Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HP434_RS04075 Genome accession   NZ_CP053720
Coordinates   837207..838235 (+) Length   342 a.a.
NCBI ID   WP_172615228.1    Uniprot ID   -
Organism   Escherichia coli strain CP131_Sichuan     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 832207..843235
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HP434_RS04045 (HP434_04045) yggM 832722..833729 (+) 1008 WP_000745217.1 DUF1202 family protein -
  HP434_RS04050 (HP434_04050) hemW 833884..835020 (-) 1137 WP_000239943.1 radical SAM family heme chaperone HemW -
  HP434_RS04055 (HP434_04055) rdgB 835013..835606 (-) 594 WP_001174735.1 XTP/dITP diphosphatase -
  HP434_RS04060 (HP434_04060) yggU 835614..835904 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  HP434_RS04065 (HP434_04065) yggT 835901..836467 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  HP434_RS04070 (HP434_04070) yggS 836485..837189 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  HP434_RS04075 (HP434_04075) pilT 837207..838235 (+) 1029 WP_172615228.1 type IV pilus twitching motility protein PilT Machinery gene
  HP434_RS24190 - 838236..838364 (+) 129 WP_001278270.1 hypothetical protein -
  HP434_RS04080 (HP434_04080) ruvX 838371..838787 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  HP434_RS04085 (HP434_04085) yqgE 838787..839350 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  HP434_RS04090 (HP434_04090) gshB 839459..840409 (-) 951 WP_000593273.1 glutathione synthase -
  HP434_RS04095 (HP434_04095) rsmE 840422..841153 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HP434_RS04100 (HP434_04100) endA 841233..841940 (-) 708 WP_000286500.1 deoxyribonuclease I -
  HP434_RS04105 (HP434_04105) yggI 842035..842532 (-) 498 WP_001300769.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 342 a.a.        Molecular weight: 37697.20 Da        Isoelectric Point: 6.3640

>NTDB_id=393633 HP434_RS04075 WP_172615228.1 837207..838235(+) (pilT) [Escherichia coli strain CP131_Sichuan]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGMILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRLCRCHARCASLSGLQRQ

Nucleotide


Download         Length: 1029 bp        

>NTDB_id=393633 HP434_RS04075 WP_172615228.1 837207..838235(+) (pilT) [Escherichia coli strain CP131_Sichuan]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCATGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGTCGTTGCCATGCCCGATGCGCAAGCTTATCGGGCCTACAAAGGCAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

48.512

98.246

0.477
  pilT Neisseria gonorrhoeae MS11

48.214

98.246

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

95.614

0.468
  pilT Vibrio cholerae strain A1552

48.93

95.614

0.468
  pilT Acinetobacter baumannii D1279779

45.858

98.83

0.453
  pilT Acinetobacter baumannii strain A118

45.858

98.83

0.453
  pilT Acinetobacter nosocomialis M2

45.858

98.83

0.453
  pilT Pseudomonas stutzeri DSM 10701

45.672

97.953

0.447
  pilT Acinetobacter baylyi ADP1

45

99.415

0.447
  pilT Pseudomonas aeruginosa PAK

45.373

97.953

0.444
  pilT Legionella pneumophila strain Lp02

44.118

99.415

0.439
  pilT Legionella pneumophila strain ERS1305867

44.118

99.415

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

92.982

0.389
  pilU Vibrio cholerae strain A1552

40.062

94.152

0.377

Multiple sequence alignment