Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   B565_RS17775 Genome accession   NC_015424
Coordinates   3944877..3945911 (-) Length   344 a.a.
NCBI ID   WP_005340354.1    Uniprot ID   -
Organism   Aeromonas veronii B565     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3939877..3950911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B565_RS17745 (B565_3507) glnK 3940461..3940799 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  B565_RS17750 (B565_3508) arfB 3941001..3941417 (+) 417 WP_005348886.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  B565_RS17755 (B565_3509) - 3941448..3941939 (+) 492 WP_005348884.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  B565_RS17760 (B565_3510) - 3942298..3942723 (-) 426 WP_005340360.1 hypothetical protein -
  B565_RS17765 (B565_3511) yaaA 3942918..3943691 (-) 774 WP_005340358.1 peroxide stress protein YaaA -
  B565_RS17770 (B565_3512) pilU 3943739..3944848 (-) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  B565_RS17775 (B565_3513) pilT 3944877..3945911 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  B565_RS17780 (B565_3514) - 3945951..3946652 (+) 702 WP_013724131.1 YggS family pyridoxal phosphate-dependent enzyme -
  B565_RS17785 (B565_3515) proC 3946795..3947619 (+) 825 WP_013724132.1 pyrroline-5-carboxylate reductase -
  B565_RS17790 (B565_3516) - 3947631..3948182 (+) 552 WP_005341416.1 YggT family protein -
  B565_RS17795 (B565_3517) yggU 3948182..3948484 (+) 303 WP_005340341.1 DUF167 family protein YggU -
  B565_RS17800 (B565_3518) - 3948528..3948947 (+) 420 WP_005340339.1 DUF4426 domain-containing protein -
  B565_RS17805 (B565_3519) - 3949049..3950245 (-) 1197 WP_005348874.1 NupC/NupG family nucleoside CNT transporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38273.03 Da        Isoelectric Point: 6.7440

>NTDB_id=40463 B565_RS17775 WP_005340354.1 3944877..3945911(-) (pilT) [Aeromonas veronii B565]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=40463 B565_RS17775 WP_005340354.1 3944877..3945911(-) (pilT) [Aeromonas veronii B565]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGACCATCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTTTCGTTCGAGGTGCCCAATCTGGCCCGT
TTCCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCGGGCGCGGTATTTCGTACCATCCCCAGCACGGTGCTGAGCCTCGA
AGATCTCGATGCGCCGGAGATCTTCCGCAAGATCGCCGAGTTCCCGCGGGGTCTGGTGTTGGTGACCGGCCCGACCGGTT
CCGGTAAGTCGACCACCCTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCATCACCATATCCTCACCATCGAAGAC
CCCATCGAATTCGTCCACGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTGCACCGCGATACCAAGAGCTTCAGCAA
CGCCCTACGCTCGGCACTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGCGACCTTGAGACCATTCGTCTGG
CCATGACCGCCGCCGAAACCGGCCATCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCAGGTGCGGAGAAGGACATGGTGCGCTCCATGCTCTCCGAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGCATTGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATGGGCATTCCGGCTGTGCGTAACCTCATTC
GGGAGGACAAGATTGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGTCGTGGCGTGGTGGCATCCCTCGATGCCAAGGCCAAGGCTGTTGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407

Multiple sequence alignment