Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E5Q08_RS09865 Genome accession   NZ_CP038987
Coordinates   1981302..1982564 (-) Length   420 a.a.
NCBI ID   WP_010888596.1    Uniprot ID   -
Organism   Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain ATCC 13939 substr. R2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1976302..1987564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5Q08_RS09835 - 1976505..1976951 (+) 447 WP_051618937.1 hypothetical protein -
  E5Q08_RS09840 - 1976948..1977949 (+) 1002 WP_010888591.1 ADP-ribosylglycohydrolase family protein -
  E5Q08_RS09845 - 1977946..1978503 (+) 558 WP_010888592.1 nucleotidyltransferase family protein -
  E5Q08_RS09850 - 1978578..1979777 (+) 1200 WP_010888593.1 acetyl-CoA C-acetyltransferase -
  E5Q08_RS09855 - 1979966..1980649 (+) 684 WP_028328057.1 ankyrin repeat domain-containing protein -
  E5Q08_RS09860 - 1980727..1981239 (+) 513 WP_010888595.1 hypothetical protein -
  E5Q08_RS09865 pilT 1981302..1982564 (-) 1263 WP_010888596.1 type IV pilus twitching motility protein PilT Machinery gene
  E5Q08_RS09870 pilB 1982627..1985290 (-) 2664 WP_027479822.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  E5Q08_RS09875 - 1985470..1985994 (-) 525 WP_010888598.1 YqeG family HAD IIIA-type phosphatase -
  E5Q08_RS09880 pgeF 1985991..1986752 (-) 762 WP_010888599.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45066.43 Da        Isoelectric Point: 6.7744

>NTDB_id=356583 E5Q08_RS09865 WP_010888596.1 1981302..1982564(-) (pilT) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain ATCC 13939 substr. R2]
MTMTSGIDITDILRLAADKGASDIILAAGLPPQFKLSGDYDAQGFEPLSASDTRKLMYSMMNERQQRIFEEKRELDFSFA
LGDKARFRVNAFMQRGNVGGVMRLIPTTVRTIAEMGLPQTVIDIANAPRGLVLVTGPTGSGKSTTLASMIDHINTTKKLH
ILTIEDPIEFMHPNKASIINQREVGSDTLSFDDALRAALRQAPDVILVGEMRDYETIKAAVTAAETGHLVMGTLHTNSAP
ESIDRIVDVFPEEQQEQIRVQLANNLVAVMTQQLLPRADGQGRVLAYEILLANPAVRALIREGKTFQITSVMQTGAREGM
VTMDAYLAGLFRRHLITYDKGLERAIDPKEFARLANDASAGLGGASMTPAAPPVPAYNSASSSSTGRGGDFGRGAAAPAT
PAAPAAGRSEPGRAGGFGRR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=356583 E5Q08_RS09865 WP_010888596.1 1981302..1982564(-) (pilT) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain ATCC 13939 substr. R2]
ATGACCATGACTTCCGGCATCGACATCACCGATATCCTGCGCCTCGCCGCCGACAAGGGTGCCTCCGACATCATCCTGGC
CGCCGGGCTGCCGCCGCAGTTCAAACTCTCGGGCGACTACGACGCGCAGGGCTTCGAGCCGCTGAGCGCGAGTGACACCC
GCAAGCTGATGTACTCGATGATGAACGAGCGCCAGCAGCGCATCTTCGAGGAAAAGCGCGAACTCGACTTTTCCTTCGCG
CTCGGCGACAAGGCCCGCTTCCGCGTGAACGCCTTCATGCAGCGCGGCAACGTGGGCGGCGTGATGCGCCTCATCCCCAC
CACCGTCCGCACGATTGCCGAGATGGGGCTGCCGCAGACGGTGATCGACATCGCCAACGCGCCGCGCGGGCTGGTGCTCG
TCACCGGGCCGACGGGCTCGGGCAAATCGACCACCCTCGCCTCGATGATCGACCACATCAACACGACCAAGAAGCTGCAC
ATCCTGACCATCGAAGACCCCATCGAGTTCATGCACCCCAACAAGGCGAGCATCATCAACCAGCGCGAGGTCGGCAGCGA
CACCCTGAGCTTCGACGACGCCCTGCGCGCCGCGCTGCGTCAGGCCCCCGACGTGATTCTGGTGGGCGAAATGCGCGACT
ACGAGACCATCAAGGCCGCCGTGACCGCCGCCGAAACCGGGCACCTCGTGATGGGCACGCTGCACACCAACTCCGCGCCC
GAATCCATCGACCGCATCGTGGACGTGTTTCCCGAGGAGCAGCAGGAACAGATCCGGGTGCAGCTCGCCAACAACCTCGT
CGCCGTGATGACCCAGCAACTGCTGCCGCGCGCCGACGGGCAGGGACGCGTGCTGGCCTACGAAATCCTGCTGGCGAACC
CGGCGGTCCGCGCCCTGATCCGCGAGGGCAAAACCTTCCAGATCACCAGCGTGATGCAGACCGGCGCCCGCGAAGGCATG
GTCACGATGGACGCCTACCTCGCCGGGCTGTTCCGCCGCCATCTCATCACCTACGACAAGGGGCTGGAACGCGCCATCGA
CCCCAAGGAATTCGCCCGTCTCGCCAACGACGCTTCGGCGGGCCTCGGCGGCGCCAGCATGACGCCCGCCGCGCCGCCCG
TTCCCGCCTACAACAGCGCTTCGAGCAGCAGCACTGGGCGTGGTGGTGACTTCGGACGCGGCGCGGCGGCCCCAGCGACG
CCCGCTGCCCCTGCCGCAGGCCGCAGCGAACCGGGACGCGCCGGAGGCTTTGGCCGCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

100

99.524

0.995
  pilT Neisseria meningitidis 8013

53.644

81.667

0.438
  pilT Pseudomonas stutzeri DSM 10701

52.464

82.143

0.431
  pilT Pseudomonas aeruginosa PAK

52.464

82.143

0.431
  pilT Neisseria gonorrhoeae MS11

53.392

80.714

0.431
  pilT Acinetobacter baumannii strain A118

52.174

82.143

0.429
  pilT Acinetobacter baumannii D1279779

52.174

82.143

0.429
  pilT Acinetobacter nosocomialis M2

52.174

82.143

0.429
  pilT Acinetobacter baylyi ADP1

51.884

82.143

0.426
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.988

77.857

0.405
  pilT Vibrio cholerae strain A1552

51.988

77.857

0.405
  pilT Legionella pneumophila strain Lp02

49.275

82.143

0.405
  pilT Legionella pneumophila strain ERS1305867

49.275

82.143

0.405

Multiple sequence alignment