Detailed information NTG0363544

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FE254_RS04005
• Genome accession: NZ_CP040349
• Coordinates: 914792..915661 (-)
• Length: 289 a.a.
• NCBI ID: WP_273257458.1
• Uniprot ID: -
• Organism: Pseudomonas guguanensis strain HMFL31
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 909792..920661

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FE254_RS03970 (FE254_04005)	-	911077..911250 (-)	174	WP_273257466.1	DUF3094 family protein	-
FE254_RS03975 (FE254_04010)	-	911275..911823 (-)	549	WP_273257465.1	MOSC domain-containing protein	-
FE254_RS03980 (FE254_04015)	-	911820..912449 (-)	630	WP_004425012.1	DUF1780 domain-containing protein	-
FE254_RS03985 (FE254_04020)	-	912648..913337 (+)	690	WP_273257463.1	energy-coupling factor ABC transporter permease	-
FE254_RS03990 (FE254_04025)	-	913444..913659 (+)	216	WP_112211225.1	hypothetical protein	-
FE254_RS03995 (FE254_04030)	yacG	913773..913967 (-)	195	WP_004425006.1	DNA gyrase inhibitor YacG	-
FE254_RS04000 (FE254_04035)	coaE	913964..914572 (-)	609	WP_059390436.1	dephospho-CoA kinase	-
FE254_RS04005 (FE254_04040)	pilD	914792..915661 (-)	870	WP_273257458.1	A24 family peptidase	Machinery gene
FE254_RS04010 (FE254_04045)	pilC	915665..916882 (-)	1218	WP_186348772.1	type II secretion system F family protein	Machinery gene
FE254_RS04015 (FE254_04050)	pilB	916885..918588 (-)	1704	WP_273257455.1	type IV-A pilus assembly ATPase PilB	Machinery gene
FE254_RS04020 (FE254_04055)	pilE	918811..919248 (+)	438	WP_273257453.1	pilin	Machinery gene

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 31880.08 Da
• Isoelectric Point: 7.1973

>NTDB_id=363544 FE254_RS04005 WP_273257458.1 914792..915661(-) (pilD) [Pseudomonas guguanensis strain HMFL31]
MPVLDFLASHVLAFVLCALLLGLLIGSFLNVVIYRLPVMLQRDWQGQAREVLELPPAPTAQTFNLILPNSCCPNCGHEIK
PWENIPVISYLFLRGKCSNCKTPISKRYPIVELACGLLSGYVAWHFGFTWQAGAMLVLAWGLLAMSLIDADHQILPDVLV
LPLLWLGLIVNYFGLFTSLEDALWGAIAGYLSLWSVYWLFKLITGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMLRMRGRDSSTPMPFGPYLAIAGWIALLWGDVITGSYLKFAGF

Nucleotide

• Length: 870 bp

>NTDB_id=363544 FE254_RS04005 WP_273257458.1 914792..915661(-) (pilD) [Pseudomonas guguanensis strain HMFL31]
ATGCCTGTACTCGACTTCCTGGCCAGCCATGTGCTGGCCTTTGTTTTGTGTGCTTTGTTGCTGGGCCTGCTGATCGGCAG
CTTCCTCAATGTAGTGATCTATCGCCTACCCGTGATGCTGCAACGCGACTGGCAAGGCCAGGCGCGCGAGGTGCTGGAGT
TGCCGCCGGCACCAACCGCTCAGACCTTTAACCTGATCCTGCCCAATTCCTGCTGCCCCAACTGCGGTCATGAGATAAAG
CCCTGGGAGAACATCCCGGTCATCAGCTATTTGTTCCTGCGCGGCAAGTGTTCGAACTGCAAAACCCCGATCAGCAAACG
TTACCCCATCGTCGAACTGGCCTGCGGCCTGCTTTCAGGCTACGTCGCCTGGCACTTCGGCTTTACCTGGCAGGCCGGGG
CGATGCTGGTGCTGGCCTGGGGGCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAGATTCTGCCGGATGTGCTGGTG
CTGCCATTACTGTGGCTGGGCTTGATCGTCAATTACTTCGGGCTTTTCACCAGCCTGGAAGATGCCCTGTGGGGGGCGAT
TGCCGGTTACCTGAGCCTGTGGTCGGTGTATTGGCTGTTCAAGCTGATCACCGGCAAGGAAGGCATGGGTTACGGTGACT
TCAAGCTACTGGCGATGCTCGGCGCCTGGGGTGGCTGGCAGATTCTGCCGTTGACCATTCTGCTGTCGTCACTGGTCGGG
GCGGTGCTTGGGGTGATTATGCTGCGGATGCGCGGTCGCGACTCCAGTACACCGATGCCCTTCGGCCCGTACCTGGCCAT
TGCGGGATGGATCGCCCTGCTTTGGGGTGATGTGATTACCGGCAGTTATCTGAAGTTCGCTGGGTTTTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.65	94.81	0.509
pilD	Acinetobacter baumannii D1279779	51.408	98.27	0.505
pilD	Acinetobacter nosocomialis M2	51.056	98.27	0.502
pilD	Vibrio campbellii strain DS40M4	52.19	94.81	0.495
pilD	Neisseria gonorrhoeae MS11	51.331	91.003	0.467