Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TCK1_RS25320 Genome accession   NZ_CP040324
Coordinates   5560452..5561450 (-) Length   332 a.a.
NCBI ID   WP_019097366.1    Uniprot ID   A0AAE6RFX8
Organism   Pseudomonas monteilii strain TCU-CK1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5555452..5566450
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TCK1_RS25300 (TCK1_5068) argS 5556594..5558330 (+) 1737 WP_025752264.1 arginine--tRNA ligase -
  TCK1_RS25305 (TCK1_5069) - 5558332..5559030 (+) 699 WP_016712504.1 SPOR domain-containing protein -
  TCK1_RS25310 (TCK1_5070) - 5559115..5559531 (-) 417 WP_016712505.1 NINE protein -
  TCK1_RS25315 (TCK1_5071) - 5559698..5560324 (+) 627 WP_016712506.1 C40 family peptidase -
  TCK1_RS25320 (TCK1_5072) pilT 5560452..5561450 (-) 999 WP_019097366.1 type IV pilus twitching motility protein PilT Machinery gene
  TCK1_RS25325 (TCK1_5073) - 5561516..5562202 (+) 687 WP_019097367.1 YggS family pyridoxal phosphate-dependent enzyme -
  TCK1_RS25330 (TCK1_5074) proC 5562241..5563059 (+) 819 WP_025752259.1 pyrroline-5-carboxylate reductase -
  TCK1_RS25335 (TCK1_5075) - 5563070..5563660 (+) 591 WP_025752257.1 YggT family protein -
  TCK1_RS25340 (TCK1_5076) metX 5563749..5564888 (+) 1140 WP_019097209.1 homoserine O-succinyltransferase MetX -
  TCK1_RS25345 (TCK1_5077) metW 5564896..5565516 (+) 621 WP_003249106.1 methionine biosynthesis protein MetW -
  TCK1_RS25350 (TCK1_5078) - 5565539..5565973 (+) 435 WP_019097208.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 36036.66 Da        Isoelectric Point: 5.9334

>NTDB_id=363267 TCK1_RS25320 WP_019097366.1 5560452..5561450(-) (pilT) [Pseudomonas monteilii strain TCU-CK1]
MDVTDLLARAVEAGASDLHLAAGQIPMLRLDGELQRMNLPPLVAADLAEGMALLMTEQQRRQWAEGDELDLALALPTLGR
FRLNLFRQLNGPGATFRLIPGRIATLDELDLGDVYQAVAQCHDGLVLVGGPTGSGKSSTLASLLDQLNRERALHIITLED
PVEVIHSSQRSLVNQREIGRHSGGFAQGLRSALRQDPDVIMIGELRDLETVRLALRAAETGHLVLATVHTRSAVNSIDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVRRVDGGRVAAREVLVATTAVRNLVREGRMAQLCSVMQAGAAEGMRTMEGAV
RELREMGLIKDL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=363267 TCK1_RS25320 WP_019097366.1 5560452..5561450(-) (pilT) [Pseudomonas monteilii strain TCU-CK1]
ATGGATGTGACCGACCTGTTGGCCCGAGCCGTGGAGGCGGGGGCTTCGGACCTGCACCTGGCGGCGGGCCAGATACCGAT
GCTGCGCCTGGACGGTGAATTGCAGCGCATGAACTTGCCACCGCTGGTGGCGGCAGACCTGGCCGAGGGCATGGCGCTCT
TGATGACAGAGCAGCAAAGGCGGCAATGGGCCGAGGGCGATGAACTGGACTTGGCGCTGGCATTGCCCACGCTCGGCCGT
TTTCGGCTGAACCTGTTTCGCCAGCTGAATGGCCCTGGAGCAACCTTTCGGCTGATCCCCGGGCGGATCGCCACGCTGGA
TGAACTGGACCTGGGGGATGTGTATCAAGCTGTTGCGCAATGCCATGATGGTTTGGTCTTGGTGGGTGGGCCGACCGGCA
GTGGCAAGTCCAGCACCCTGGCGTCGCTGCTTGACCAGCTGAACCGGGAGCGGGCGCTGCACATCATTACCCTGGAAGAC
CCGGTCGAAGTTATCCACAGCAGCCAACGCAGCCTGGTCAATCAGCGTGAGATTGGGCGCCACAGCGGCGGCTTTGCCCA
AGGGCTGCGCAGTGCCCTGCGACAGGACCCGGATGTGATCATGATTGGCGAATTGCGCGACCTGGAGACCGTTCGTCTGG
CCTTGCGCGCGGCAGAGACCGGGCACCTGGTGCTGGCGACGGTGCATACGCGTTCGGCCGTAAACAGCATCGATCGGTTG
GTGGAGGTGTTTGCTGCCGAGGAAAAGCCGTTGGTGCGCGCGATGCTGGCCGAGTCGCTGCGCCTGGTGGTGGCGCAGGT
GCTGGTCAGGCGCGTAGATGGCGGGCGGGTTGCAGCGCGGGAAGTGCTGGTGGCGACGACGGCGGTGCGTAACCTGGTGC
GTGAGGGGCGGATGGCGCAGTTGTGTTCGGTGATGCAGGCGGGTGCTGCAGAAGGGATGCGGACGATGGAGGGGGCCGTG
CGGGAGCTGAGAGAGATGGGTCTGATCAAAGATCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.672

99.096

0.512
  pilT Acinetobacter nosocomialis M2

51.064

99.096

0.506
  pilT Acinetobacter baumannii D1279779

51.064

99.096

0.506
  pilT Acinetobacter baumannii strain A118

51.064

99.096

0.506
  pilT Acinetobacter baylyi ADP1

50.909

99.398

0.506
  pilT Pseudomonas stutzeri DSM 10701

50.456

99.096

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.544

99.096

0.491
  pilT Vibrio cholerae strain A1552

49.544

99.096

0.491
  pilT Legionella pneumophila strain Lp02

49.24

99.096

0.488
  pilT Legionella pneumophila strain ERS1305867

49.24

99.096

0.488
  pilT Neisseria meningitidis 8013

48.485

99.398

0.482
  pilT Neisseria gonorrhoeae MS11

48.182

99.398

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.742

95.783

0.467
  pilU Pseudomonas stutzeri DSM 10701

39.394

99.398

0.392

Multiple sequence alignment