Detailed information NTG0378690

Bioinformatically predicted

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: CS053_RS04365
• Genome accession: NZ_CP042807
• Coordinates: 1050687..1051130 (-)
• Length: 147 a.a.
• NCBI ID: WP_147626508.1
• Uniprot ID: A0A5B9DVN9
• Organism: Rhodanobacter glycinis strain T01E-68
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1040787..1053413	1050687..1051130	within	0

Gene organization within MGE regions

Location: 1040787..1053413

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CS053_RS04330 (CS053_04330)	-	1040787..1041749 (+)	963	WP_147626503.1	rhamnosyltransferase	-
CS053_RS04335 (CS053_04335)	-	1041725..1042774 (-)	1050	WP_147626504.1	glycosyltransferase family 2 protein	-
CS053_RS04340 (CS053_04340)	-	1042744..1043616 (-)	873	WP_253260836.1	NAD-dependent epimerase/dehydratase family protein	-
CS053_RS04345 (CS053_04345)	-	1043662..1044720 (-)	1059	WP_253260837.1	glycosyltransferase family 4 protein	-
CS053_RS04350 (CS053_04350)	-	1044828..1047752 (-)	2925	WP_172624005.1	phosphoethanolamine transferase	-
CS053_RS04355 (CS053_04355)	-	1047749..1048534 (-)	786	WP_172624006.1	phosphoethanolamine transferase domain-containing protein	-
CS053_RS04360 (CS053_04360)	-	1048579..1050753 (-)	2175	WP_147626507.1	tetratricopeptide repeat protein	-
CS053_RS04365 (CS053_04365)	pilE	1050687..1051130 (-)	444	WP_147626508.1	pilin	Machinery gene
CS053_RS04370 (CS053_04370)	-	1051458..1051901 (-)	444	WP_147626509.1	pilin	-
CS053_RS04375 (CS053_04375)	-	1052049..1053413 (-)	1365	WP_147626510.1	glycosyltransferase family 39 protein	-

Sequence

Protein

• Length: 147 a.a.
• Molecular weight: 15295.47 Da
• Isoelectric Point: 7.8131

>NTDB_id=378690 CS053_RS04365 WP_147626508.1 1050687..1051130(-) (pilE) [Rhodanobacter glycinis strain T01E-68]
MKNMQKGFTLIELMIVVAIIAILAAIAIPAYQNYLIRSQVSEGAVLTDGAKTAIAEFYSNTGHFPKNNESAGLPSAVTSI
SGKYVSQVDISGTPGIIEAEFGGQANSAISGKVFALSAMANGGSINWSCTNSATSVDPKYLPSSCRK

Nucleotide

• Length: 444 bp

>NTDB_id=378690 CS053_RS04365 WP_147626508.1 1050687..1051130(-) (pilE) [Rhodanobacter glycinis strain T01E-68]
ATGAAGAACATGCAAAAAGGCTTCACCCTGATCGAACTGATGATCGTCGTGGCGATCATCGCCATTCTTGCCGCCATCGC
TATCCCGGCCTATCAGAACTACCTGATCCGCTCGCAGGTGTCCGAGGGCGCAGTGCTGACCGATGGCGCCAAAACTGCCA
TCGCCGAGTTCTACTCCAACACGGGCCATTTCCCCAAAAACAACGAGTCTGCCGGCCTGCCATCTGCAGTTACGTCGATC
AGCGGCAAGTACGTGAGCCAAGTGGACATCAGCGGCACGCCGGGCATCATTGAAGCCGAGTTCGGCGGCCAGGCCAACTC
CGCGATCAGCGGCAAGGTCTTCGCGCTTTCCGCAATGGCCAATGGCGGCAGCATCAATTGGAGCTGCACCAATTCGGCCA
CCTCGGTGGACCCGAAATATCTGCCCTCGTCCTGCCGCAAGTAA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (37-146)

• Prokaryotic N-terminal methylation motif (N_methyl) (3-27)

3D structure

• AlphaFold DB: A0A5B9DVN9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilE	Neisseria gonorrhoeae strain FA1090	44.72	100	0.49
pilE	Neisseria gonorrhoeae MS11	44.099	100	0.483
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	34.574	100	0.442
pilA	Ralstonia pseudosolanacearum GMI1000	38.095	100	0.435
comP	Acinetobacter baylyi ADP1	40.909	100	0.429
pilA/pilAI	Pseudomonas stutzeri DSM 10701	39.474	100	0.408
pilA/pilA1	Eikenella corrodens VA1	38.312	100	0.401
pilA2	Legionella pneumophila str. Paris	41.549	96.599	0.401
pilA2	Legionella pneumophila strain ERS1305867	40.278	97.959	0.395
pilA	Acinetobacter baumannii strain A118	39.583	97.959	0.388
pilA	Vibrio cholerae C6706	38.889	97.959	0.381
pilA	Vibrio cholerae strain A1552	38.889	97.959	0.381
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	38.889	97.959	0.381
pilA	Pseudomonas aeruginosa PAK	36.184	100	0.374