Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FHQ07_RS06305 Genome accession   NZ_CP040871
Coordinates   1304616..1305731 (+) Length   371 a.a.
NCBI ID   WP_168191483.1    Uniprot ID   -
Organism   Thermomonas aquatica strain SY21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1299616..1310731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FHQ07_RS06285 (FHQ07_06285) - 1299792..1300955 (-) 1164 WP_139716004.1 cystathionine gamma-synthase -
  FHQ07_RS06290 (FHQ07_06290) - 1301118..1302488 (-) 1371 WP_139716005.1 pyridoxal-phosphate dependent enzyme -
  FHQ07_RS06295 (FHQ07_06295) - 1302624..1302839 (-) 216 WP_139716006.1 YdcH family protein -
  FHQ07_RS06300 (FHQ07_06300) - 1303269..1304531 (-) 1263 WP_139716007.1 OmpA family protein -
  FHQ07_RS06305 (FHQ07_06305) pilU 1304616..1305731 (+) 1116 WP_168191483.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FHQ07_RS06310 (FHQ07_06310) maiA 1305739..1306407 (-) 669 WP_139716008.1 maleylacetoacetate isomerase -
  FHQ07_RS06315 (FHQ07_06315) - 1306512..1307501 (-) 990 WP_139716009.1 fumarylacetoacetate hydrolase family protein -
  FHQ07_RS06320 (FHQ07_06320) - 1307568..1308305 (-) 738 WP_168191484.1 2OG-Fe dioxygenase family protein -
  FHQ07_RS06330 (FHQ07_06330) rpsI 1308538..1308930 (-) 393 WP_139716011.1 30S ribosomal protein S9 -
  FHQ07_RS06335 (FHQ07_06335) rplM 1308933..1309361 (-) 429 WP_139716012.1 50S ribosomal protein L13 -
  FHQ07_RS06340 (FHQ07_06340) coq7 1309556..1310200 (+) 645 WP_139716013.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40809.95 Da        Isoelectric Point: 5.7726

>NTDB_id=367751 FHQ07_RS06305 WP_168191483.1 1304616..1305731(+) (pilU) [Thermomonas aquatica strain SY21]
MDIDYFLKLMSEKNASDMFLSSGAPVNIKVEGELIALGSAPLPAGMSKKVAYSLMDERQIAEFERELELNMAIAVAGSGR
FRVNVFQQRGEVGMVIRAIRSDIPSIEELQLPQVLKDIIMAPRGLVLIVGSTGAGKSTTLASMIDHRNTTTSGHILTIED
PIEYLHRHKKSIVNQREVGLDTHSFHAALKNAMREAPDVILIGEIRDATTMEAAIAFAETGHICLATLHSNNADQTIERI
LNFFPEGAHKNVLMNLALNLKAVVSQRLVVGLDGRRLPAAEVLINTPMIRDLLRRGQVHEIKIAMEESLEDGMQSFDQCL
FRLYKEGKIEMEEALKAADSRDGLALKFRLSEGSSGEHDPYADVFNAGAAH

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=367751 FHQ07_RS06305 WP_168191483.1 1304616..1305731(+) (pilU) [Thermomonas aquatica strain SY21]
ATGGATATCGACTACTTCCTCAAGCTGATGTCGGAAAAGAACGCCTCGGACATGTTCCTGAGTTCCGGCGCGCCGGTGAA
CATCAAGGTCGAGGGCGAGCTGATCGCGCTGGGCAGCGCGCCGCTGCCGGCGGGGATGTCGAAGAAGGTGGCCTATTCGC
TGATGGACGAACGCCAGATCGCCGAATTCGAGCGCGAGCTGGAGCTGAACATGGCGATCGCGGTGGCCGGGTCCGGCCGT
TTCCGCGTCAACGTGTTCCAGCAGCGCGGCGAAGTCGGCATGGTGATCCGCGCGATCCGCAGCGACATCCCCAGCATCGA
GGAGCTGCAGCTGCCGCAGGTGCTGAAGGACATCATCATGGCGCCGCGCGGGCTGGTGCTGATCGTCGGCTCCACCGGCG
CCGGCAAGTCGACCACGCTGGCCTCGATGATCGACCACCGCAACACCACCACCTCCGGCCACATCCTCACCATCGAGGAT
CCGATCGAATACCTGCACCGGCACAAGAAGTCGATCGTCAACCAGCGCGAGGTCGGGCTCGACACCCACAGCTTCCACGC
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCGACGCCACCACCATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGCCACATCTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCCGAGGGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGAAGGCGGTGGTCAGCCAGCG
CCTGGTGGTCGGCCTCGACGGCCGCCGCCTGCCGGCCGCGGAGGTCCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTCCACGAAATCAAGATCGCGATGGAAGAGTCGCTGGAAGACGGCATGCAGAGCTTCGACCAGTGCCTG
TTCCGCCTGTACAAGGAAGGCAAGATCGAGATGGAGGAGGCGCTGAAGGCCGCGGATTCGCGCGATGGCCTCGCCCTCAA
GTTCCGCCTGTCCGAAGGCTCCAGCGGCGAGCACGACCCCTACGCCGACGTGTTCAACGCCGGCGCCGCGCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

96.765

0.504
  pilU Vibrio cholerae strain A1552

50

95.418

0.477
  pilU Acinetobacter baylyi ADP1

50

95.418

0.477
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.788

91.375

0.437
  pilT Pseudomonas stutzeri DSM 10701

43.109

91.914

0.396
  pilT Pseudomonas aeruginosa PAK

42.899

91.105

0.391
  pilT Legionella pneumophila strain ERS1305867

42.012

91.105

0.383
  pilT Legionella pneumophila strain Lp02

42.012

91.105

0.383
  pilT Acinetobacter baylyi ADP1

41.471

91.644

0.38
  pilT Acinetobacter baumannii D1279779

41.716

91.105

0.38
  pilT Acinetobacter nosocomialis M2

41.716

91.105

0.38
  pilT Acinetobacter baumannii strain A118

41.716

91.105

0.38
  pilT Neisseria gonorrhoeae MS11

39.481

93.531

0.369
  pilT Neisseria meningitidis 8013

39.481

93.531

0.369

Multiple sequence alignment