Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   GXM_RS23065 Genome accession   NZ_CP045226
Coordinates   5110364..5112367 (+) Length   667 a.a.
NCBI ID   WP_118166982.1    Uniprot ID   A0A5P8W6C0
Organism   Nostoc sphaeroides CCNUC1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5105364..5117367
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GXM_RS23050 (GXM_05621) - 5106471..5106737 (+) 267 WP_147337367.1 hypothetical protein -
  GXM_RS23055 (GXM_05622) dnaK 5107060..5109018 (-) 1959 WP_118166984.1 molecular chaperone DnaK -
  GXM_RS23060 (GXM_05623) grpE 5109246..5109995 (-) 750 WP_118166983.1 nucleotide exchange factor GrpE -
  GXM_RS23065 (GXM_05624) pilB/pilB1 5110364..5112367 (+) 2004 WP_118166982.1 GspE/PulE family protein Machinery gene
  GXM_RS23070 (GXM_05625) pilT 5112496..5113623 (+) 1128 WP_152590081.1 type IV pilus twitching motility protein PilT Machinery gene
  GXM_RS23075 (GXM_05627) - 5113757..5114980 (+) 1224 WP_152590082.1 type II secretion system F family protein -
  GXM_RS23080 (GXM_05628) - 5115163..5115723 (+) 561 WP_118166979.1 hypothetical protein -
  GXM_RS23085 (GXM_05630) bioU 5115959..5116981 (-) 1023 WP_152590083.1 (S)-8-amino-7-oxononanoate synthase BioU -

Sequence

Protein


Download         Length: 667 a.a.        Molecular weight: 74395.13 Da        Isoelectric Point: 5.1210

>NTDB_id=392845 GXM_RS23065 WP_118166982.1 5110364..5112367(+) (pilB/pilB1) [Nostoc sphaeroides CCNUC1]
MNSSPQRRSTALTTRTEFSPFGNKLVQSGYVNTEQMRQALIESRKSGRPLTEVLEAITGQQLSPELLRQYKKQQLFELKI
LYGVEFLDPEVNSFGNTEMANLIETLIPVDICRRHRLVPLSKHEDQTPPSVLVAMVAPDNLEASDDLNRILRPQGLALQR
MVITQEDYQQLINQYLDEMAVKQKHLERENSTDISQDLENLGNLDIEDAPEEMEADLGAAMKGAEDAPVINLVNRILAKA
LHEGVSDIHIEPQEENLRIRFRKDGVLREAFPSMPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRLFEGRKVDFRVNT
LPSRYGEKVVLRILDNSSTQLGLDKLITDPETLNIVKDMVSRPFGLILVTGPTGSGKTTSLYSALSEKNDPGINISTVED
PIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGEM
GIEPFMVSSSLIGVLAQRLVRRVCSECRIAYTPTTEELARYGLSASSDVAVSFYKANTLTLDAIAEAKTKNQLCSKCNGV
GYKGRCGVYEVMRVTENLQTLINEDAPTERIKEVAIEEGMKTLLAYSLDLVREGSTTLEEVERVTFTDTGLEAELKAKRK
TGLTCRTCEATLKPEWLDCPYCMTSRF

Nucleotide


Download         Length: 2004 bp        

>NTDB_id=392845 GXM_RS23065 WP_118166982.1 5110364..5112367(+) (pilB/pilB1) [Nostoc sphaeroides CCNUC1]
ATGAACTCGTCACCACAACGGCGCAGTACCGCTCTAACTACCAGAACAGAGTTTTCGCCCTTTGGCAACAAGCTAGTGCA
ATCTGGCTATGTCAATACCGAACAGATGAGGCAGGCACTAATTGAAAGCCGCAAATCTGGCAGACCCTTAACGGAAGTAC
TAGAGGCAATCACTGGGCAACAACTATCACCTGAGTTGCTCAGGCAATACAAAAAACAGCAGCTATTTGAACTAAAAATA
CTATACGGTGTTGAATTCCTTGATCCGGAAGTCAACTCCTTTGGCAACACGGAGATGGCGAACCTGATTGAAACCCTCAT
CCCGGTGGATATTTGCCGTCGCCATCGTTTAGTACCACTATCGAAACACGAAGACCAAACCCCGCCCTCAGTTTTAGTGG
CGATGGTGGCTCCAGATAATCTAGAGGCTTCTGATGACCTAAATCGCATCTTGCGCCCCCAAGGCTTGGCCTTGCAGCGC
ATGGTGATTACCCAGGAAGACTACCAACAGCTAATCAACCAATATTTGGATGAAATGGCTGTTAAGCAAAAGCACCTGGA
ACGAGAAAACTCTACAGATATTAGTCAGGATTTAGAAAATCTCGGAAATCTCGATATTGAGGATGCTCCTGAAGAAATGG
AGGCTGATCTAGGAGCAGCCATGAAGGGTGCAGAGGATGCCCCAGTTATCAACCTAGTCAACAGAATCCTGGCTAAAGCC
TTGCATGAGGGCGTTTCTGACATTCATATCGAACCGCAAGAAGAAAACTTACGCATTCGTTTTCGGAAAGATGGCGTACT
GCGTGAAGCTTTCCCCTCAATGCCGAAAAAAATCATTCCGGCGGTGACAGCTCGATTTAAAATCATCTCCAATCTCGACA
TTGCCGAACGTCGTCTACCACAAGATGGACGCATCCGGCGGCTGTTTGAGGGACGTAAGGTGGACTTCCGTGTGAATACC
TTGCCCAGTCGCTACGGGGAAAAGGTGGTGCTGCGGATTTTGGATAACTCCTCTACCCAACTGGGATTAGATAAGTTAAT
TACCGATCCAGAGACTTTGAATATTGTCAAGGATATGGTCAGCCGTCCCTTTGGTCTAATTTTGGTAACTGGGCCAACTG
GTTCTGGAAAAACAACTTCGCTGTATTCTGCATTATCAGAAAAGAATGATCCCGGAATTAATATTAGTACTGTGGAAGAC
CCCATTGAGTACAGTCTTCCAGGGATTACTCAAGTACAGGTGATTCGGGAAAAAGGGCTGGATTTTGCTACCGCTCTGCG
GGCTTTCTTGCGGCAAGATCCAGATGTGCTGCTGGTGGGTGAAACGCGGGATAAGGAAACGGCAAAAACAGCAATTGAAG
CTGCCTTAACCGGTCACTTGGTATTAACTACCTTACATACCAATGATGCCCCAGGTGCGATCGCTCGTTTGGGAGAAATG
GGGATTGAGCCTTTCATGGTTTCTAGTTCCCTAATTGGCGTTTTAGCTCAACGTTTGGTGCGGCGTGTATGTTCTGAATG
TCGTATTGCCTACACTCCCACAACGGAGGAATTGGCTCGTTATGGTCTATCAGCTTCCTCGGATGTCGCAGTTAGCTTCT
ATAAGGCTAACACCTTGACATTAGATGCGATCGCAGAAGCTAAAACCAAAAATCAGCTTTGCTCAAAATGTAATGGGGTC
GGCTACAAAGGGCGTTGTGGTGTTTATGAAGTCATGCGAGTCACCGAAAACCTGCAAACTCTCATCAACGAAGATGCACC
CACAGAACGCATCAAGGAAGTGGCAATAGAAGAGGGCATGAAAACCTTGCTGGCTTACAGTTTGGACTTAGTGCGCGAAG
GTTCCACCACTTTAGAAGAAGTAGAACGAGTCACGTTTACTGATACCGGTTTGGAAGCCGAGTTAAAAGCCAAACGCAAG
ACTGGTCTTACCTGCCGGACTTGCGAGGCCACATTGAAACCAGAATGGCTCGATTGTCCCTACTGTATGACATCTCGGTT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(235-502)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(63-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P8W6C0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.415

99.1

0.658

Multiple sequence alignment