Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   FD724_RS00570 Genome accession   NZ_CP040281
Coordinates   158956..160962 (-) Length   668 a.a.
NCBI ID   WP_179071232.1    Uniprot ID   -
Organism   Nostoc sp. C057     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 153956..165962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FD724_RS00550 (FD724_00560) - 154565..155569 (+) 1005 WP_179071229.1 saccharopine dehydrogenase-like oxidoreductase -
  FD724_RS00555 (FD724_00565) - 155697..156257 (-) 561 WP_179071230.1 hypothetical protein -
  FD724_RS00560 (FD724_00570) - 156386..157609 (-) 1224 WP_179071231.1 type II secretion system F family protein -
  FD724_RS00565 (FD724_00575) pilT 157714..158841 (-) 1128 WP_114080205.1 type IV pilus twitching motility protein PilT Machinery gene
  FD724_RS00570 (FD724_00580) pilB/pilB1 158956..160962 (-) 2007 WP_179071232.1 GspE/PulE family protein Machinery gene
  FD724_RS00575 (FD724_00585) grpE 161420..162169 (+) 750 WP_114080203.1 nucleotide exchange factor GrpE -
  FD724_RS00580 (FD724_00590) dnaK 162396..164354 (+) 1959 WP_114080202.1 molecular chaperone DnaK -

Sequence

Protein


Download         Length: 668 a.a.        Molecular weight: 74565.44 Da        Isoelectric Point: 5.1746

>NTDB_id=363009 FD724_RS00570 WP_179071232.1 158956..160962(-) (pilB/pilB1) [Nostoc sp. C057]
MTYSSPQRRSTALTTRTEFSPFGNKLVQSGYVNTEQMRQALIESRKSGRPLTEVLESITGQQLSPELLRQYKKQQLFELK
ILYGVEFLDPEVNSIGNTMMGNLIETLIPVDICRRHRLVPLSKHEDQTPPSVLVAMVAPDNLEASDDLNRILRPQGLALQ
RMVITQEDYQQLINQYLDEMAVRQKHLEQEKFTDINQDLENLGNLDLADAPEEMEADLGAAMKGAEDAPVINLVNRILAK
ALHEGVSDIHIEPQEENLRIRFRKDGVLKEAFDPLPKKIIPAVTARFKIIANLDIAERRLPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLDKLITDPETLHIVQDMVSRPFGLILVTGPTGSGKTTSLYSALSEKNDPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCSECRIPYTPTTEELARYGLSASSDVAITFYKANTLTLDAIAEAKGKNQLCSKCNG
VGYKGRCGVYEVMRVTETLQTLINEDAPTERIKEVAIEEGMKTLLAYSLDLVREGSTTLEEVERVTFTDTGLEAELKAKR
KTGLTCRSCDATLKQEWLDCPYCMTSRF

Nucleotide


Download         Length: 2007 bp        

>NTDB_id=363009 FD724_RS00570 WP_179071232.1 158956..160962(-) (pilB/pilB1) [Nostoc sp. C057]
ATGACTTACTCGTCACCACAACGGCGCAGTACCGCTCTAACTACCAGAACAGAGTTTTCGCCCTTTGGCAACAAGCTAGT
GCAATCTGGCTATGTCAATACCGAACAGATGAGGCAGGCACTAATTGAAAGCCGCAAATCTGGCCGACCCTTAACGGAAG
TACTAGAGTCAATTACTGGGCAACAACTATCACCTGAGTTGCTCAGGCAATACAAAAAACAGCAGCTATTTGAACTTAAA
ATACTATACGGTGTTGAATTCCTCGATCCGGAAGTCAACTCCATTGGCAACACGATGATGGGGAACCTGATTGAAACCCT
CATCCCAGTGGATATCTGCCGTCGCCATCGTTTAGTACCACTATCGAAACACGAAGACCAAACCCCGCCCTCAGTTTTAG
TGGCGATGGTCGCTCCGGATAATCTAGAGGCTTCTGATGACCTGAACCGCATCTTGCGTCCCCAAGGGCTGGCATTACAG
CGCATGGTGATTACACAGGAAGACTACCAGCAGCTAATCAACCAATATCTGGATGAAATGGCTGTTCGGCAAAAACACCT
GGAACAAGAAAAGTTTACAGATATTAATCAGGATTTAGAAAACCTCGGAAATCTTGATCTTGCAGATGCTCCTGAAGAAA
TGGAGGCTGATTTAGGAGCAGCGATGAAGGGTGCGGAGGATGCCCCAGTCATCAACCTAGTCAACAGAATCCTGGCTAAA
GCCTTACATGAGGGCGTTTCTGACATTCACATCGAACCGCAAGAAGAAAACTTACGCATCCGCTTTCGGAAGGATGGGGT
ACTGAAGGAAGCTTTCGATCCCCTACCGAAAAAAATCATCCCGGCGGTGACAGCCCGATTTAAAATCATCGCCAATCTAG
ACATTGCTGAACGCCGTCTACCCCAAGATGGACGTATCCGCCGGATGTTTGAGGGACGTAAGGTGGACTTCCGGGTGAAT
ACCTTGCCCAGCCGCTACGGGGAAAAGGTGGTACTGCGGATTTTGGATAACTCCTCCACCCAACTGGGATTAGATAAGTT
AATTACCGATCCAGAGACTTTACATATTGTCCAGGATATGGTTAGCCGTCCCTTTGGGCTAATTTTGGTAACTGGGCCAA
CTGGTTCTGGGAAAACAACTTCGCTGTATTCTGCACTATCAGAAAAGAACGATCCCGGAATTAATATCAGTACTGTGGAA
GACCCAATTGAGTACAGTCTTCCAGGGATTACGCAAGTACAGGTGATTCGGGAAAAAGGGCTAGATTTTGCTACTGCTCT
GCGGGCTTTCTTGCGACAAGATCCAGATGTGCTACTGGTGGGTGAAACGCGGGATAAGGAAACGGCAAAAACAGCTATTG
AAGCTGCCTTGACTGGTCACTTAGTATTAACTACTTTACATACCAATGATGCCCCAGGTGCGATCGCTCGTTTGGGAGAA
ATGGGGATTGAGCCTTTCATGGTTTCTAGTTCGCTAATTGGCGTTCTAGCTCAACGTTTGGTGCGGCGTGTATGTTCGGA
ATGTCGTATTCCCTACACTCCTACAACCGAGGAACTGGCTCGTTATGGTCTATCAGCTTCTTCTGATGTCGCAATTACCT
TCTATAAGGCTAACACCTTGACATTAGATGCGATCGCCGAAGCCAAAGGCAAAAATCAGCTTTGCTCAAAATGTAATGGT
GTTGGCTACAAAGGTCGTTGTGGTGTTTATGAAGTCATGCGAGTCACCGAAACCCTGCAAACTCTTATCAACGAAGATGC
ACCCACAGAACGCATCAAGGAAGTGGCAATAGAAGAGGGCATGAAAACTTTGCTGGCTTACAGCTTGGACTTAGTGCGCG
AGGGTTCTACCACTTTAGAAGAAGTGGAACGAGTGACGTTTACTGATACTGGTTTGGAAGCCGAGTTAAAAGCCAAACGC
AAGACTGGCCTTACCTGCCGCAGTTGTGATGCCACATTGAAACAAGAATGGCTGGATTGTCCTTACTGTATGACATCTCG
GTTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(64-181)

Type II/IV secretion system protein (T2SSE)

(236-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.518

100

0.669

Multiple sequence alignment