Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   FE254_RS04020 Genome accession   NZ_CP040349
Coordinates   918811..919248 (+) Length   145 a.a.
NCBI ID   WP_273257453.1    Uniprot ID   -
Organism   Pseudomonas guguanensis strain HMFL31     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 913811..924248
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FE254_RS04000 (FE254_04035) coaE 913964..914572 (-) 609 WP_059390436.1 dephospho-CoA kinase -
  FE254_RS04005 (FE254_04040) pilD 914792..915661 (-) 870 WP_273257458.1 A24 family peptidase Machinery gene
  FE254_RS04010 (FE254_04045) pilC 915665..916882 (-) 1218 WP_186348772.1 type II secretion system F family protein Machinery gene
  FE254_RS04015 (FE254_04050) pilB 916885..918588 (-) 1704 WP_273257455.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FE254_RS04020 (FE254_04055) pilE 918811..919248 (+) 438 WP_273257453.1 pilin Machinery gene
  FE254_RS04025 (FE254_04060) - 919314..921251 (+) 1938 WP_273257451.1 tetratricopeptide repeat protein -
  FE254_RS04030 (FE254_04065) - 921248..922096 (+) 849 WP_273257449.1 glycosyltransferase family 2 protein -
  FE254_RS04035 (FE254_04070) - 922097..923263 (+) 1167 WP_273257447.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  FE254_RS04040 (FE254_04075) - 923260..923916 (+) 657 WP_273257445.1 acetyltransferase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14758.89 Da        Isoelectric Point: 7.7852

>NTDB_id=363547 FE254_RS04020 WP_273257453.1 918811..919248(+) (pilE) [Pseudomonas guguanensis strain HMFL31]
MKAQIQKGFTLIELMIVVAIIGILAAIALPAYQDYTARSQMSEALSLAGGARTAVTEFWTTEGKFPSTNASAGLAGATSI
TGNYVNNVNVGTTPGTIIATMNTTGVASGIGGQTLTLSAVTTAGSIEWVCKSSAADKLVPANCRN

Nucleotide


Download         Length: 438 bp        

>NTDB_id=363547 FE254_RS04020 WP_273257453.1 918811..919248(+) (pilE) [Pseudomonas guguanensis strain HMFL31]
ATGAAAGCTCAAATTCAAAAAGGTTTTACCCTGATCGAATTGATGATCGTGGTTGCGATCATCGGTATTCTGGCTGCCAT
CGCTCTGCCGGCTTATCAGGATTACACTGCTCGTTCGCAGATGTCTGAAGCTCTGAGTCTGGCTGGCGGTGCGCGTACTG
CGGTGACCGAGTTCTGGACTACTGAGGGTAAGTTCCCTAGCACCAATGCCTCGGCTGGTCTTGCAGGCGCAACCAGTATC
ACTGGTAACTACGTTAACAACGTCAACGTGGGTACAACCCCTGGCACAATCATCGCCACGATGAATACAACTGGTGTTGC
CTCTGGCATCGGTGGCCAGACTCTGACTCTGAGCGCGGTAACAACTGCTGGCTCTATCGAGTGGGTTTGCAAAAGCAGTG
CTGCTGATAAGCTCGTTCCGGCTAACTGCCGTAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(6-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

47.799

100

0.524
  pilE Neisseria gonorrhoeae strain FA1090

47.468

100

0.517
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

42.771

100

0.49
  pilA Ralstonia pseudosolanacearum GMI1000

39.884

100

0.476
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46.528

99.31

0.462
  comP Acinetobacter baylyi ADP1

43.919

100

0.448
  pilA/pilA1 Eikenella corrodens VA1

41.401

100

0.448
  pilA2 Legionella pneumophila str. Paris

44.681

97.241

0.434
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.203

95.172

0.421
  pilA2 Legionella pneumophila strain ERS1305867

42.857

96.552

0.414
  pilA Pseudomonas aeruginosa PAK

38.312

100

0.407

Multiple sequence alignment