Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   FOB89_RS20930 Genome accession   NZ_CP046329
Coordinates   1330975..1331421 (-) Length   148 a.a.
NCBI ID   WP_011920034.1    Uniprot ID   -
Organism   Shewanella putrefaciens strain FDAARGOS_681     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1325975..1336421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB89_RS06095 (FOB89_06095) pilD 1326340..1327266 (-) 927 WP_011920036.1 prepilin peptidase Machinery gene
  FOB89_RS06100 (FOB89_06100) pilC 1327466..1328731 (-) 1266 WP_011787902.1 type II secretion system F family protein Machinery gene
  FOB89_RS06105 (FOB89_06105) pilB 1329182..1330891 (-) 1710 WP_011920035.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FOB89_RS20930 (FOB89_06110) pilA/pilAI 1330975..1331421 (-) 447 WP_011920034.1 pilin Machinery gene
  FOB89_RS06115 (FOB89_06115) nadC 1332341..1333228 (-) 888 WP_011787905.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FOB89_RS06120 (FOB89_06120) ampD 1333547..1334089 (+) 543 WP_011787906.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FOB89_RS06125 (FOB89_06125) ampE 1334289..1335140 (+) 852 WP_011787907.1 beta-lactamase regulator AmpE -
  FOB89_RS06130 (FOB89_06130) pdhR 1335650..1336402 (+) 753 WP_011787908.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15600.97 Da        Isoelectric Point: 7.6298

>NTDB_id=402749 FOB89_RS20930 WP_011920034.1 1330975..1331421(-) (pilA/pilAI) [Shewanella putrefaciens strain FDAARGOS_681]
MKGLTLKTGLNHKAKGFTLIELMIVVAIIGILAAIALPAYQDYTIKSQINGGLSEVSALKNSFEVAVLDDTDPSLTATAK
GYLGQQAETGTYCNLAVTARTDIECTLKGGNADKVNGKKITLSRTADGVWSCKSDVDEKFLPKGCTTA

Nucleotide


Download         Length: 447 bp        

>NTDB_id=402749 FOB89_RS20930 WP_011920034.1 1330975..1331421(-) (pilA/pilAI) [Shewanella putrefaciens strain FDAARGOS_681]
ATGAAAGGTCTAACGTTAAAAACAGGGTTAAATCATAAGGCTAAAGGTTTTACCCTAATCGAATTAATGATTGTTGTTGC
CATTATTGGTATTTTGGCCGCAATCGCATTACCTGCTTATCAAGATTACACCATTAAATCCCAAATTAACGGAGGATTAT
CAGAAGTATCAGCCTTGAAAAACTCTTTTGAAGTCGCAGTATTGGATGATACGGATCCATCTCTCACCGCTACAGCTAAA
GGTTATTTAGGCCAACAAGCAGAGACTGGTACTTATTGCAATTTAGCAGTGACTGCTCGGACTGACATAGAATGTACTCT
AAAAGGTGGTAATGCAGATAAAGTTAATGGCAAAAAAATTACATTATCACGAACTGCTGACGGTGTATGGAGCTGCAAAA
GTGATGTCGATGAAAAGTTTTTACCTAAAGGTTGTACTACTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(46-146)

Prokaryotic N-terminal methylation motif (N_methyl)

(12-37)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

57.463

90.541

0.52
  pilA Acinetobacter baumannii strain A118

50.36

93.919

0.473
  pilA Pseudomonas aeruginosa PAK

44.371

100

0.453
  pilA/pilAII Pseudomonas stutzeri DSM 10701

50.376

89.865

0.453
  pilA Vibrio cholerae C6706

44.218

99.324

0.439
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.218

99.324

0.439
  pilA Vibrio cholerae strain A1552

44.218

99.324

0.439
  comP Acinetobacter baylyi ADP1

36.424

100

0.372
  pilA2 Legionella pneumophila str. Paris

40

91.216

0.365
  pilA2 Legionella pneumophila strain ERS1305867

40

91.216

0.365

Multiple sequence alignment