Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FKV23_RS13865 Genome accession   NZ_CP041242
Coordinates   3137025..3138137 (+) Length   370 a.a.
NCBI ID   WP_141624383.1    Uniprot ID   A0A514BUH3
Organism   Marilutibacter alkalisoli strain SJ-36     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3132025..3143137
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FKV23_RS13845 (FKV23_13845) - 3133027..3134403 (-) 1377 WP_141624379.1 pyridoxal-phosphate dependent enzyme -
  FKV23_RS13850 (FKV23_13850) - 3135015..3135230 (-) 216 WP_141624380.1 YdcH family protein -
  FKV23_RS13855 (FKV23_13855) - 3135678..3136535 (-) 858 WP_141624381.1 hypothetical protein -
  FKV23_RS13860 (FKV23_13860) - 3136532..3136834 (-) 303 WP_167285261.1 DUF4398 domain-containing protein -
  FKV23_RS13865 (FKV23_13865) pilU 3137025..3138137 (+) 1113 WP_141624383.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FKV23_RS13870 (FKV23_13870) maiA 3138315..3138971 (-) 657 WP_141624384.1 maleylacetoacetate isomerase -
  FKV23_RS13875 (FKV23_13875) - 3139173..3140153 (-) 981 WP_141624385.1 fumarylacetoacetate hydrolase family protein -
  FKV23_RS13880 (FKV23_13880) - 3140343..3141080 (+) 738 WP_141624386.1 LuxR family transcriptional regulator -
  FKV23_RS13885 (FKV23_13885) - 3141196..3141855 (+) 660 WP_141624387.1 acyl-homoserine-lactone synthase -
  FKV23_RS13890 (FKV23_13890) - 3141980..3142795 (-) 816 WP_141624388.1 DUF3050 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41003.24 Da        Isoelectric Point: 6.1917

>NTDB_id=370777 FKV23_RS13865 WP_141624383.1 3137025..3138137(+) (pilU) [Marilutibacter alkalisoli strain SJ-36]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGKLYPLGNTGLPPGMVKKIAYSLMNEGQVPQFERDLELNMALALPDAGR
FRINVFKQRGEVGMVIRAIRSVIPSIEELQLPQVLKDIIMAPRGLVLIVGSTGSGKSTTLASMIDHRNSNTSGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAATMEAAIAFAETGHLCLATLHSNNADQTLERI
LNFFNESAHKNVLMNLALNLKAVVSQRLVVGTDGRRLPAAEVLINTPHIRDLMRRGQVHEIKQAMEETLEEGMESFDQCL
FRLVKEGRVEMEEALKAADSRDGLALKFRLSEGSGAEHDPYADVFQTSEH

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=370777 FKV23_RS13865 WP_141624383.1 3137025..3138137(+) (pilU) [Marilutibacter alkalisoli strain SJ-36]
ATGGACATCGGCTATTTCCTGAAGCTGATGACGGAAAAGAACGCGTCGGACATGTTCCTGACCACCGGCGCGCCGGTCTA
CATCAAGGTCGAGGGCAAACTCTACCCGCTTGGCAACACAGGTCTACCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGAACGAAGGTCAGGTGCCGCAGTTCGAGCGTGATCTTGAGCTGAACATGGCGCTCGCCCTGCCCGACGCCGGCCGC
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGTGCGATCCGCAGCGTGATCCCGTCGATCGA
GGAGCTGCAACTGCCGCAGGTGCTCAAGGACATCATCATGGCCCCGCGCGGGCTGGTGCTGATCGTCGGCTCCACCGGCT
CGGGCAAGTCGACCACGCTGGCGTCGATGATCGACCATCGCAACAGCAACACGTCCGGGCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAGAAGTCGATCGTCAACCAGCGCGAGGTCGGCCTCGACACGCATGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTCGACGCGGCGACCATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCCTGGAGCGCATC
CTCAACTTCTTCAACGAGTCCGCGCACAAGAACGTGCTGATGAACCTTGCCCTGAACCTCAAGGCGGTGGTGTCGCAGCG
GCTGGTGGTCGGTACCGACGGCCGCCGCCTGCCGGCGGCCGAGGTGCTGATCAACACCCCGCACATCCGCGACCTGATGC
GCCGCGGCCAGGTGCACGAGATCAAGCAGGCGATGGAGGAAACGCTGGAGGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGGCTGGTCAAGGAAGGCAGGGTCGAGATGGAAGAAGCGCTCAAGGCCGCCGATTCGCGCGATGGTCTGGCGCTCAA
GTTCCGCCTGTCCGAAGGTTCGGGCGCGGAGCACGATCCGTACGCGGACGTGTTCCAGACCAGCGAACACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A514BUH3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.975

97.027

0.495
  pilU Acinetobacter baylyi ADP1

48.305

95.676

0.462
  pilU Vibrio cholerae strain A1552

47.458

95.676

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.932

98.649

0.443
  pilT Acinetobacter nosocomialis M2

41.42

91.351

0.378
  pilT Pseudomonas aeruginosa PAK

41.42

91.351

0.378
  pilT Acinetobacter baumannii D1279779

41.42

91.351

0.378
  pilT Acinetobacter baumannii strain A118

41.42

91.351

0.378
  pilT Acinetobacter baylyi ADP1

40.588

91.892

0.373
  pilT Pseudomonas stutzeri DSM 10701

40.237

91.351

0.368
  pilT Legionella pneumophila strain Lp02

40.237

91.351

0.368
  pilT Legionella pneumophila strain ERS1305867

40.237

91.351

0.368
  pilT Neisseria gonorrhoeae MS11

38.617

93.784

0.362
  pilT Neisseria meningitidis 8013

38.617

93.784

0.362

Multiple sequence alignment