Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GXM_RS23070 Genome accession   NZ_CP045226
Coordinates   5112496..5113623 (+) Length   375 a.a.
NCBI ID   WP_152590081.1    Uniprot ID   -
Organism   Nostoc sphaeroides CCNUC1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5107496..5118623
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GXM_RS23060 (GXM_05623) grpE 5109246..5109995 (-) 750 WP_118166983.1 nucleotide exchange factor GrpE -
  GXM_RS23065 (GXM_05624) pilB/pilB1 5110364..5112367 (+) 2004 WP_118166982.1 GspE/PulE family protein Machinery gene
  GXM_RS23070 (GXM_05625) pilT 5112496..5113623 (+) 1128 WP_152590081.1 type IV pilus twitching motility protein PilT Machinery gene
  GXM_RS23075 (GXM_05627) - 5113757..5114980 (+) 1224 WP_152590082.1 type II secretion system F family protein -
  GXM_RS23080 (GXM_05628) - 5115163..5115723 (+) 561 WP_118166979.1 hypothetical protein -
  GXM_RS23085 (GXM_05630) bioU 5115959..5116981 (-) 1023 WP_152590083.1 (S)-8-amino-7-oxononanoate synthase BioU -
  GXM_RS23090 (GXM_05632) - 5117506..5117766 (+) 261 WP_118166977.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  GXM_RS23095 (GXM_05633) - 5117766..5118083 (+) 318 WP_225892250.1 type II toxin-antitoxin system PemK/MazF family toxin -
  GXM_RS23100 (GXM_05634) - 5118314..5118538 (+) 225 WP_118166975.1 DUF433 domain-containing protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41105.44 Da        Isoelectric Point: 7.4775

>NTDB_id=392846 GXM_RS23070 WP_152590081.1 5112496..5113623(+) (pilT) [Nostoc sphaeroides CCNUC1]
MEMMIEDLMEQLIELGGSDMHLSAGLPPYFRISGKLTPIGEHVLTADQCQRLIFSMLNNTQRKTLEQNWELDCSYGVKGL
ARFRVNVYKERGSYAACLRALSSKIPNFEKLGLPDIVREMTDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIVLVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPHERQTQVRVQLSNSLVAVFSQTLVSKKNPKPGEYGRVMAQEILIVTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADFYKAGTISFEAAMSKTSKPDEIQRLIGTSVPPQAVGAKPGATAKAH

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=392846 GXM_RS23070 WP_152590081.1 5112496..5113623(+) (pilT) [Nostoc sphaeroides CCNUC1]
ATGGAAATGATGATTGAAGACCTGATGGAGCAGTTGATTGAATTAGGTGGCTCGGATATGCATTTATCCGCAGGTTTGCC
TCCCTACTTCCGCATCAGTGGCAAACTCACCCCCATCGGTGAGCATGTATTGACAGCCGATCAATGTCAAAGGCTGATTT
TTAGTATGCTCAACAACACCCAGCGTAAAACCTTAGAGCAGAACTGGGAATTGGATTGTTCTTATGGTGTTAAGGGTTTG
GCTCGCTTCCGGGTCAATGTCTATAAAGAACGTGGTTCTTATGCTGCTTGCTTACGGGCATTAAGTTCTAAGATTCCTAA
CTTTGAAAAATTAGGTTTGCCAGATATCGTGCGGGAAATGACAGATAAGCCCAGAGGGCTAATTCTGGTGACAGGCCCTA
CAGGTTCGGGTAAGACAACTACCCTAGCGGCAATGATTGACTTGATTAACCGCACCAAGGCAGAACATATTTTAACAGTG
GAAGATCCAATTGAATTTGTCTACGAACCAATTAAAAGCTTGGTTCACCAACGACAACTGGGTGAAGATACTAAGAGTTT
TGCTAATGCTTTGAAAGCAGCTTTGCGGGAAGATCCAGATATTGTTCTGGTGGGGGAAATGCGCGATTTAGAAACGATTT
CTTTGGCGATTTCGGCAGCAGAAACAGGACACTTGGTATTTGGTACTCTCCACACTAGTTCTGCTGCACAGACAGTTGAC
CGGATTATCGACGTTTTCCCCCATGAAAGACAAACTCAAGTGCGGGTGCAGTTATCTAACTCATTAGTAGCGGTATTTAG
CCAAACTTTGGTATCCAAGAAAAACCCTAAACCCGGTGAATATGGTCGGGTGATGGCTCAAGAAATTCTGATTGTCACTC
CTGCTATTTCCAACTTAATTCGAGAAGGCAAAACATCTCAAATTTACTCGGCTATTCAAACTGGCGGCAAATTGGGAATG
CAAACTCTGGAGAAGGTTTTAGCCGATTTTTACAAAGCCGGAACGATTTCCTTTGAAGCGGCAATGTCTAAGACTTCTAA
GCCAGATGAAATCCAACGTCTCATCGGTACTTCTGTACCACCGCAGGCAGTAGGTGCAAAACCTGGTGCGACGGCTAAAG
CCCATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.467

92.533

0.504
  pilT Pseudomonas stutzeri DSM 10701

53.868

93.067

0.501
  pilT Acinetobacter baylyi ADP1

52.738

92.533

0.488
  pilT Legionella pneumophila strain Lp02

52.738

92.533

0.488
  pilT Legionella pneumophila strain ERS1305867

52.738

92.533

0.488
  pilT Acinetobacter baumannii D1279779

51.873

92.533

0.48
  pilT Acinetobacter nosocomialis M2

51.873

92.533

0.48
  pilT Acinetobacter baumannii strain A118

51.873

92.533

0.48
  pilT Neisseria gonorrhoeae MS11

50.737

90.4

0.459
  pilT Neisseria meningitidis 8013

48.997

93.067

0.456
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.857

93.333

0.456
  pilT Vibrio cholerae strain A1552

49.415

91.2

0.451
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.415

91.2

0.451

Multiple sequence alignment