Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   E5678_RS18020 Genome accession   NZ_CP039252
Coordinates   3938260..3938679 (+) Length   139 a.a.
NCBI ID   WP_136179810.1    Uniprot ID   A0A4P7RE10
Organism   Hydrogenophaga sp. PAMC20947     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3933260..3943679
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5678_RS18000 (E5678_17990) - 3933377..3934165 (+) 789 WP_136179807.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  E5678_RS18005 (E5678_17995) - 3934214..3934840 (+) 627 WP_136179808.1 FHA domain-containing protein -
  E5678_RS18010 (E5678_18000) - 3934884..3937151 (+) 2268 WP_247596819.1 adenylate/guanylate cyclase domain-containing protein -
  E5678_RS18015 (E5678_18005) - 3937219..3938022 (+) 804 WP_136179809.1 3',5'-cyclic-nucleotide phosphodiesterase -
  E5678_RS18020 (E5678_18010) pilA2 3938260..3938679 (+) 420 WP_136179810.1 pilin Machinery gene
  E5678_RS18025 (E5678_18015) pilA2 3939295..3939723 (+) 429 WP_281728079.1 pilin Machinery gene
  E5678_RS18030 (E5678_18020) pilA2 3940339..3940767 (+) 429 WP_281728080.1 pilin Machinery gene
  E5678_RS18035 (E5678_18025) pilA2 3941412..3941840 (+) 429 WP_281728081.1 pilin Machinery gene
  E5678_RS18040 (E5678_18030) - 3942175..3942678 (+) 504 WP_136179811.1 hypothetical protein -
  E5678_RS18045 (E5678_18035) moaC 3942830..3943267 (-) 438 WP_247597050.1 cyclic pyranopterin monophosphate synthase MoaC -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14063.40 Da        Isoelectric Point: 9.6850

>NTDB_id=357642 E5678_RS18020 WP_136179810.1 3938260..3938679(+) (pilA2) [Hydrogenophaga sp. PAMC20947]
MKRKLQQGFTLIELMIVVAIIGILAAVALPAYQDYTVRARVSESLVAASAAKIAVAENAANGQALASGWIAPAATPNLAS
AAIAADGVITVTTTARAGGGTVTFVPDPVLVAGTIPTDRIGWTCTGGTLVAKYRPAACR

Nucleotide


Download         Length: 420 bp        

>NTDB_id=357642 E5678_RS18020 WP_136179810.1 3938260..3938679(+) (pilA2) [Hydrogenophaga sp. PAMC20947]
ATGAAGCGCAAATTGCAACAAGGTTTCACCCTCATCGAATTGATGATCGTGGTGGCCATCATCGGTATTCTGGCTGCTGT
GGCTCTGCCCGCTTACCAGGACTACACCGTGCGTGCTCGCGTGTCTGAATCGTTGGTTGCCGCTTCCGCTGCCAAAATTG
CTGTAGCTGAAAATGCCGCCAACGGGCAAGCCCTTGCATCGGGCTGGATCGCTCCTGCAGCGACGCCCAACCTGGCCAGC
GCTGCGATTGCCGCTGATGGTGTGATCACCGTGACGACCACGGCTCGCGCAGGTGGTGGAACGGTTACCTTTGTACCAGA
TCCAGTGCTGGTTGCTGGCACCATCCCAACCGATCGCATCGGTTGGACCTGTACCGGTGGCACATTGGTCGCGAAGTACC
GCCCTGCTGCGTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-139)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P7RE10

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

63.309

100

0.633
  pilA2 Legionella pneumophila strain ERS1305867

61.871

100

0.619
  pilA Ralstonia pseudosolanacearum GMI1000

49.383

100

0.576
  comP Acinetobacter baylyi ADP1

45.695

100

0.496
  pilE Neisseria gonorrhoeae MS11

37.736

100

0.432
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.958

100

0.432
  pilA/pilA1 Eikenella corrodens VA1

37.58

100

0.424
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40

100

0.417
  pilA Vibrio parahaemolyticus RIMD 2210633

41.353

95.683

0.396
  pilA Vibrio cholerae strain A1552

35.374

100

0.374
  pilA Vibrio cholerae C6706

35.374

100

0.374
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

35.374

100

0.374

Multiple sequence alignment