Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   E5678_RS18035 Genome accession   NZ_CP039252
Coordinates   3941412..3941840 (+) Length   142 a.a.
NCBI ID   WP_281728081.1    Uniprot ID   -
Organism   Hydrogenophaga sp. PAMC20947     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3936412..3946840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5678_RS18015 (E5678_18005) - 3937219..3938022 (+) 804 WP_136179809.1 3',5'-cyclic-nucleotide phosphodiesterase -
  E5678_RS18020 (E5678_18010) pilA2 3938260..3938679 (+) 420 WP_136179810.1 pilin Machinery gene
  E5678_RS18025 (E5678_18015) pilA2 3939295..3939723 (+) 429 WP_281728079.1 pilin Machinery gene
  E5678_RS18030 (E5678_18020) pilA2 3940339..3940767 (+) 429 WP_281728080.1 pilin Machinery gene
  E5678_RS18035 (E5678_18025) pilA2 3941412..3941840 (+) 429 WP_281728081.1 pilin Machinery gene
  E5678_RS18040 (E5678_18030) - 3942175..3942678 (+) 504 WP_136179811.1 hypothetical protein -
  E5678_RS18045 (E5678_18035) moaC 3942830..3943267 (-) 438 WP_247597050.1 cyclic pyranopterin monophosphate synthase MoaC -
  E5678_RS18050 (E5678_18040) - 3943421..3945082 (+) 1662 WP_247596820.1 M48 family metalloprotease -
  E5678_RS18055 (E5678_18045) - 3945065..3945415 (-) 351 WP_136179814.1 phage holin family protein -
  E5678_RS18060 (E5678_18050) - 3945459..3946181 (-) 723 WP_136179815.1 TerC family protein -
  E5678_RS18065 (E5678_18055) - 3946441..3946827 (+) 387 WP_136179816.1 VOC family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14493.87 Da        Isoelectric Point: 9.6850

>NTDB_id=357645 E5678_RS18035 WP_281728081.1 3941412..3941840(+) (pilA2) [Hydrogenophaga sp. PAMC20947]
MNRSMQKMQKGFTLIELMIVVAIIGILAAVALPAYQDYTVRARVSESLVAASAAKIAVAENAANGQTLASGWVAPSATPN
MTSAAIAADGVITVTTTARAGGGTVTFVPDPVLVAGTIPTDRIGWTCTGGTLVAKYRPAACR

Nucleotide


Download         Length: 429 bp        

>NTDB_id=357645 E5678_RS18035 WP_281728081.1 3941412..3941840(+) (pilA2) [Hydrogenophaga sp. PAMC20947]
ATGAATCGTTCTATGCAAAAAATGCAAAAGGGTTTTACCCTCATCGAATTGATGATCGTGGTGGCCATCATCGGTATTCT
GGCTGCTGTGGCTCTGCCCGCTTACCAGGACTACACCGTGCGTGCTCGCGTGTCTGAATCGTTGGTTGCCGCTTCCGCTG
CCAAAATTGCTGTAGCTGAAAACGCCGCCAACGGGCAAACCCTTGCGTCGGGTTGGGTTGCACCTTCAGCCACTCCTAAT
ATGACATCTGCTGCGATTGCCGCTGATGGTGTGATCACCGTGACGACCACGGCTCGCGCAGGTGGTGGAACGGTTACCTT
TGTACCAGATCCAGTGCTGGTTGCTGGCACCATCCCAACCGATCGCATCGGTTGGACCTGTACCGGTGGCACATTGGTCG
CGAAGTACCGCCCTGCTGCGTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

62.59

97.887

0.613
  pilA2 Legionella pneumophila strain ERS1305867

61.871

97.887

0.606
  pilA Ralstonia pseudosolanacearum GMI1000

47.879

100

0.556
  comP Acinetobacter baylyi ADP1

45.033

100

0.479
  pilE Neisseria gonorrhoeae MS11

36.842

100

0.444
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.958

100

0.423
  pilA/pilA1 Eikenella corrodens VA1

35.152

100

0.408
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.845

100

0.408
  pilA Vibrio parahaemolyticus RIMD 2210633

39.416

96.479

0.38
  pilA Vibrio cholerae strain A1552

33.121

100

0.366
  pilA Vibrio cholerae C6706

33.121

100

0.366
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

33.121

100

0.366

Multiple sequence alignment