Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FBB35_RS09885 Genome accession   NZ_CP040094
Coordinates   2285037..2286164 (+) Length   375 a.a.
NCBI ID   WP_174709486.1    Uniprot ID   A0A6N0LP54
Organism   Nostoc sp. TCL240-02     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2280037..2291164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FBB35_RS09875 (FBB35_09885) grpE 2281729..2282478 (-) 750 WP_174709484.1 nucleotide exchange factor GrpE -
  FBB35_RS09880 (FBB35_09890) pilB/pilB1 2282930..2284936 (+) 2007 WP_174709485.1 GspE/PulE family protein Machinery gene
  FBB35_RS09885 (FBB35_09895) pilT 2285037..2286164 (+) 1128 WP_174709486.1 type IV pilus twitching motility protein PilT Machinery gene
  FBB35_RS09890 (FBB35_09900) - 2286280..2287503 (+) 1224 WP_174709487.1 type II secretion system F family protein -
  FBB35_RS09895 (FBB35_09905) - 2287625..2288185 (+) 561 WP_174709488.1 hypothetical protein -
  FBB35_RS09900 (FBB35_09910) - 2288319..2289323 (-) 1005 WP_174709489.1 saccharopine dehydrogenase-like oxidoreductase -
  FBB35_RS09905 (FBB35_09915) - 2289920..2290138 (-) 219 Protein_1998 DUF2442 domain-containing protein -
  FBB35_RS35450 (FBB35_09920) - 2290161..2290340 (-) 180 Protein_1999 DUF4160 domain-containing protein -
  FBB35_RS09915 (FBB35_09925) - 2290730..2290939 (+) 210 WP_163928123.1 DUF2997 domain-containing protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41080.34 Da        Isoelectric Point: 7.4775

>NTDB_id=362028 FBB35_RS09885 WP_174709486.1 2285037..2286164(+) (pilT) [Nostoc sp. TCL240-02]
MEMMIEDLMEQLIELGGSDMHLSAGLPPYFRISGKLTPIGEHVLTADQCQRLIFSMLNNTQRKTLEQNWELDCSYGVKGL
ARFRVNVYKERGSYAACLRALSSKIPNFEKLGLPDIVREMTDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIVLVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPHERQTQVRVQLSNSLVAVFSQTLVSKKNPKPGEYGRVMAQEILIVTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADFYKSGTISFEAAMSKTSKPDEIQRLIGNSTPQAAAGAKPGAAAKVH

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=362028 FBB35_RS09885 WP_174709486.1 2285037..2286164(+) (pilT) [Nostoc sp. TCL240-02]
ATGGAAATGATGATTGAAGATTTGATGGAACAGTTGATTGAACTGGGTGGCTCGGATATGCATTTATCCGCAGGTTTGCC
TCCCTACTTCCGCATCAGTGGCAAACTCACTCCCATCGGTGAGCATGTATTGACAGCCGATCAATGTCAAAGGCTGATTT
TTAGTATGCTCAACAACACCCAGCGTAAAACCTTAGAGCAAAACTGGGAACTGGATTGTTCTTATGGGGTTAAAGGTTTA
GCACGTTTTCGGGTCAATGTTTATAAAGAACGTGGTTCTTATGCTGCTTGCTTACGGGCATTAAGTTCTAAGATTCCTAA
CTTTGAAAAATTAGGTCTGCCAGATATCGTAAGGGAAATGACAGATAAGCCCAGAGGACTAATTCTGGTGACAGGCCCTA
CAGGTTCCGGTAAGACAACTACCCTAGCGGCGATGATCGACTTGATTAACCGCACTAAAGCAGAGCATATTTTAACGGTG
GAAGACCCAATTGAATTTGTCTACGAACCAATTAAAAGTTTAGTTCACCAACGACAACTGGGTGAAGATACTAAGAGCTT
TGCTAATGCTTTGAAAGCAGCTTTGCGGGAAGATCCAGATATTGTTCTGGTGGGAGAAATGCGCGATTTGGAAACGATTT
CTTTGGCGATTTCCGCAGCAGAAACAGGACACTTGGTATTCGGTACTCTCCACACCAGTTCTGCCGCCCAGACAGTTGAC
CGGATTATCGACGTTTTCCCCCATGAAAGACAAACCCAGGTGCGGGTACAGTTATCTAACTCGTTAGTGGCAGTATTTAG
CCAAACCTTGGTGTCCAAGAAAAACCCTAAACCTGGTGAATATGGTCGGGTGATGGCTCAAGAAATTCTGATTGTCACTC
CTGCTATTTCCAACTTGATTCGAGAAGGCAAAACATCTCAAATTTACTCAGCTATTCAAACTGGCGGAAAATTGGGGATG
CAAACTCTGGAGAAAGTTTTAGCTGATTTTTACAAATCAGGAACGATTTCCTTTGAAGCCGCGATGTCTAAAACTTCTAA
GCCAGATGAAATCCAACGTCTCATTGGGAATTCTACACCGCAAGCAGCCGCAGGTGCAAAACCCGGTGCAGCGGCCAAAG
TGCATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N0LP54

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.467

92.533

0.504
  pilT Pseudomonas stutzeri DSM 10701

53.868

93.067

0.501
  pilT Acinetobacter baylyi ADP1

52.738

92.533

0.488
  pilT Legionella pneumophila strain Lp02

52.738

92.533

0.488
  pilT Legionella pneumophila strain ERS1305867

52.738

92.533

0.488
  pilT Acinetobacter baumannii strain A118

51.873

92.533

0.48
  pilT Acinetobacter baumannii D1279779

51.873

92.533

0.48
  pilT Acinetobacter nosocomialis M2

51.873

92.533

0.48
  pilT Neisseria gonorrhoeae MS11

50.737

90.4

0.459
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.169

94.667

0.456
  pilT Neisseria meningitidis 8013

50.442

90.4

0.456
  pilT Vibrio cholerae strain A1552

49.415

91.2

0.451
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.415

91.2

0.451

Multiple sequence alignment