Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   MPQ_RS03060 Genome accession   NC_014733
Coordinates   629145..629585 (-) Length   146 a.a.
NCBI ID   WP_041361986.1    Uniprot ID   -
Organism   Methylovorus sp. MP688     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 624145..634585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPQ_RS03035 (MPQ_0623) - 624773..625660 (-) 888 WP_013441428.1 polyprenyl synthetase family protein -
  MPQ_RS03040 (MPQ_0624) - 625660..625893 (-) 234 WP_013441429.1 exodeoxyribonuclease VII small subunit -
  MPQ_RS03045 (MPQ_0625) - 626060..627463 (+) 1404 WP_013441430.1 hypothetical protein -
  MPQ_RS03050 (MPQ_0626) - 627530..628180 (-) 651 WP_013441431.1 alpha/beta hydrolase -
  MPQ_RS03055 (MPQ_0627) - 628328..629095 (+) 768 WP_013441432.1 3',5'-cyclic-nucleotide phosphodiesterase -
  MPQ_RS03060 pilA2 629145..629585 (-) 441 WP_041361986.1 pilin Machinery gene
  MPQ_RS03065 (MPQ_0628) - 629781..630302 (-) 522 WP_013441433.1 SRPBCC family protein -
  MPQ_RS03070 (MPQ_0629) - 630365..631360 (-) 996 WP_238525337.1 beta-propeller fold lactonase family protein -
  MPQ_RS03075 (MPQ_0630) - 631564..632085 (+) 522 WP_013441435.1 RNA pyrophosphohydrolase -
  MPQ_RS03080 (MPQ_0632) asd 632113..632958 (-) 846 WP_013441436.1 archaetidylserine decarboxylase -
  MPQ_RS03085 (MPQ_0633) ilvC 633112..634128 (-) 1017 WP_013441438.1 ketol-acid reductoisomerase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 14675.74 Da        Isoelectric Point: 4.8777

>NTDB_id=38995 MPQ_RS03060 WP_041361986.1 629145..629585(-) (pilA2) [Methylovorus sp. MP688]
MKNAQQGFTLIELMIVVAIIGILAAVAIPSYQDYTIRSKVTEGLGLADAAKVAVAETNASSGSFPATNAAAGLPESTDIR
GNAVTSVAVGASGVITITYNNTLGGSPTANGMTLSLTPTLNAGSITWACAVSAADLNKYVPANCRV

Nucleotide


Download         Length: 441 bp        

>NTDB_id=38995 MPQ_RS03060 WP_041361986.1 629145..629585(-) (pilA2) [Methylovorus sp. MP688]
ATGAAAAACGCACAGCAAGGTTTTACTCTTATCGAACTGATGATCGTGGTCGCGATTATCGGTATTTTGGCAGCGGTGGC
GATTCCTTCGTACCAAGATTACACGATCCGCTCAAAAGTAACTGAAGGGTTAGGTTTGGCAGATGCTGCAAAAGTTGCTG
TAGCCGAAACTAATGCTTCATCAGGCAGTTTCCCTGCAACAAATGCTGCCGCGGGCCTTCCTGAAAGTACCGACATTAGA
GGTAATGCTGTAACCAGTGTTGCCGTAGGCGCAAGCGGTGTAATTACGATAACTTACAACAACACTCTCGGTGGCTCACC
AACTGCTAACGGTATGACCCTCAGCCTTACGCCAACACTTAATGCGGGGTCAATTACATGGGCATGTGCGGTTAGCGCAG
CCGACCTCAACAAATATGTACCAGCAAATTGTAGAGTTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)

Pilin (bacterial filament) (Pilin)

(37-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

57.639

98.63

0.568
  pilA2 Legionella pneumophila str. Paris

57.639

98.63

0.568
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.234

100

0.479
  pilA Ralstonia pseudosolanacearum GMI1000

42.331

100

0.473
  comP Acinetobacter baylyi ADP1

44.737

100

0.466
  pilE Neisseria gonorrhoeae MS11

42.236

100

0.466
  pilE Neisseria gonorrhoeae strain FA1090

41.25

100

0.452
  pilA/pilA1 Eikenella corrodens VA1

40.625

100

0.445
  pilA Pseudomonas aeruginosa PAK

38.562

100

0.404
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.597

100

0.404
  pilA Vibrio campbellii strain DS40M4

42.857

91.096

0.39
  pilA Haemophilus influenzae 86-028NP

38.889

98.63

0.384
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.716

96.575

0.384
  pilA Haemophilus influenzae Rd KW20

36.242

100

0.37

Multiple sequence alignment