Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FD724_RS00565 Genome accession   NZ_CP040281
Coordinates   157714..158841 (-) Length   375 a.a.
NCBI ID   WP_114080205.1    Uniprot ID   A0A6P1KRE8
Organism   Nostoc sp. C057     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 152714..163841
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FD724_RS00540 (FD724_00550) - 153517..153861 (-) 345 WP_256874410.1 type II toxin-antitoxin system PemK/MazF family toxin -
  FD724_RS00545 (FD724_00555) - 153834..154094 (-) 261 WP_179071228.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  FD724_RS00550 (FD724_00560) - 154565..155569 (+) 1005 WP_179071229.1 saccharopine dehydrogenase-like oxidoreductase -
  FD724_RS00555 (FD724_00565) - 155697..156257 (-) 561 WP_179071230.1 hypothetical protein -
  FD724_RS00560 (FD724_00570) - 156386..157609 (-) 1224 WP_179071231.1 type II secretion system F family protein -
  FD724_RS00565 (FD724_00575) pilT 157714..158841 (-) 1128 WP_114080205.1 type IV pilus twitching motility protein PilT Machinery gene
  FD724_RS00570 (FD724_00580) pilB/pilB1 158956..160962 (-) 2007 WP_179071232.1 GspE/PulE family protein Machinery gene
  FD724_RS00575 (FD724_00585) grpE 161420..162169 (+) 750 WP_114080203.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41042.25 Da        Isoelectric Point: 7.4775

>NTDB_id=363008 FD724_RS00565 WP_114080205.1 157714..158841(-) (pilT) [Nostoc sp. C057]
MEMMIEDLMEQLIELGGSDMHLSAGLPPYFRISGKLTPIGEHILTADQCQRLIFSMLNNTQRKTLEQNWELDCSYGVKGL
ARFRVNVYKERGSYAACLRALSSKIPNFEKLGLPDIVREMTDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIVLVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPHERQTQVRVQLSNSLVAVFSQTLVSKKNPKPGEYGRVMAQEILIVTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADFYKSGTISFEAAMSKTSKPDEIQRLIGNSTPQAGAGAKSGAAAKAH

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=363008 FD724_RS00565 WP_114080205.1 157714..158841(-) (pilT) [Nostoc sp. C057]
ATGGAAATGATGATTGAAGATTTGATGGAACAGTTGATTGAACTGGGTGGCTCGGATATGCATTTATCCGCAGGTTTGCC
TCCCTACTTCCGCATCAGTGGCAAACTCACTCCCATCGGTGAGCATATATTGACAGCCGATCAATGTCAAAGGCTGATTT
TTAGTATGCTCAACAACACCCAGCGTAAAACCTTAGAGCAAAACTGGGAACTGGATTGTTCTTATGGGGTTAAAGGTTTG
GCACGTTTCCGGGTCAATGTTTATAAAGAACGTGGTTCTTATGCTGCTTGCTTACGGGCATTAAGTTCTAAGATTCCTAA
CTTTGAAAAATTAGGTCTGCCAGATATCGTAAGGGAAATGACAGATAAGCCCAGAGGACTGATTCTGGTGACAGGGCCTA
CAGGTTCCGGGAAGACAACTACCCTAGCAGCGATGATCGACTTGATTAACCGCACTAAAGCAGAGCATATTTTAACGGTG
GAAGACCCAATTGAATTTGTCTACGAACCAATTAAAAGCTTGGTTCACCAACGACAACTGGGTGAAGATACTAAGAGCTT
TGCTAACGCTTTGAAAGCAGCTTTGCGGGAAGATCCAGATATTGTTCTGGTGGGAGAAATGCGCGATTTGGAAACGATTT
CTTTGGCGATTTCCGCAGCAGAAACAGGACACTTGGTATTTGGTACTCTGCACACCAGTTCTGCCGCCCAGACAGTTGAC
CGGATTATTGACGTTTTCCCCCATGAAAGACAAACCCAGGTGCGGGTGCAGTTATCTAACTCGTTAGTGGCAGTATTTAG
CCAAACGTTGGTGTCCAAGAAAAACCCGAAACCTGGTGAATATGGTCGGGTGATGGCTCAAGAAATTCTGATTGTCACTC
CTGCTATTTCCAACTTGATTCGAGAAGGCAAAACATCTCAAATTTACTCAGCTATTCAAACTGGCGGCAAATTGGGGATG
CAAACTCTGGAGAAAGTTTTAGCTGATTTTTACAAATCAGGAACGATTTCCTTTGAAGCCGCGATGTCTAAAACTTCTAA
GCCAGATGAAATCCAACGTCTCATCGGGAATTCTACACCGCAAGCAGGCGCAGGTGCAAAATCCGGTGCGGCTGCCAAAG
CGCATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P1KRE8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.467

92.533

0.504
  pilT Pseudomonas stutzeri DSM 10701

53.868

93.067

0.501
  pilT Legionella pneumophila strain ERS1305867

52.738

92.533

0.488
  pilT Acinetobacter baylyi ADP1

52.738

92.533

0.488
  pilT Legionella pneumophila strain Lp02

52.738

92.533

0.488
  pilT Acinetobacter nosocomialis M2

51.873

92.533

0.48
  pilT Acinetobacter baumannii D1279779

51.873

92.533

0.48
  pilT Acinetobacter baumannii strain A118

51.873

92.533

0.48
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.411

97.867

0.464
  pilT Neisseria gonorrhoeae MS11

50.737

90.4

0.459
  pilT Neisseria meningitidis 8013

50.442

90.4

0.456
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.415

91.2

0.451
  pilT Vibrio cholerae strain A1552

49.415

91.2

0.451

Multiple sequence alignment