Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FE254_RS04015 Genome accession   NZ_CP040349
Coordinates   916885..918588 (-) Length   567 a.a.
NCBI ID   WP_273257455.1    Uniprot ID   -
Organism   Pseudomonas guguanensis strain HMFL31     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 911885..923588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FE254_RS03985 (FE254_04020) - 912648..913337 (+) 690 WP_273257463.1 energy-coupling factor ABC transporter permease -
  FE254_RS03990 (FE254_04025) - 913444..913659 (+) 216 WP_112211225.1 hypothetical protein -
  FE254_RS03995 (FE254_04030) yacG 913773..913967 (-) 195 WP_004425006.1 DNA gyrase inhibitor YacG -
  FE254_RS04000 (FE254_04035) coaE 913964..914572 (-) 609 WP_059390436.1 dephospho-CoA kinase -
  FE254_RS04005 (FE254_04040) pilD 914792..915661 (-) 870 WP_273257458.1 A24 family peptidase Machinery gene
  FE254_RS04010 (FE254_04045) pilC 915665..916882 (-) 1218 WP_186348772.1 type II secretion system F family protein Machinery gene
  FE254_RS04015 (FE254_04050) pilB 916885..918588 (-) 1704 WP_273257455.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FE254_RS04020 (FE254_04055) pilE 918811..919248 (+) 438 WP_273257453.1 pilin Machinery gene
  FE254_RS04025 (FE254_04060) - 919314..921251 (+) 1938 WP_273257451.1 tetratricopeptide repeat protein -
  FE254_RS04030 (FE254_04065) - 921248..922096 (+) 849 WP_273257449.1 glycosyltransferase family 2 protein -
  FE254_RS04035 (FE254_04070) - 922097..923263 (+) 1167 WP_273257447.1 DegT/DnrJ/EryC1/StrS family aminotransferase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62513.53 Da        Isoelectric Point: 5.3504

>NTDB_id=363546 FE254_RS04015 WP_273257455.1 916885..918588(-) (pilB) [Pseudomonas guguanensis strain HMFL31]
MNESVSLSGLAKQMVLAELLDEKAAQQAQQQAQRNKLSLVTYLVQNKLVKSRALAELASEQFGVSFLDLNTLDKESQPRD
LISEKLVRQHRVLPLWRRGNKLFVALSDPTNHQAVTDVQFSTGLTTEAILVEDDKLGDAIEKFFDSANSSMDELADVDLD
GLDVEAVDDEKSVGEGGADADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKAYRVRFRTDGILHEVARPPIQLAPRISAR
LKVMAALDISERRKPQDGRIKMKISKTKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEEQKELYMNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDVNISTAEDPVEINLEGINQVNVNPKQGMDFTQALRAFLRQDPDIIMVGEIRD
LDTASIAVKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCSSCKKEVNVPRETLLAEGFP
EDKIGTFKIYGPVGCENCKGGYKGRVGIYEVVKNTPNLQRIIMEEGNSIDIAAQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=363546 FE254_RS04015 WP_273257455.1 916885..918588(-) (pilB) [Pseudomonas guguanensis strain HMFL31]
ATGAACGAAAGCGTATCCCTGTCCGGCTTGGCCAAGCAGATGGTGCTGGCCGAGTTGCTGGACGAAAAAGCCGCCCAGCA
GGCCCAGCAGCAGGCCCAGCGCAACAAGCTGTCGCTGGTCACCTATCTAGTGCAGAACAAGCTGGTAAAAAGTCGTGCAT
TGGCAGAACTAGCCTCGGAGCAGTTCGGCGTTTCCTTTCTCGACCTCAACACCCTCGACAAGGAAAGCCAGCCACGTGAC
CTGATCAGCGAGAAACTGGTACGCCAACACCGCGTGCTGCCGCTATGGCGTCGAGGTAACAAGCTGTTCGTTGCGCTTTC
TGACCCGACCAATCACCAGGCCGTCACCGATGTGCAGTTCAGCACCGGCCTGACCACAGAAGCAATTTTGGTCGAGGACG
ACAAACTCGGCGATGCCATCGAAAAGTTCTTCGACTCTGCCAACAGCAGCATGGACGAGCTGGCAGACGTTGATCTGGAT
GGACTGGATGTCGAGGCGGTTGATGATGAGAAGTCGGTCGGCGAAGGTGGCGCCGATGCTGACGATGCGCCGGTAGTACG
TTTCGTCAACAAGATGCTGCTTGATGCAATAAAAGGTGGTTCTTCCGACCTGCACTTCGAGCCCTACGAAAAAGCCTATC
GGGTACGCTTTCGTACCGACGGCATCCTGCATGAAGTCGCGCGCCCACCCATTCAGTTAGCTCCGCGAATTTCCGCCCGC
CTGAAGGTGATGGCCGCTCTGGATATCTCCGAGCGGCGCAAGCCGCAGGACGGCCGGATCAAGATGAAAATCTCCAAGAC
CAAGGCCATCGATTTCCGCGTCAACACGCTCCCAACGCTCTGGGGCGAGAAGATCGTGATGCGAATTCTCGACCCCACCA
GTGCGCAGATGGGGATCGATGCACTGGGTTACGAGGAAGAACAGAAAGAGCTGTATATGAACGCCCTGAAGCAGCCCCAG
GGCATGATTCTGGTCACCGGCCCCACTGGCTCGGGAAAGACGGTATCGCTCTATACCGGCCTGAACATCCTCAACACACC
AGATGTGAATATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAGGGCATCAACCAGGTCAACGTCAACCCCAAGC
AGGGCATGGACTTTACCCAGGCGCTGCGCGCCTTTCTGCGCCAAGACCCGGACATCATCATGGTCGGGGAAATCCGCGAT
CTGGACACCGCCTCCATTGCCGTCAAGGCCGCACAGACTGGCCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
GGAAACCCTGACCCGCTTGCGCAATATGGGCGTGCCCTCCTTCAACATCGCTACCTCGGTGAACCTGATCATCGCCCAGC
GCCTGGCGCGCAAGCTCTGCAGCAGTTGCAAGAAGGAAGTCAACGTACCGCGTGAAACCCTTCTGGCCGAAGGCTTCCCC
GAAGACAAGATCGGCACCTTCAAGATTTATGGCCCGGTGGGCTGTGAAAACTGCAAGGGCGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCCAACCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGATATTGCCGCGC
AGATGCGTAAAGACGGCTTTAATGACCTGCGCACCTCCGCTTTGCTCAAGGCCATGCAAGGGGTCACCAGCCTTGAGGAA
GTCAACCGCGTAACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.364

99.824

0.593
  pilB Acinetobacter baumannii D1279779

58.319

100

0.587
  pilB Legionella pneumophila strain ERS1305867

54.306

100

0.545
  pilB Vibrio cholerae strain A1552

49.559

100

0.496
  pilF Neisseria gonorrhoeae MS11

48.673

99.647

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

49.817

96.296

0.48
  pilB Vibrio campbellii strain DS40M4

49.177

96.473

0.474
  pilF Thermus thermophilus HB27

37.719

100

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.265

93.298

0.376

Multiple sequence alignment