Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FM071_RS06535 Genome accession   NZ_CP041406
Coordinates   1250199..1251302 (+) Length   367 a.a.
NCBI ID   WP_193109992.1    Uniprot ID   A0A7M1B8E8
Organism   Sulfurimonas paralvinellae strain GO25     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1245199..1256302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FM071_RS06495 (FM071_06440) - 1245300..1246007 (-) 708 WP_193109977.1 sulfite exporter TauE/SafE family protein -
  FM071_RS06500 (FM071_06445) - 1246007..1246510 (-) 504 WP_193109979.1 hypothetical protein -
  FM071_RS06505 (FM071_06450) - 1246546..1247022 (-) 477 WP_193109981.1 HD domain-containing protein -
  FM071_RS06510 (FM071_06455) - 1247019..1247846 (-) 828 WP_193109983.1 tyrosine-type recombinase/integrase -
  FM071_RS06515 (FM071_06460) - 1247846..1248838 (-) 993 WP_193109984.1 hypothetical protein -
  FM071_RS06520 (FM071_06465) - 1248842..1249438 (-) 597 WP_193109986.1 tetratricopeptide repeat protein -
  FM071_RS06525 (FM071_06470) gatC 1249544..1249834 (+) 291 WP_193109988.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  FM071_RS06530 (FM071_06475) - 1249846..1250190 (+) 345 WP_193109990.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  FM071_RS06535 (FM071_06480) pilT 1250199..1251302 (+) 1104 WP_193109992.1 type IV pilus twitching motility protein PilT Machinery gene
  FM071_RS06540 (FM071_06485) - 1251299..1252309 (-) 1011 WP_226960506.1 SAM-dependent methyltransferase -
  FM071_RS06545 (FM071_06490) - 1252318..1253553 (-) 1236 WP_193109994.1 TolC family protein -
  FM071_RS06550 (FM071_06495) - 1253510..1254691 (-) 1182 WP_193109996.1 acetylornithine transaminase -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 41012.95 Da        Isoelectric Point: 5.8706

>NTDB_id=371849 FM071_RS06535 WP_193109992.1 1250199..1251302(+) (pilT) [Sulfurimonas paralvinellae strain GO25]
MSEEVAAPSENAGKKVDIKKLLKSVLAFKSSDLHLVVGSEPQIRIDKELRPLNLPVLSANDVEEMAYSLIEDKQKKEFEE
HNELDFSFELKNIGRFRANYYRTIHGIGCAFRMIPIEIPTLDEYGNPPIFKELVKREKGLILVTGPTGSGKSTTLASMLH
EINMTERRHIITIEDPVEFVHKNNKSLFSQRDVGSNTESFAAALKYSLRQDPDIILIGEMRDAETIGAALTAAETGHLVF
GTLHTNSAPGTINRIIDVFAGEEQAQIRAQLASSLVAVISQTLIPRIGSGKVATQEILITNPAIQNQIREDKVHQIYSQM
QLNQQETFMTTQTQQLIELLQKNVISKENAIKNSNRPEELLKMIEGL

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=371849 FM071_RS06535 WP_193109992.1 1250199..1251302(+) (pilT) [Sulfurimonas paralvinellae strain GO25]
ATGAGTGAAGAAGTCGCAGCACCATCTGAAAATGCAGGTAAGAAAGTAGACATTAAAAAACTACTCAAAAGTGTTTTGGC
ATTCAAATCTTCGGACTTACACCTTGTTGTCGGAAGTGAACCGCAGATCAGAATAGATAAAGAACTACGTCCTCTCAATC
TTCCCGTTTTAAGTGCAAATGATGTCGAAGAGATGGCATACTCTCTCATCGAAGACAAGCAGAAGAAAGAGTTCGAAGAG
CACAATGAACTCGACTTCTCATTCGAGCTGAAAAACATCGGGCGTTTTCGTGCCAACTACTACCGCACCATTCATGGTAT
CGGTTGCGCCTTTCGTATGATTCCTATTGAGATTCCTACGCTTGATGAGTATGGAAATCCTCCGATCTTTAAAGAACTCG
TTAAAAGAGAAAAAGGTCTCATTCTCGTTACCGGTCCTACCGGCTCAGGTAAATCAACAACGCTTGCTTCAATGCTCCAT
GAGATAAACATGACAGAACGCCGTCACATCATCACTATTGAAGATCCGGTCGAGTTCGTTCACAAAAACAACAAATCACT
CTTCTCACAGCGTGATGTCGGAAGTAATACAGAATCTTTTGCTGCCGCACTGAAATACTCACTCCGTCAGGATCCTGACA
TTATCCTCATCGGGGAGATGCGTGATGCCGAAACTATCGGTGCAGCCTTAACAGCCGCAGAAACAGGTCACCTTGTATTT
GGAACACTCCATACAAACTCTGCTCCGGGTACCATTAACCGTATCATCGATGTTTTCGCCGGTGAAGAACAGGCTCAGAT
ACGTGCGCAACTTGCATCTTCTTTGGTTGCTGTTATTTCTCAAACACTTATTCCCCGCATAGGAAGCGGAAAAGTTGCAA
CACAGGAGATTCTCATCACAAATCCTGCGATTCAGAATCAGATTCGTGAAGATAAAGTACACCAGATATACTCACAAATG
CAGCTTAACCAACAAGAGACATTTATGACGACACAAACGCAACAGCTCATAGAACTGCTTCAAAAAAATGTCATCTCCAA
AGAGAATGCCATCAAAAACTCAAACCGTCCGGAAGAGCTGCTTAAAATGATAGAAGGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(24-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7M1B8E8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.187

93.46

0.488
  pilT Acinetobacter nosocomialis M2

51.445

94.278

0.485
  pilT Acinetobacter baumannii D1279779

51.445

94.278

0.485
  pilT Acinetobacter baumannii strain A118

51.445

94.278

0.485
  pilT Legionella pneumophila strain Lp02

53.134

91.281

0.485
  pilT Legionella pneumophila strain ERS1305867

53.134

91.281

0.485
  pilT Acinetobacter baylyi ADP1

51.304

94.005

0.482
  pilT Pseudomonas stutzeri DSM 10701

50.437

93.46

0.471
  pilT Neisseria gonorrhoeae MS11

49.709

93.733

0.466
  pilT Vibrio cholerae strain A1552

51.351

90.736

0.466
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.351

90.736

0.466
  pilT Neisseria meningitidis 8013

49.419

93.733

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.278

95.095

0.45
  pilU Acinetobacter baylyi ADP1

39.481

94.55

0.373

Multiple sequence alignment