Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   FIU98_RS04020 Genome accession   NZ_CP045359
Coordinates   877223..878440 (-) Length   405 a.a.
NCBI ID   WP_152487865.1    Uniprot ID   -
Organism   Pseudomonas sp. THAF42     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 872223..883440
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU98_RS03980 (FIU98_03930) - 872825..872998 (-) 174 WP_047592478.1 DUF3094 domain-containing protein -
  FIU98_RS03985 (FIU98_03935) - 873023..873571 (-) 549 WP_037046217.1 MOSC domain-containing protein -
  FIU98_RS03990 (FIU98_03940) - 873568..874197 (-) 630 WP_037046220.1 DUF1780 domain-containing protein -
  FIU98_RS03995 (FIU98_03945) - 874317..875006 (+) 690 WP_037046222.1 energy-coupling factor ABC transporter permease -
  FIU98_RS04000 (FIU98_03950) - 875111..875326 (+) 216 WP_152487862.1 hypothetical protein -
  FIU98_RS04005 (FIU98_03955) yacG 875381..875575 (-) 195 WP_037046227.1 DNA gyrase inhibitor YacG -
  FIU98_RS04010 (FIU98_03960) coaE 875572..876180 (-) 609 WP_152487863.1 dephospho-CoA kinase -
  FIU98_RS04015 (FIU98_03965) pilD 876350..877219 (-) 870 WP_152487864.1 A24 family peptidase Machinery gene
  FIU98_RS04020 (FIU98_03970) pilC 877223..878440 (-) 1218 WP_152487865.1 type II secretion system F family protein Machinery gene
  FIU98_RS04025 (FIU98_03975) pilB 878443..880146 (-) 1704 WP_152487866.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FIU98_RS25085 (FIU98_03980) pilA 880368..880781 (+) 414 WP_152487867.1 pilin Machinery gene
  FIU98_RS04035 (FIU98_03985) pilA 880885..881301 (+) 417 WP_152487868.1 pilin Machinery gene
  FIU98_RS04040 (FIU98_03990) - 881318..883228 (+) 1911 WP_152487869.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44195.91 Da        Isoelectric Point: 9.8560

>NTDB_id=393633 FIU98_RS04020 WP_152487865.1 877223..878440(-) (pilC) [Pseudomonas sp. THAF42]
MAEKALKTSAFKWEGKDRRGAAVKGVMNGQNPALIKAQLRKQGINPTKVRKESSLSFSKGKKVKPMDIALFTRQMATMMK
AGVPLLQSFDIISEGFDNPNMRKLVDEVKQEVAAGNSFAASLRKKPEYFDELYCNLVESGEQAGALENLLDRIATYKEKT
EALKAKIKKAMTYPIAVIVVAVIVSAILLIKVVPQFQEVFAGFGAELPAFTQMVINISEVLQQWWFIFVVAILALSFAFK
EAMKRSQKLRDSVDRSVLKLPIVGDILYKSAVARFARTLSTTFAAGVPLVDALDSVSGATGNVVFKTATNKVKADVSTGM
QLNFSMRTTGTFPSMAVQMTAIGEESGSLDEMLDKVASFYEAEVDNMVDNLTSLMEPLIMSVLGVLVGGLIIAMYLPIFQ
LGAVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=393633 FIU98_RS04020 WP_152487865.1 877223..878440(-) (pilC) [Pseudomonas sp. THAF42]
ATGGCGGAAAAAGCTCTAAAAACCAGCGCGTTCAAGTGGGAAGGCAAAGACCGACGCGGCGCCGCCGTCAAGGGCGTCAT
GAATGGCCAGAATCCTGCGCTGATCAAGGCACAGTTGCGCAAGCAGGGCATCAACCCGACCAAGGTGCGCAAGGAATCCT
CTCTGTCCTTCAGCAAGGGCAAGAAGGTCAAGCCGATGGATATCGCCCTGTTTACCCGACAGATGGCGACCATGATGAAG
GCCGGCGTACCACTGCTGCAGTCTTTCGACATCATCTCCGAAGGATTCGACAACCCCAACATGCGCAAGCTGGTGGATGA
GGTGAAACAGGAGGTGGCCGCAGGTAACAGCTTCGCTGCCTCGCTACGCAAGAAGCCCGAATATTTCGACGAGCTTTACT
GCAACCTGGTGGAGTCCGGTGAACAGGCGGGTGCACTGGAAAACCTGTTGGATCGTATTGCCACCTACAAGGAAAAGACC
GAGGCACTGAAGGCCAAGATCAAGAAAGCGATGACCTATCCTATTGCAGTCATCGTGGTTGCGGTCATCGTATCGGCAAT
TCTCTTGATCAAAGTGGTCCCACAGTTTCAGGAGGTGTTTGCCGGTTTTGGTGCGGAGCTACCAGCTTTCACTCAGATGG
TGATCAATATCTCCGAGGTTCTCCAGCAATGGTGGTTTATCTTCGTGGTCGCCATATTGGCCTTGTCCTTTGCGTTCAAG
GAGGCAATGAAACGCTCCCAGAAGCTACGCGACTCGGTTGACCGCAGCGTACTCAAGCTCCCAATCGTTGGAGACATTCT
CTATAAATCCGCAGTAGCGCGCTTCGCACGAACACTCTCAACCACGTTTGCTGCGGGCGTACCTCTGGTAGACGCCCTTG
ACTCCGTATCCGGTGCCACAGGCAACGTGGTGTTCAAAACCGCAACCAACAAAGTCAAGGCAGATGTCTCAACGGGTATG
CAGCTGAATTTTTCAATGCGGACTACTGGCACCTTCCCGTCAATGGCAGTGCAAATGACGGCCATTGGCGAAGAGTCAGG
CTCGCTGGATGAAATGCTCGATAAAGTCGCCAGCTTTTATGAAGCCGAAGTGGACAACATGGTTGATAACCTCACCAGCC
TGATGGAACCGCTCATCATGTCTGTACTCGGCGTGCTAGTGGGCGGTCTGATTATTGCGATGTATCTACCTATCTTCCAG
CTGGGTGCTGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.012

100

0.79
  pilC Acinetobacter baumannii D1279779

60

100

0.6
  pilC Acinetobacter baylyi ADP1

59.852

100

0.6
  pilC Legionella pneumophila strain ERS1305867

52.273

97.778

0.511
  pilG Neisseria gonorrhoeae MS11

43.704

100

0.437
  pilG Neisseria meningitidis 44/76-A

43.704

100

0.437
  pilC Vibrio cholerae strain A1552

42.469

100

0.425
  pilC Vibrio campbellii strain DS40M4

42.065

98.025

0.412
  pilC Thermus thermophilus HB27

36.75

98.765

0.363

Multiple sequence alignment