPase ComGA Machinery gene
  GOM48_RS00930 (GOM48_00915) comGB/cglB 183116..184132 (+) 1017 WP_235097794.1 competence type IV pilus assembly protein ComGB Machinery gene
  GOM48_RS00935 (GOM48_00920) comGC/cglC 184134..184457 (+) 324 WP_235097796.1 competence type IV pilus major pilin ComGC Machinery gene
  GOM48_RS00940 (GOM48_00925) comGD/cglD 184420..184854 (+) 435 WP_235097798.1 competence type IV pilus minor pilin ComGD Machinery gene
  GOM48_RS00945 (GOM48_00930) comGE/cglE 184817..185119 (+) 303 WP_235097800.1 competence type IV pilus minor pilin ComGE Machinery gene
  GOM48_RS00950 (GOM48_00935) comGF/cglF 185082..185543 (+) 462 WP_002875275.1 competence type IV pilus minor pilin ComGF Machinery gene
  GOM48_RS00955 (GOM48_00940) comGG/cglG 185521..185934 (+) 414 WP_235097802.1 competence type IV pilus minor pilin ComGG Machinery gene
  GOM48_RS00960 (GOM48_00945) - 185967..186557 (+) 591 WP_235097805.1 class I SAM-dependent methyltransferase -
  GOM48_RS00965 (GOM48_00950) comYH 186616..187569 (+) 954 WP_235097806.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35599.55 Da        Isoelectric Point: 5.6227

>NTDB_id=404887 GOM48_RS00925 WP_235097792.1 182227..183168(+) (comGA/cglA/cilD) [Streptococcus oralis strain SOD]
MVQEIAQKIIATAKEKKAQDIYFIPKEKSYELHMRIGDERCLVDSYEFDVLAAVISHFKFVAGMNVGEKRRSQLGSCDYQ
YDEKVSSLRLSTVGDYRGHESLVIRLLHDEERDLHFWFQDIGELGKQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQEDMLQLQLNETIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVTEEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQPTSWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=404887 GOM48_RS00925 WP_235097792.1 182227..183168(+) (comGA/cglA/cilD) [Streptococcus oralis strain SOD]
ATGGTACAAGAAATTGCACAGAAAATTATTGCTACTGCGAAAGAAAAGAAGGCTCAGGATATCTATTTTATCCCCAAGGA
AAAGTCCTACGAGCTTCACATGCGGATTGGAGACGAACGGTGTCTAGTTGACTCCTATGAGTTTGATGTTTTAGCTGCTG
TGATTAGTCATTTCAAGTTTGTGGCTGGGATGAATGTAGGAGAAAAGCGTCGCAGTCAGCTGGGTTCTTGTGATTATCAG
TATGACGAAAAGGTTTCTTCACTGCGTTTGTCCACCGTGGGAGATTATCGGGGACATGAGAGTTTGGTCATTCGTTTGTT
ACACGATGAGGAGCGGGACCTGCATTTCTGGTTTCAGGATATTGGAGAACTGGGCAAGCAATACAGACAAAGGGGTCTCT
ATCTATTTGCAGGTCCAGTCGGAAGTGGCAAGACGACGCTGATGCACGAATTAGCTAAGTCTCTGTTTAAGGGGCAGCAG
GTTATGTCCATCGAAGATCCTGTTGAGATCAAGCAAGAAGACATGCTCCAGTTGCAGTTGAATGAGACGATTGGACTGAC
CTATGAAAATCTGATTAAACTGTCTCTCCGTCATCGTCCTGACCTCTTGATTATCGGTGAAATTCGGGACAGTGAGACGG
CGCGTGCGGTTGTCAGAGCCAGTTTGACAGGTGCGACAGTTTTTTCAACCATTCATGCCAAGAGTATCCGAGGTGTTTAT
GAACGCCTTCTGGAGTTGGGTGTGACGGAGGAGGAACTAGCAGTTGTTCTGCAAGGCGTCTGTTACCAGAGATTAATCGG
GGGAGGAGGAATTGTTGACTTTGCAAACAAAGACTATCAAGAACACCAGCCAACTAGCTGGAATGAGCAGATTGATCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

90.096

100

0.901
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

89.137

100

0.891
  comGA/cglA/cilD Streptococcus pneumoniae D39

89.137

100

0.891
  comGA/cglA/cilD Streptococcus pneumoniae R6

89.137

100

0.891
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

89.137

100

0.891
  comYA Streptococcus gordonii str. Challis substr. CH1

79.355

99.042

0.786
  comYA Streptococcus mutans UA159

67.203

99.361

0.668
  comYA Streptococcus mutans UA140

67.203

99.361

0.668
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.968

99.042

0.604
  comGA Lactococcus lactis subsp. cremoris KW2

54.487

99.681

0.543
  comGA Latilactobacillus sakei subsp. sakei 23K

44.106

84.026

0.371

Multiple sequence alignment