Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FOB89_RS06105 Genome accession   NZ_CP046329
Coordinates   1329182..1330891 (-) Length   569 a.a.
NCBI ID   WP_011920035.1    Uniprot ID   -
Organism   Shewanella putrefaciens strain FDAARGOS_681     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1324182..1335891
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB89_RS06080 (FOB89_06080) yacG 1324285..1324494 (-) 210 WP_011920039.1 DNA gyrase inhibitor YacG -
  FOB89_RS06085 (FOB89_06085) zapD 1324652..1325386 (-) 735 WP_011920038.1 cell division protein ZapD -
  FOB89_RS06090 (FOB89_06090) coaE 1325379..1325996 (-) 618 WP_011920037.1 dephospho-CoA kinase -
  FOB89_RS06095 (FOB89_06095) pilD 1326340..1327266 (-) 927 WP_011920036.1 prepilin peptidase Machinery gene
  FOB89_RS06100 (FOB89_06100) pilC 1327466..1328731 (-) 1266 WP_011787902.1 type II secretion system F family protein Machinery gene
  FOB89_RS06105 (FOB89_06105) pilB 1329182..1330891 (-) 1710 WP_011920035.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FOB89_RS20930 (FOB89_06110) pilA/pilAI 1330975..1331421 (-) 447 WP_011920034.1 pilin Machinery gene
  FOB89_RS06115 (FOB89_06115) nadC 1332341..1333228 (-) 888 WP_011787905.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FOB89_RS06120 (FOB89_06120) ampD 1333547..1334089 (+) 543 WP_011787906.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FOB89_RS06125 (FOB89_06125) ampE 1334289..1335140 (+) 852 WP_011787907.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62643.18 Da        Isoelectric Point: 6.4447

>NTDB_id=402734 FOB89_RS06105 WP_011920035.1 1329182..1330891(-) (pilB) [Shewanella putrefaciens strain FDAARGOS_681]
MPTTGLHLGLSTLFIRKGLLNEEQMAGAIAKSRHNKQGLVATLVQGKLVSSRTIAELCYEEYGTPLLDLAEFDIRAIPED
FLNKKLIEKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLAKALEKILEEDITSLDLGGMDEAA
LAGIEITDTDRRNEEPTGDSSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLAGRLSA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQEKLYLEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPECKQAEVIPEHELLRLGF
SPAQITQGFTTFKPVGCEHCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIASQAKAQGMRDLRQSGLLKVLQGVTSI
AEVNRVTSF

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=402734 FOB89_RS06105 WP_011920035.1 1329182..1330891(-) (pilB) [Shewanella putrefaciens strain FDAARGOS_681]
ATGCCAACCACTGGTCTTCATTTAGGTTTATCCACCCTATTTATCCGTAAGGGTCTGCTAAATGAAGAGCAGATGGCAGG
GGCTATTGCAAAATCCCGTCATAATAAACAAGGGTTAGTGGCGACCTTAGTACAAGGCAAGTTAGTCTCATCCCGCACCA
TTGCCGAGCTTTGCTATGAAGAATATGGCACACCCTTACTGGATTTAGCGGAATTTGATATACGAGCCATTCCCGAAGAT
TTTTTAAATAAAAAACTGATTGAAAAACACCGCTGTTTACCGCTATTTAAGCGCGGAAATCGCCTCTATATCGCCACCTC
CGATCCCACCAATATTGCCGCCCTTGAAGATTTTCAATTTAGCGCAGGCCTGCATGCCGAAGCGATTTTGGTTGAAGAAG
ATAAACTCGCTAAAGCCCTTGAAAAAATCCTTGAAGAAGATATTACCAGCCTCGATTTAGGCGGCATGGATGAAGCGGCG
CTGGCGGGGATAGAAATTACCGATACCGATAGACGCAATGAAGAACCAACAGGTGACAGCAGTGACGATGCGCCCATTGT
TATTTATATCAATAAAATTTTAACCGATGCGATTCGTAAGGGTGCCTCCGACTTACACTTTGAACCCTACGAAAAACGCT
ATCGCATCCGTTTTCGGATTGACGGTATTTTGCATGAGGTATCAGAGCCGCCCATTAGCCTTGCGGGTCGTTTATCCGCC
CGTTTAAAGGTGATGTCAAAACTGGATATTGCCGAGCGGCGCGTACCACAGGATGGCCGAATTAAAATGAAGCTCTCCCG
CACTAAGTCTATCGATTTTCGGGTTAGCACCTTGCCTACCCTGTGGGGCGAAAAAATTGTAATGCGGATCTTGGACTCCT
CCTCCGCACAACTAGGTATCGAAAAACTCGGCTACGAGCCCGATCAAGAAAAGTTATACCTTGAAATGCTCGCTAAACCC
CAAGGGATGATTTTAGTCACAGGACCAACTGGCTCGGGTAAAACCGTTTCCCTCTACACGGGGTTAAATATCCTCAATAC
CGCCGAGCGCAATATTTCGACCGCTGAAGATCCGGTAGAAATTAACCTTGAGGGGGTCAACCAAGTTCATATTAACCTAA
AAGCCGGGCTGACATTTGCCTCAGCGCTACGCTCTTTTTTGCGCCAAGACCCCGACGTGGTGATGGTGGGTGAGATACGC
GATCTTGAAACCGCCGAAATTGCCATTAAAGCAGCACAAACGGGACATTTAGTGTTATCGACCCTACACACTAACTCGGC
CGCCGAAACCCTAACCCGTTTAATCAATATGGGTGTACCTGGATACAATATCGCCAGCTCGGTTAACCTTATTATTGCCC
AGCGCCTTGCCCGCAGACTTTGCCCAGAATGCAAACAAGCAGAGGTGATCCCCGAACATGAACTCTTACGTTTAGGTTTT
TCTCCTGCACAAATTACCCAAGGGTTTACTACCTTTAAACCAGTGGGGTGTGAACATTGCTCTGGTGGTTATAAGGGGCG
AGTAGGGATTTATGAAGTGATGAAAATGTCCGATGAAATTGCCCGTACCATTATGGAAGGCGGCAACTCGTTGCAAATTG
CTTCCCAAGCCAAAGCACAAGGTATGCGCGATTTACGTCAATCGGGTTTATTAAAAGTATTACAGGGCGTCACCAGTATT
GCCGAAGTTAATCGGGTGACGAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.893

98.594

0.591
  pilB Acinetobacter baumannii D1279779

59.633

95.782

0.571
  pilB Legionella pneumophila strain ERS1305867

55.83

99.473

0.555
  pilB Vibrio cholerae strain A1552

56.371

91.037

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

51.228

100

0.513
  pilB Vibrio campbellii strain DS40M4

51.06

99.473

0.508
  pilF Neisseria gonorrhoeae MS11

48.41

99.473

0.482
  pilF Thermus thermophilus HB27

39.576

99.473

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.64

98.243

0.38

Multiple sequence alignment