Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   FBB35_RS09880 Genome accession   NZ_CP040094
Coordinates   2282930..2284936 (+) Length   668 a.a.
NCBI ID   WP_174709485.1    Uniprot ID   A0A6N0LP56
Organism   Nostoc sp. TCL240-02     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2277930..2289936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FBB35_RS09870 (FBB35_09880) dnaK 2279545..2281503 (-) 1959 WP_174709483.1 molecular chaperone DnaK -
  FBB35_RS09875 (FBB35_09885) grpE 2281729..2282478 (-) 750 WP_174709484.1 nucleotide exchange factor GrpE -
  FBB35_RS09880 (FBB35_09890) pilB/pilB1 2282930..2284936 (+) 2007 WP_174709485.1 GspE/PulE family protein Machinery gene
  FBB35_RS09885 (FBB35_09895) pilT 2285037..2286164 (+) 1128 WP_174709486.1 type IV pilus twitching motility protein PilT Machinery gene
  FBB35_RS09890 (FBB35_09900) - 2286280..2287503 (+) 1224 WP_174709487.1 type II secretion system F family protein -
  FBB35_RS09895 (FBB35_09905) - 2287625..2288185 (+) 561 WP_174709488.1 hypothetical protein -
  FBB35_RS09900 (FBB35_09910) - 2288319..2289323 (-) 1005 WP_174709489.1 saccharopine dehydrogenase-like oxidoreductase -

Sequence

Protein


Download         Length: 668 a.a.        Molecular weight: 74551.36 Da        Isoelectric Point: 5.1727

>NTDB_id=362027 FBB35_RS09880 WP_174709485.1 2282930..2284936(+) (pilB/pilB1) [Nostoc sp. TCL240-02]
MTYSSPQRRSTALTTRTEFSPFGNKLVQSGYVNTEQMRQALIESRKSGRPLTEVLESITGQQLSPELLRQYKKQQLFELK
ILYGVEFLDPEVNSIGNTMMGNLIETLIPVDICRRHRLVPLSKHEDQTPPSVLVAMVAPDNLEASDDLNRILRPQGLALQ
RMVITQEDYQQLINQYLDEMAVKQKHLEQEKFTDINQDLENLGNLDISDAPEEMEADLGAAMKGAEDAPVINLVNRILAK
ALHEGVSDIHIEPQEENLRIRFRKDGVLREAFDPLPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRLFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLDKLITDPETLHIVQDMVSRPFGLILVTGPTGSGKTTSLYSALSEKNDPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCSECRIPYTPTTEELARYGLSASSDVGVTFYKANTLTLDAIADAKAKNQLCSKCNG
VGYKGRCGVYEVMRVTETLQTLINEDAPTERIKEVAIEEGMKTLLAYSLDLVREGSTTLEEVERVTFTDTGLEAELKAKR
KTGLTCRSCDATLKQEWLDCPYCMTSRF

Nucleotide


Download         Length: 2007 bp        

>NTDB_id=362027 FBB35_RS09880 WP_174709485.1 2282930..2284936(+) (pilB/pilB1) [Nostoc sp. TCL240-02]
ATGACTTACTCGTCACCACAACGGCGCAGTACCGCTCTAACTACCAGAACAGAGTTTTCGCCCTTTGGCAACAAGCTAGT
GCAATCTGGCTATGTCAATACCGAACAGATGAGGCAGGCACTAATTGAAAGCCGCAAATCTGGCCGACCCTTAACGGAAG
TACTAGAGTCAATTACTGGGCAACAACTATCACCTGAGTTGCTCAGGCAATACAAAAAACAGCAGCTATTTGAACTTAAA
ATACTATACGGTGTTGAATTTCTAGATCCGGAAGTCAACTCCATTGGCAACACGATGATGGGGAACCTGATTGAAACCCT
CATCCCAGTAGATATCTGCCGTCGCCATCGTTTAGTACCACTGTCGAAACACGAAGACCAAACCCCGCCCTCAGTTTTAG
TGGCGATGGTCGCTCCAGATAATCTAGAGGCTTCCGATGACCTGAACCGCATCTTGCGCCCCCAAGGTTTGGCATTGCAG
CGCATGGTGATTACACAGGAAGACTACCAGCAGCTAATCAACCAATATCTGGATGAAATGGCTGTTAAGCAAAAGCATCT
GGAACAAGAAAAGTTTACAGATATTAATCAGGATTTAGAAAACCTTGGAAATCTCGATATTTCGGATGCTCCTGAAGAAA
TGGAGGCCGATCTAGGAGCAGCGATGAAGGGTGCAGAGGATGCTCCAGTCATCAACCTAGTGAACAGAATCCTGGCTAAA
GCCTTGCATGAGGGCGTTTCTGACATTCATATCGAACCACAAGAAGAAAACTTACGCATTCGCTTTCGGAAGGATGGGGT
ACTCCGTGAAGCTTTCGATCCCCTACCGAAAAAAATCATCCCGGCGGTGACAGCCCGATTTAAAATCATCTCGAATCTAG
ACATTGCCGAACGCCGTTTACCCCAAGATGGACGCATCCGGCGGCTGTTTGAGGGACGTAAGGTGGATTTCCGCGTGAAT
ACCTTGCCCAGTCGCTACGGGGAAAAGGTGGTGCTGCGGATTTTGGATAACTCTTCAACACAACTGGGATTAGATAAGTT
AATTACCGATCCAGAGACTTTACATATTGTCCAGGATATGGTGAGCCGCCCCTTTGGTCTAATTTTGGTGACTGGGCCAA
CTGGTTCTGGGAAAACAACCTCGCTGTATTCTGCACTATCAGAAAAGAATGATCCCGGAATTAATATCAGTACTGTGGAA
GACCCAATTGAGTACAGTCTTCCAGGGATTACTCAAGTACAGGTGATTCGGGAAAAAGGGCTGGATTTTGCGACTGCGTT
GCGGGCTTTCTTGCGGCAAGATCCAGATGTGCTACTGGTGGGTGAAACGCGGGATAAGGAAACGGCAAAAACAGCAATTG
AGGCTGCCTTGACTGGTCACTTGGTATTAACTACCTTACACACCAATGATGCCCCAGGTGCGATCGCTCGTTTGGGAGAA
ATGGGCATTGAGCCTTTCATGGTTTCTAGTTCTTTAATTGGCGTTCTAGCTCAACGTTTGGTGCGGCGCGTATGTTCTGA
ATGTCGTATTCCCTACACTCCTACAACCGAGGAACTGGCTCGGTATGGTTTATCAGCTTCTTCTGATGTGGGAGTCACCT
TCTATAAAGCTAACACCTTGACATTAGATGCGATCGCAGATGCCAAAGCCAAAAATCAGCTTTGCTCAAAATGTAATGGT
GTTGGCTACAAAGGTCGTTGTGGTGTTTATGAAGTCATGCGAGTCACCGAAACCCTGCAAACTCTGATCAACGAAGATGC
ACCCACGGAACGCATCAAGGAAGTGGCAATAGAAGAGGGTATGAAAACCTTGCTGGCTTACAGCTTGGACTTAGTGCGCG
AGGGTTCCACCACTTTAGAAGAAGTAGAACGAGTGACGTTTACTGATACTGGTTTGGAAGCCGAGTTAAAAGCCAAACGC
AAGACTGGCCTTACCTGCCGCAGTTGTGATGCCACATTGAAACAAGAATGGCTGGATTGTCCTTATTGTATGACATCTCG
ATTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(64-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N0LP56

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.518

100

0.669
  pilF Thermus thermophilus HB27

39.315

91.766

0.361

Multiple sequence alignment