Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MPQ_RS06725 Genome accession   NC_014733
Coordinates   1407525..1408613 (-) Length   362 a.a.
NCBI ID   WP_013442155.1    Uniprot ID   C6XDB5
Organism   Methylovorus sp. MP688     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1402525..1413613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPQ_RS06700 (MPQ_1366) thiD 1402605..1403441 (+) 837 WP_013442150.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
  MPQ_RS06705 (MPQ_1367) miaA 1403536..1404492 (+) 957 WP_041362138.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  MPQ_RS06710 (MPQ_1368) dapC 1404529..1405710 (-) 1182 WP_013442152.1 succinyldiaminopimelate transaminase -
  MPQ_RS06715 (MPQ_1369) - 1405793..1406671 (+) 879 WP_013442153.1 DMT family transporter -
  MPQ_RS06720 (MPQ_1370) - 1406628..1407425 (-) 798 WP_013442154.1 intradiol ring-cleavage dioxygenase -
  MPQ_RS06725 (MPQ_1371) pilU 1407525..1408613 (-) 1089 WP_013442155.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MPQ_RS06730 (MPQ_1372) - 1408837..1411194 (+) 2358 WP_013442156.1 EAL domain-containing protein -
  MPQ_RS06735 (MPQ_1373) - 1411272..1413365 (-) 2094 WP_013442157.1 glycosyltransferase family 41 protein -

Sequence

Protein


Download         Length: 362 a.a.        Molecular weight: 40226.45 Da        Isoelectric Point: 4.9637

>NTDB_id=38999 MPQ_RS06725 WP_013442155.1 1407525..1408613(-) (pilU) [Methylovorus sp. MP688]
MAAIDITPVLRFMVDKNGSDLFFSTGTPIHIEIEGKTVPVNTQVMAPGMVKEVAYALMSPEQIKEFEATLEMNFAYSVDE
IEDARFRVNIFRQRGDVAIVIRCIKALIPTFEALGLPPILKELIMKPRGLVLVVGSTGSGKSTTLASMIDYRNENTTGHI
LTLEDPIEFIHKHKKSIVDQREIGIDTLSFDNGLKNAMRQAPDVILIGEIRDMETMKHAMAYSETGHLCLATLHANNANQ
AIERIISFFPPEGKAGLLLGLSMNLVAIVSQRLIPGKLTKRAAAIEVLINTPYISELIQKSKLEEIKDVMADNNDIGMCT
FDQSLFRLYSNDRITEENALANADSRNDLSLRIRFADESEES

Nucleotide


Download         Length: 1089 bp        

>NTDB_id=38999 MPQ_RS06725 WP_013442155.1 1407525..1408613(-) (pilU) [Methylovorus sp. MP688]
ATGGCAGCCATTGATATCACGCCGGTTTTGCGTTTCATGGTCGATAAAAACGGATCAGATCTTTTCTTCAGCACAGGTAC
CCCTATCCATATCGAGATTGAGGGCAAAACCGTGCCGGTGAACACGCAAGTGATGGCGCCGGGCATGGTAAAAGAGGTGG
CCTATGCCCTGATGTCCCCCGAGCAGATCAAGGAATTTGAAGCCACGCTGGAAATGAACTTTGCCTATAGCGTGGATGAA
ATCGAAGATGCGCGTTTCCGCGTCAATATCTTTCGCCAGCGTGGCGATGTTGCCATCGTCATTCGCTGTATCAAGGCGCT
GATTCCCACATTTGAAGCGCTGGGGCTGCCACCTATCCTGAAAGAGCTGATCATGAAGCCACGCGGCCTGGTGCTGGTCG
TGGGTTCTACCGGGTCTGGCAAATCCACCACACTCGCATCCATGATCGACTACCGCAATGAAAACACCACCGGCCATATC
CTGACGCTGGAAGACCCGATCGAATTCATTCACAAGCACAAAAAATCCATCGTGGACCAGCGCGAAATCGGCATTGATAC
GCTGTCTTTTGACAATGGCCTGAAAAACGCCATGCGGCAGGCGCCGGATGTGATTTTGATAGGTGAAATTCGCGACATGG
AAACCATGAAGCACGCCATGGCCTACTCCGAAACCGGGCACTTGTGCCTGGCTACCTTGCATGCCAACAACGCCAACCAG
GCCATTGAGCGCATCATTTCCTTTTTCCCGCCCGAGGGCAAAGCCGGTTTGCTGCTGGGGCTTTCCATGAATCTGGTGGC
GATTGTTTCGCAGCGCCTGATCCCGGGCAAGCTGACCAAACGCGCAGCGGCTATCGAGGTGTTGATCAACACGCCTTATA
TCTCCGAGCTGATCCAGAAATCCAAGCTGGAAGAGATCAAGGATGTCATGGCGGACAACAACGATATCGGCATGTGCACT
TTCGACCAGTCGCTGTTCCGCTTGTACAGCAACGACAGGATCACCGAGGAAAATGCGCTGGCAAATGCCGATTCCCGCAA
TGACTTGTCCTTGCGCATCCGGTTTGCGGATGAATCCGAGGAATCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(56-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C6XDB5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.352

98.895

0.528
  pilU Vibrio cholerae strain A1552

48.864

97.238

0.475
  pilU Acinetobacter baylyi ADP1

47.899

98.619

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.149

94.751

0.409
  pilT Legionella pneumophila strain Lp02

43.452

92.818

0.403
  pilT Legionella pneumophila strain ERS1305867

43.452

92.818

0.403
  pilT Acinetobacter baylyi ADP1

42.398

94.475

0.401
  pilT Pseudomonas aeruginosa PAK

42.059

93.923

0.395
  pilT Acinetobacter nosocomialis M2

42.059

93.923

0.395
  pilT Acinetobacter baumannii strain A118

41.765

93.923

0.392
  pilT Acinetobacter baumannii D1279779

41.765

93.923

0.392
  pilT Pseudomonas stutzeri DSM 10701

41.176

93.923

0.387
  pilT Neisseria meningitidis 8013

39.828

96.409

0.384
  pilT Neisseria gonorrhoeae MS11

39.828

96.409

0.384

Multiple sequence alignment