Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GJV85_RS05530 Genome accession   NZ_CP046072
Coordinates   1145322..1146419 (-) Length   365 a.a.
NCBI ID   WP_207562870.1    Uniprot ID   -
Organism   Sulfurimonas aquatica strain H1576     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1140322..1151419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJV85_RS05505 (GJV85_05495) - 1140794..1141408 (+) 615 WP_207562865.1 tetratricopeptide repeat protein -
  GJV85_RS05510 (GJV85_05500) - 1141443..1142624 (+) 1182 WP_207562866.1 aminotransferase class III-fold pyridoxal phosphate-dependent enzyme -
  GJV85_RS05515 (GJV85_05505) - 1142581..1143801 (+) 1221 WP_207562867.1 TolC family protein -
  GJV85_RS05520 (GJV85_05510) - 1143791..1144795 (+) 1005 WP_207562868.1 SAM-dependent methyltransferase -
  GJV85_RS05525 (GJV85_05515) - 1144893..1145291 (+) 399 WP_207562869.1 response regulator -
  GJV85_RS05530 (GJV85_05520) pilT 1145322..1146419 (-) 1098 WP_207562870.1 type IV pilus twitching motility protein PilT Machinery gene
  GJV85_RS05535 (GJV85_05525) - 1146416..1146757 (-) 342 WP_207562871.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  GJV85_RS05540 (GJV85_05530) gatC 1146766..1147056 (-) 291 WP_207562872.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  GJV85_RS05545 (GJV85_05535) - 1147199..1147795 (+) 597 WP_207562873.1 hypothetical protein -
  GJV85_RS05550 (GJV85_05540) - 1147798..1148790 (+) 993 WP_207562874.1 hypothetical protein -
  GJV85_RS05555 (GJV85_05545) - 1148790..1149617 (+) 828 WP_207562875.1 tyrosine-type recombinase/integrase -
  GJV85_RS05560 (GJV85_05550) - 1149614..1150090 (+) 477 WP_207562876.1 HD domain-containing protein -
  GJV85_RS05565 (GJV85_05555) - 1150203..1151018 (+) 816 WP_207562877.1 HDOD domain-containing protein -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 40598.61 Da        Isoelectric Point: 5.6527

>NTDB_id=400910 GJV85_RS05530 WP_207562870.1 1145322..1146419(-) (pilT) [Sulfurimonas aquatica strain H1576]
MSEEIPEAGTKKRLDIQKLLKSVLAFDSSDLHLVVGSEPQIRIDKELRALNLPVMDANDIETMAYSLIEDKQKKIFEEEN
ELDFSFELKDIGRFRANYYRTIHGIACAFRMIPIDIPTLDEYGNPPIFKELIKKEKGLILVTGPTGSGKSTTLASMLHEI
NLTERRHIITVEDPVEFVHKNIKSLFSQRDVGSTTKSFAAALKYSLRQDPDIILIGEMRDAETIGAALTAAETGHLVFGT
LHTNSAPGTINRIIDVFDGDAQAQVRAQLASSLIAVISQTLIPKVGGGKVATQEILITNPAIQNQIREDKVHQLYSQMQL
NQTETHMSTQTQILIDLLQKNIISKENAIKNSNRPEELISMLGAL

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=400910 GJV85_RS05530 WP_207562870.1 1145322..1146419(-) (pilT) [Sulfurimonas aquatica strain H1576]
ATGAGTGAAGAGATACCAGAAGCTGGGACAAAGAAGCGTTTAGACATCCAAAAACTACTAAAGAGTGTTTTAGCGTTTGA
CTCATCTGACCTGCACCTCGTTGTTGGAAGTGAACCCCAAATCAGAATAGATAAAGAGCTTCGTGCCCTTAACCTTCCTG
TAATGGATGCAAATGATATTGAGACTATGGCATACTCTCTTATTGAAGATAAACAGAAGAAGATATTTGAAGAAGAGAAT
GAACTTGACTTCTCATTTGAATTAAAAGACATTGGACGTTTTCGTGCAAACTACTACCGAACTATTCATGGTATAGCTTG
TGCATTTCGTATGATTCCAATAGATATTCCAACACTTGATGAGTATGGAAATCCCCCTATTTTTAAAGAACTTATTAAAA
AAGAAAAAGGTCTCATACTTGTTACAGGACCAACAGGAAGTGGTAAATCAACAACACTTGCATCTATGCTTCACGAGATA
AACTTAACAGAACGCCGTCATATTATCACTGTAGAAGATCCGGTGGAATTTGTTCATAAGAACATAAAATCTCTTTTTTC
ACAACGTGACGTTGGAAGTACCACTAAGTCATTTGCCGCGGCTCTTAAATACTCTTTGAGACAAGATCCTGATATCATAC
TTATTGGAGAAATGAGGGATGCTGAGACTATTGGTGCTGCACTAACCGCTGCAGAGACTGGTCACTTAGTTTTTGGTACG
CTGCATACAAACTCGGCTCCTGGAACCATTAACCGTATTATCGATGTTTTTGATGGAGATGCTCAAGCTCAAGTTAGAGC
ACAACTTGCATCCTCACTGATAGCTGTAATTTCTCAAACACTTATACCAAAAGTTGGTGGGGGAAAGGTAGCAACACAGG
AAATTCTCATCACAAATCCAGCTATTCAAAATCAGATTAGAGAAGATAAAGTTCACCAGCTCTACTCACAAATGCAACTT
AACCAAACAGAAACACATATGTCTACTCAAACACAGATACTTATAGATCTACTTCAGAAAAATATTATTTCTAAAGAGAA
TGCTATTAAAAATTCAAATAGACCAGAAGAACTTATTTCAATGCTAGGTGCCCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(20-283)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

51.884

94.521

0.49
  pilT Pseudomonas aeruginosa PAK

50.725

94.521

0.479
  pilT Legionella pneumophila strain ERS1305867

52.239

91.781

0.479
  pilT Legionella pneumophila strain Lp02

52.239

91.781

0.479
  pilT Pseudomonas stutzeri DSM 10701

50.435

94.521

0.477
  pilT Acinetobacter baumannii D1279779

50

94.795

0.474
  pilT Acinetobacter nosocomialis M2

50

94.795

0.474
  pilT Acinetobacter baumannii strain A118

50

94.795

0.474
  pilT Neisseria gonorrhoeae MS11

49.267

93.425

0.46
  pilT Neisseria meningitidis 8013

48.974

93.425

0.458
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.15

91.233

0.458
  pilT Vibrio cholerae strain A1552

50.15

91.233

0.458
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.954

94.795

0.436
  pilU Acinetobacter baylyi ADP1

38.617

95.068

0.367

Multiple sequence alignment