Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   FE254_RS04010 Genome accession   NZ_CP040349
Coordinates   915665..916882 (-) Length   405 a.a.
NCBI ID   WP_186348772.1    Uniprot ID   -
Organism   Pseudomonas guguanensis strain HMFL31     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 910665..921882
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FE254_RS03970 (FE254_04005) - 911077..911250 (-) 174 WP_273257466.1 DUF3094 family protein -
  FE254_RS03975 (FE254_04010) - 911275..911823 (-) 549 WP_273257465.1 MOSC domain-containing protein -
  FE254_RS03980 (FE254_04015) - 911820..912449 (-) 630 WP_004425012.1 DUF1780 domain-containing protein -
  FE254_RS03985 (FE254_04020) - 912648..913337 (+) 690 WP_273257463.1 energy-coupling factor ABC transporter permease -
  FE254_RS03990 (FE254_04025) - 913444..913659 (+) 216 WP_112211225.1 hypothetical protein -
  FE254_RS03995 (FE254_04030) yacG 913773..913967 (-) 195 WP_004425006.1 DNA gyrase inhibitor YacG -
  FE254_RS04000 (FE254_04035) coaE 913964..914572 (-) 609 WP_059390436.1 dephospho-CoA kinase -
  FE254_RS04005 (FE254_04040) pilD 914792..915661 (-) 870 WP_273257458.1 A24 family peptidase Machinery gene
  FE254_RS04010 (FE254_04045) pilC 915665..916882 (-) 1218 WP_186348772.1 type II secretion system F family protein Machinery gene
  FE254_RS04015 (FE254_04050) pilB 916885..918588 (-) 1704 WP_273257455.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FE254_RS04020 (FE254_04055) pilE 918811..919248 (+) 438 WP_273257453.1 pilin Machinery gene
  FE254_RS04025 (FE254_04060) - 919314..921251 (+) 1938 WP_273257451.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44008.73 Da        Isoelectric Point: 9.6469

>NTDB_id=363545 FE254_RS04010 WP_186348772.1 915665..916882(-) (pilC) [Pseudomonas guguanensis strain HMFL31]
MAEKALKTSVFTWEGKNKSGAVVKGELSGQNPSLVKAQLRKQGINPTKVRKKGVSLFSAGKKIKPMDIALFTRQMATMMK
AGVPLLQSFDIISEGFDNPNMRKLVDEVKQEVAAGNSFAASLRKKPEYFDELYCNLVESGEQAGALENLLDRIATYKEKT
EALKAKIKKAMNYPIAVVVVAIIVSAILLIKVVPQFEEVFANFGAELPAFTLMVIGISQFLQEWWFIFLIAIIAAAFAFK
EAMKRSQKLRDSVDRGVLKLPIVGDILYKSAVARFARTLSTTFAAGVPLVDALDSVSGATGNVVFKNATNKIKADVSTGM
QLNFSMRTTGTFPSMAVQMTAIGEESGSLDEMLDKVASFYEAEVDNMVDGLTSLMEPIIMSVLGVLVGGLIIAMYLPIFQ
LGAVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=363545 FE254_RS04010 WP_186348772.1 915665..916882(-) (pilC) [Pseudomonas guguanensis strain HMFL31]
ATGGCGGAAAAAGCTCTTAAAACCAGTGTGTTCACCTGGGAAGGCAAGAACAAAAGCGGTGCCGTCGTCAAGGGGGAGCT
CAGCGGGCAGAACCCGTCCTTGGTCAAGGCGCAACTGCGCAAACAGGGGATCAATCCGACCAAAGTACGCAAAAAGGGGG
TGTCGCTCTTCAGCGCAGGCAAGAAGATCAAACCCATGGATATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAA
GCTGGCGTGCCACTGCTGCAGTCCTTCGACATCATCTCAGAGGGCTTCGACAACCCCAACATGCGCAAGCTGGTCGACGA
GGTGAAGCAGGAGGTGGCTGCTGGTAACAGTTTTGCCGCCTCGCTGCGCAAGAAGCCGGAATACTTCGATGAACTGTACT
GCAACCTGGTCGAATCCGGCGAACAGGCCGGCGCGCTGGAAAACCTGCTGGATCGAATCGCCACCTACAAGGAAAAGACC
GAGGCGCTCAAAGCCAAGATCAAAAAAGCGATGAATTACCCTATCGCTGTTGTCGTTGTCGCAATTATCGTTTCAGCTAT
TTTGCTGATTAAAGTGGTGCCACAGTTTGAAGAGGTATTTGCTAACTTCGGCGCTGAACTACCTGCCTTTACGCTGATGG
TAATAGGCATATCGCAATTTCTTCAGGAGTGGTGGTTCATTTTTCTGATTGCAATCATCGCCGCTGCCTTTGCATTCAAA
GAAGCGATGAAGCGCTCGCAGAAACTGCGCGACTCGGTTGACCGTGGCGTACTGAAACTGCCAATCGTCGGCGACATTCT
TTACAAATCTGCGGTAGCCCGCTTCGCACGCACACTATCAACGACTTTTGCCGCTGGCGTGCCACTGGTCGATGCGCTGG
ATTCGGTCTCCGGCGCCACCGGTAATGTGGTGTTCAAGAATGCGACCAACAAGATCAAAGCAGATGTATCTACCGGCATG
CAGCTTAACTTCTCAATGCGCACCACCGGCACCTTCCCCTCCATGGCCGTGCAAATGACCGCTATCGGCGAAGAGTCTGG
CTCGCTGGATGAGATGCTCGACAAGGTGGCCAGCTTCTATGAAGCAGAAGTGGACAATATGGTCGACGGCCTAACCAGCC
TGATGGAGCCGATCATCATGTCCGTACTGGGCGTGCTGGTCGGTGGCCTTATCATCGCCATGTACCTGCCTATCTTCCAG
TTGGGCGCAGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

82.469

100

0.825
  pilC Acinetobacter baylyi ADP1

59.852

100

0.6
  pilC Acinetobacter baumannii D1279779

59.506

100

0.595
  pilC Legionella pneumophila strain ERS1305867

53.283

97.778

0.521
  pilG Neisseria gonorrhoeae MS11

45.522

99.259

0.452
  pilG Neisseria meningitidis 44/76-A

45.522

99.259

0.452
  pilC Vibrio cholerae strain A1552

41.975

100

0.42
  pilC Vibrio campbellii strain DS40M4

40.657

97.778

0.398

Multiple sequence alignment