Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GKC70_RS22575 Genome accession   NZ_CP046139
Coordinates   5004591..5005592 (-) Length   333 a.a.
NCBI ID   WP_342649986.1    Uniprot ID   -
Organism   Pseudomonas sp. REB1044     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4999591..5010592
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC70_RS22555 (GKC70_22310) argS 5000837..5002573 (+) 1737 WP_342649984.1 arginine--tRNA ligase -
  GKC70_RS22560 (GKC70_22315) - 5002575..5003273 (+) 699 WP_039582571.1 SPOR domain-containing protein -
  GKC70_RS22565 (GKC70_22320) - 5003325..5003744 (-) 420 WP_039582570.1 TM2 domain-containing protein -
  GKC70_RS22570 (GKC70_22325) - 5003912..5004544 (+) 633 WP_342649985.1 C40 family peptidase -
  GKC70_RS22575 (GKC70_22330) pilT 5004591..5005592 (-) 1002 WP_342649986.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GKC70_RS22580 (GKC70_22335) - 5005658..5006344 (+) 687 WP_342649987.1 YggS family pyridoxal phosphate-dependent enzyme -
  GKC70_RS22585 (GKC70_22340) proC 5006383..5007201 (+) 819 WP_342649988.1 pyrroline-5-carboxylate reductase -
  GKC70_RS22590 (GKC70_22345) - 5007212..5007802 (+) 591 WP_342649989.1 YggT family protein -
  GKC70_RS22595 (GKC70_22350) - 5007891..5009029 (+) 1139 Protein_4428 homoserine O-acetyltransferase -
  GKC70_RS22600 (GKC70_22355) metW 5009037..5009657 (+) 621 WP_029612369.1 methionine biosynthesis protein MetW -
  GKC70_RS22605 (GKC70_22360) - 5009681..5010115 (+) 435 WP_342649990.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 333 a.a.        Molecular weight: 35727.14 Da        Isoelectric Point: 7.8721

>NTDB_id=401588 GKC70_RS22575 WP_342649986.1 5004591..5005592(-) (pilT) [Pseudomonas sp. REB1044]
MDVTDLLARAVKAGASDLHLAAGELPAWRLDGVLQRVALPTLCSDTLEQGLAPWLDADQRQRWRTGDEVDLAVEPPGLGR
VRLSLFRQYHGLAASLRLIGQQAPTVEALELTGVYQALARCRDGLVLVGGATGSGKSSTLAALVAQLGCDRAGHILTLED
PVEFVHGRGRALINQREIGRHSRDFAQGLRSALRQDPDVIMIGELRDLDSIRLALRAAQTGHLVLATVHTRSAASSIDRL
VEVFAAEEKSLIRVMLAESLQLVVAQALVRRVGGGRVAAREVLVATAAVRNLIREGRVAQLPSAMQTGAAQGMVTMEAAL
DRLRARGLIDNLE

Nucleotide


Download         Length: 1002 bp        

>NTDB_id=401588 GKC70_RS22575 WP_342649986.1 5004591..5005592(-) (pilT) [Pseudomonas sp. REB1044]
ATGGACGTGACCGACCTGCTGGCCCGCGCCGTGAAGGCGGGGGCCTCGGACCTGCACTTGGCGGCGGGCGAATTGCCGGC
GTGGCGCCTGGATGGCGTATTGCAGCGCGTGGCGTTGCCGACGTTGTGCAGCGACACGCTGGAACAGGGGCTTGCGCCCT
GGCTCGATGCGGATCAGCGCCAGCGCTGGCGGACGGGCGACGAGGTGGACCTGGCCGTGGAGCCGCCTGGGCTTGGACGC
GTTCGCCTGAGCCTGTTCAGGCAGTATCACGGCCTGGCCGCCAGCCTGCGGCTGATCGGGCAGCAGGCGCCGACGGTCGA
GGCGTTGGAATTGACTGGGGTGTATCAGGCGCTTGCCCGCTGCCGCGATGGCCTGGTGCTGGTGGGTGGGGCGACCGGCA
GTGGCAAGTCCAGCACGCTCGCCGCGCTGGTCGCGCAACTGGGATGCGACCGCGCAGGCCATATCCTGACCCTGGAAGAC
CCGGTTGAGTTCGTACACGGCCGTGGCCGGGCGTTGATCAACCAGCGCGAGATCGGCCGCCACAGCCGAGACTTCGCCCA
AGGCCTGCGCAGCGCACTGCGTCAGGATCCGGATGTGATCATGATCGGCGAACTGCGCGACCTGGACAGCATCCGCCTGG
CCTTGCGCGCAGCGCAGACCGGTCACCTGGTACTGGCCACCGTGCACACGCGCAGCGCTGCCAGCAGCATCGATCGGCTG
GTGGAGGTGTTCGCCGCCGAAGAGAAGTCGCTGATCCGCGTCATGCTCGCCGAATCACTGCAATTGGTGGTGGCGCAGGC
GTTGGTGCGGCGTGTGGGCGGCGGTCGGGTGGCGGCGCGCGAGGTCCTGGTGGCCACCGCGGCGGTCCGCAATTTGATCC
GCGAAGGGCGCGTGGCGCAATTGCCGTCGGCCATGCAGACCGGTGCGGCACAGGGCATGGTGACGATGGAGGCGGCGCTG
GACAGGTTGAGGGCGCGGGGGCTGATCGACAACCTGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

49.848

98.799

0.492
  pilT Acinetobacter baumannii strain A118

49.544

98.799

0.489
  pilT Pseudomonas stutzeri DSM 10701

49.544

98.799

0.489
  pilT Acinetobacter baumannii D1279779

49.544

98.799

0.489
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.091

99.099

0.486
  pilT Vibrio cholerae strain A1552

49.091

99.099

0.486
  pilT Acinetobacter baylyi ADP1

49.091

99.099

0.486
  pilT Acinetobacter nosocomialis M2

49.24

98.799

0.486
  pilT Legionella pneumophila strain Lp02

47.112

98.799

0.465
  pilT Legionella pneumophila strain ERS1305867

47.112

98.799

0.465
  pilT Neisseria meningitidis 8013

46.364

99.099

0.459
  pilT Neisseria gonorrhoeae MS11

46.061

99.099

0.456
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.03

99.099

0.426
  pilU Pseudomonas stutzeri DSM 10701

36.364

99.099

0.36

Multiple sequence alignment