Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   F9230_RS01355 Genome accession   NZ_CP045099
Coordinates   308012..308431 (+) Length   139 a.a.
NCBI ID   WP_168158658.1    Uniprot ID   A0AAW6JAU9
Organism   Acinetobacter johnsonii strain AJ14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 303012..313431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F9230_RS01335 (F9230_01340) - 304533..305411 (+) 879 WP_234951053.1 metal-dependent hydrolase -
  F9230_RS01340 (F9230_01345) - 305559..306149 (+) 591 WP_058870410.1 LemA family protein -
  F9230_RS01345 (F9230_01350) - 306187..307263 (+) 1077 WP_234951054.1 TPM domain-containing protein -
  F9230_RS01350 (F9230_01355) - 307257..307817 (+) 561 WP_234951055.1 TPM domain-containing protein -
  F9230_RS01355 (F9230_01360) pilA/pilAI 308012..308431 (+) 420 WP_168158658.1 pilin Machinery gene
  F9230_RS01360 (F9230_01365) - 308509..308928 (-) 420 WP_234951056.1 pilin -
  F9230_RS01365 (F9230_01370) - 309087..310466 (-) 1380 WP_234951057.1 O-antigen ligase family protein -
  F9230_RS01370 (F9230_01375) tfpZ 310521..311267 (+) 747 WP_234951058.1 TfpX/TfpZ family type IV pilin accessory protein -
  F9230_RS01375 (F9230_01380) bfr 311327..311791 (-) 465 WP_234951059.1 heteropolymeric bacterioferritin subunit Bfr -
  F9230_RS01380 (F9230_01385) - 312045..312239 (-) 195 WP_010327639.1 bacterioferritin-associated ferredoxin -
  F9230_RS01385 (F9230_01390) - 312394..312777 (-) 384 WP_004691470.1 RidA family protein -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14283.38 Da        Isoelectric Point: 6.2108

>NTDB_id=391852 F9230_RS01355 WP_168158658.1 308012..308431(+) (pilA/pilAI) [Acinetobacter johnsonii strain AJ14]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQDYIAKSQATSGLAEISPAKTQYEVLVNEGKPSSDFTVANVGLKDSDRCT
VAVTAPTSGAATNAITCTLKGSPKVENAVINLGRAADGTWACTLTGTTLDAKYKPGSCS

Nucleotide


Download         Length: 420 bp        

>NTDB_id=391852 F9230_RS01355 WP_168158658.1 308012..308431(+) (pilA/pilAI) [Acinetobacter johnsonii strain AJ14]
ATGAACGCTCAAAAGGGTTTCACTCTCATTGAATTAATGATCGTTGTTGCAATTATCGGTATTCTTGCTGCGATTGCGAT
TCCTGCTTACCAAGATTATATTGCGAAATCACAAGCTACTTCAGGCTTAGCTGAAATTTCTCCAGCTAAGACACAGTACG
AAGTACTTGTAAACGAAGGTAAGCCATCAAGTGACTTTACCGTTGCTAATGTTGGTCTGAAAGATAGTGATCGTTGTACT
GTAGCTGTGACAGCCCCAACTAGTGGCGCTGCAACAAATGCCATCACTTGTACTCTGAAAGGTTCACCAAAAGTAGAAAA
TGCTGTTATTAATCTTGGACGTGCTGCAGATGGTACATGGGCTTGTACTTTAACGGGTACTACTTTAGATGCTAAATATA
AGCCTGGTTCATGTTCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-138)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

51.724

100

0.54
  pilA Acinetobacter baumannii strain A118

50.714

100

0.511
  pilA Pseudomonas aeruginosa PAK

46.053

100

0.504
  pilA/pilAII Pseudomonas stutzeri DSM 10701

48.201

100

0.482
  comP Acinetobacter baylyi ADP1

43.624

100

0.468
  pilA Ralstonia pseudosolanacearum GMI1000

38.608

100

0.439
  pilA2 Legionella pneumophila str. Paris

41.216

100

0.439
  pilA2 Legionella pneumophila strain ERS1305867

40.541

100

0.432
  pilA Vibrio cholerae C6706

39.583

100

0.41
  pilA Vibrio cholerae strain A1552

39.583

100

0.41
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.583

100

0.41
  pilE Neisseria gonorrhoeae MS11

34.416

100

0.381
  pilA Haemophilus influenzae 86-028NP

38.971

97.842

0.381
  pilA Haemophilus influenzae Rd KW20

38.129

100

0.381

Multiple sequence alignment