Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FY043_RS25910 Genome accession   NZ_CP043396
Coordinates   5490497..5491495 (-) Length   332 a.a.
NCBI ID   WP_104833796.1    Uniprot ID   -
Organism   Pseudomonas monteilii strain 170620603RE     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5485497..5496495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FY043_RS25890 (FY043_26375) argS 5486701..5488437 (+) 1737 WP_016712503.1 arginine--tRNA ligase -
  FY043_RS25895 (FY043_26380) - 5488439..5489137 (+) 699 WP_164525173.1 SPOR domain-containing protein -
  FY043_RS25900 (FY043_26385) - 5489222..5489638 (-) 417 WP_016712505.1 NINE protein -
  FY043_RS25905 (FY043_26390) - 5489805..5490431 (+) 627 WP_060498059.1 C40 family peptidase -
  FY043_RS25910 (FY043_26395) pilT 5490497..5491495 (-) 999 WP_104833796.1 type IV pilus twitching motility protein PilT Machinery gene
  FY043_RS25915 (FY043_26400) - 5491561..5492247 (+) 687 WP_060498061.1 YggS family pyridoxal phosphate-dependent enzyme -
  FY043_RS25920 (FY043_26405) proC 5492286..5493104 (+) 819 WP_060498062.1 pyrroline-5-carboxylate reductase -
  FY043_RS25925 (FY043_26410) - 5493115..5493705 (+) 591 WP_012274648.1 YggT family protein -
  FY043_RS25930 (FY043_26415) metX 5493794..5494933 (+) 1140 WP_028698440.1 homoserine O-succinyltransferase MetX -
  FY043_RS25935 (FY043_26420) metW 5494941..5495561 (+) 621 WP_003249106.1 methionine biosynthesis protein MetW -
  FY043_RS25940 (FY043_26425) - 5495584..5496018 (+) 435 WP_060498063.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35959.55 Da        Isoelectric Point: 6.6166

>NTDB_id=383318 FY043_RS25910 WP_104833796.1 5490497..5491495(-) (pilT) [Pseudomonas monteilii strain 170620603RE]
MDVTDLLARAVEAGASDLHLAAGQIPMLRLDGELQRMNLPPLVAADLAEGMAPLLAEQQRRQWAEGDELDLALALPALGR
FRLNVFRQLNGPGATFRLIPGRIATLDELGLEDVYQAVAHCRDGLILVGGPTGSGKSSTLATLLDQLNRERALHIITLED
PVEVIHSSQRSLVNQREIGRHSGGFAQGLRSALRQDPDVIMIGELRDLETVRLALRAAETGHLVLATVHTRSAVNSIDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVRRVGGGRVAAREVLVATTAVRNLVREGRTAQLCSVMQAGGAEGMRTMEGAM
RELREKGLFKDL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=383318 FY043_RS25910 WP_104833796.1 5490497..5491495(-) (pilT) [Pseudomonas monteilii strain 170620603RE]
ATGGATGTGACCGACCTGTTGGCCCGTGCCGTGGAGGCGGGGGCTTCGGACCTGCACCTGGCGGCGGGCCAGATACCGAT
GCTGCGCCTGGACGGTGAATTGCAGCGCATGAACTTGCCGCCGCTGGTGGCGGCTGACCTGGCCGAGGGCATGGCGCCCT
TGTTGGCAGAGCAGCAAAGGCGGCAATGGGCCGAGGGTGATGAATTGGACCTGGCGCTGGCGTTGCCCGCGCTCGGCCGC
TTTCGGCTGAACGTGTTTCGCCAGCTGAATGGCCCTGGGGCAACCTTTCGGCTGATCCCCGGGCGGATCGCCACGCTGGA
TGAACTGGGCCTGGAGGATGTGTATCAAGCTGTTGCGCACTGCCGTGATGGCTTGATCCTGGTGGGTGGGCCGACCGGCA
GCGGCAAGTCCAGCACCCTGGCGACGTTGCTCGACCAACTGAACCGCGAGCGGGCGCTACATATCATTACCCTTGAAGAC
CCGGTGGAAGTTATCCACAGCAGCCAGCGCAGCTTGGTCAATCAGCGTGAGATCGGTCGCCACAGCGGCGGCTTCGCCCA
GGGGCTACGCAGTGCCCTGCGTCAGGACCCGGATGTGATCATGATTGGCGAATTGCGCGACCTAGAGACCGTTCGCCTGG
CTTTGCGCGCGGCAGAGACCGGGCATCTGGTGCTGGCGACGGTGCATACGCGTTCGGCCGTAAACAGCATCGATCGGTTG
GTGGAGGTGTTTGCCGCCGAAGAAAAGCCGTTGGTGCGCGCGATGCTGGCCGAGTCGTTGCGCCTGGTGGTGGCGCAGGT
ACTGGTCAGGCGCGTAGGTGGCGGGCGGGTGGCGGCGCGGGAAGTGTTGGTGGCGACGACAGCGGTGCGTAACCTGGTGC
GTGAGGGGCGGACGGCGCAATTGTGTTCGGTGATGCAGGCGGGTGGTGCAGAAGGGATGCGGACGATGGAGGGGGCAATG
CGGGAGCTGAGAGAGAAGGGGCTGTTCAAAGATCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.829

98.795

0.512
  pilT Acinetobacter baylyi ADP1

50.606

99.398

0.503
  pilT Pseudomonas stutzeri DSM 10701

50.61

98.795

0.5
  pilT Acinetobacter nosocomialis M2

50.305

98.795

0.497
  pilT Acinetobacter baumannii D1279779

50.305

98.795

0.497
  pilT Acinetobacter baumannii strain A118

50.305

98.795

0.497
  pilT Legionella pneumophila strain ERS1305867

49.085

98.795

0.485
  pilT Legionella pneumophila strain Lp02

49.085

98.795

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.78

98.795

0.482
  pilT Vibrio cholerae strain A1552

48.78

98.795

0.482
  pilT Neisseria meningitidis 8013

47.273

99.398

0.47
  pilT Neisseria gonorrhoeae MS11

46.97

99.398

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.828

99.699

0.467
  pilU Pseudomonas stutzeri DSM 10701

38.415

98.795

0.38

Multiple sequence alignment