Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   B565_RS17770 Genome accession   NC_015424
Coordinates   3943739..3944848 (-) Length   369 a.a.
NCBI ID   WP_005340356.1    Uniprot ID   -
Organism   Aeromonas veronii B565     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3938739..3949848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B565_RS17740 (B565_3506) - 3939195..3940202 (-) 1008 WP_005348888.1 Fe(3+) ABC transporter substrate-binding protein -
  B565_RS17745 (B565_3507) glnK 3940461..3940799 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  B565_RS17750 (B565_3508) arfB 3941001..3941417 (+) 417 WP_005348886.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  B565_RS17755 (B565_3509) - 3941448..3941939 (+) 492 WP_005348884.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  B565_RS17760 (B565_3510) - 3942298..3942723 (-) 426 WP_005340360.1 hypothetical protein -
  B565_RS17765 (B565_3511) yaaA 3942918..3943691 (-) 774 WP_005340358.1 peroxide stress protein YaaA -
  B565_RS17770 (B565_3512) pilU 3943739..3944848 (-) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  B565_RS17775 (B565_3513) pilT 3944877..3945911 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  B565_RS17780 (B565_3514) - 3945951..3946652 (+) 702 WP_013724131.1 YggS family pyridoxal phosphate-dependent enzyme -
  B565_RS17785 (B565_3515) proC 3946795..3947619 (+) 825 WP_013724132.1 pyrroline-5-carboxylate reductase -
  B565_RS17790 (B565_3516) - 3947631..3948182 (+) 552 WP_005341416.1 YggT family protein -
  B565_RS17795 (B565_3517) yggU 3948182..3948484 (+) 303 WP_005340341.1 DUF167 family protein YggU -
  B565_RS17800 (B565_3518) - 3948528..3948947 (+) 420 WP_005340339.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41076.12 Da        Isoelectric Point: 6.5043

>NTDB_id=40462 B565_RS17770 WP_005340356.1 3943739..3944848(-) (pilU) [Aeromonas veronii B565]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=40462 B565_RS17770 WP_005340356.1 3943739..3944848(-) (pilU) [Aeromonas veronii B565]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGGGGGGAGGCGCTCGACAAGAAGGGGGCTCTGACGCTGGTCAGGGATA
CCCTCAGTAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAGGCTCTTGGTCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCTACCTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTCGGCGCCACCGGAG
CGGGCAAATCGACTACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAC
CCGGTGGAGTTCGTCCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTAAAAAGCTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCAGTCAGGAGACCATGGAGTTTG
CCCTGCAATTCGCCGAGACGGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAAGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCCGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACCGACATTA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCGACCGAGCTGGGTATGCAGACCTTCGATCAGGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCACGCTGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGCACCGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36

Multiple sequence alignment