Detailed information NTG0356584

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: E5Q08_RS09870
• Genome accession: NZ_CP038987
• Coordinates: 1982627..1985290 (-)
• Length: 887 a.a.
• NCBI ID: WP_027479822.1
• Uniprot ID: -
• Organism: Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain ATCC 13939 substr. R2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1977627..1990290

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E5Q08_RS09845	-	1977946..1978503 (+)	558	WP_010888592.1	nucleotidyltransferase family protein	-
E5Q08_RS09850	-	1978578..1979777 (+)	1200	WP_010888593.1	acetyl-CoA C-acetyltransferase	-
E5Q08_RS09855	-	1979966..1980649 (+)	684	WP_028328057.1	ankyrin repeat domain-containing protein	-
E5Q08_RS09860	-	1980727..1981239 (+)	513	WP_010888595.1	hypothetical protein	-
E5Q08_RS09865	pilT	1981302..1982564 (-)	1263	WP_010888596.1	type IV pilus twitching motility protein PilT	Machinery gene
E5Q08_RS09870	pilB	1982627..1985290 (-)	2664	WP_027479822.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
E5Q08_RS09875	-	1985470..1985994 (-)	525	WP_010888598.1	YqeG family HAD IIIA-type phosphatase	-
E5Q08_RS09880	pgeF	1985991..1986752 (-)	762	WP_010888599.1	peptidoglycan editing factor PgeF	-
E5Q08_RS09885	-	1986846..1987640 (+)	795	WP_010888600.1	enoyl-ACP reductase FabI	-
E5Q08_RS09890	-	1987849..1988487 (-)	639	WP_027479823.1	nitroreductase family protein	-
E5Q08_RS09895	-	1988568..1988927 (+)	360	WP_010888602.1	winged helix-turn-helix transcriptional regulator	-
E5Q08_RS09900	-	1988998..1989483 (-)	486	WP_010888603.1	GreA/GreB family elongation factor	-

Sequence

Protein

• Length: 887 a.a.
• Molecular weight: 97248.06 Da
• Isoelectric Point: 4.8389

>NTDB_id=356584 E5Q08_RS09870 WP_027479822.1 1982627..1985290(-) (pilB) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain ATCC 13939 substr. R2]
MALSIGDRRLGAILLDQGYLGDNDLQRALERHSEVGGRLADVLIDSGMVGEKRIARAIEEALGIPLVNLLAVQPDPAALR
SIRPQTALNLQAFPFALEGDRLRVALVDPLSSFSIETLEDDSGFDIEPYQALREEVMWAIATHYPELGLEIVVPSGASDA
GRTGGKLGERLITHGYITDAQLQVALDAQQQTGEALGATLISQRAITEDQLYEVLAEQEGTTFLPNPSGFHPGEEVLGSM
LRADALRLLAVPVDETEQGVTVLTSDPRKRPDIDALIGRPVQLMLTRPRDIERLIEQFYPQRGRLGEQLVQEGTLSRDQL
REALQVQAREGKVKPLGEVITELGFASPDEVDSALQKQNVGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEFLDPIQNPP
DPKVALMIPEATARRYVVVPVRLQGNSLVVAMKDPRNVFALDDLKLITGKEILPAVMAEKDIIRLIERYFGEKGFEKLNK
ELAERNKTQQSQEADLSVADESAIVQVVDSIIREAALQDASDIHIETTEDAVKVRYRIDGALREQNSFPKGAAQQIMARL
KIMGHLDIAERRVPQDGRVRFKKGSIDLDLRLSTLPTVYGEKAVMRLLQKASNIPELEQLGFSEYNYARYTEIIERPNGI
FLVTGPTGSGKSFTCFSTLKRIAKPEKNTTTIEDPIEYEVPGIVQSQVNNSTGMTFARALRAFLRQDPDIIFVGEIRDQE
TAKIAVEAALTGHMVLATLHTNDAPGAVTRLEEMGIENFNISAAVMGVLAQRLVRRVCSECKQPTNADPEVLRRLGISER
DIRGANLMRGTGCPRCGGTGYKGRMGIHELMVMDDSLRRTIGAGRPAAEIRDVALGESGLRSLRQDGIEKALQGLTTLEE
VLAVTAS

Nucleotide

• Length: 2664 bp

>NTDB_id=356584 E5Q08_RS09870 WP_027479822.1 1982627..1985290(-) (pilB) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain ATCC 13939 substr. R2]
TTGGCTCTTTCGATTGGTGACCGCCGCCTGGGCGCGATTTTGCTGGACCAGGGGTATCTGGGCGACAACGACTTGCAGCG
GGCGCTGGAGCGGCACTCCGAGGTGGGGGGCCGCCTCGCTGACGTGCTGATCGACTCCGGCATGGTGGGCGAAAAACGCA
TCGCCCGCGCCATCGAGGAAGCGCTCGGCATTCCGCTGGTCAACCTGCTGGCGGTGCAGCCCGACCCGGCGGCGCTGCGC
TCGATTCGGCCCCAGACGGCCCTCAACTTGCAGGCGTTTCCCTTCGCGCTCGAAGGTGACCGGCTGCGGGTGGCGCTGGT
GGACCCGCTGTCGAGCTTTTCGATCGAGACGCTTGAAGACGACAGCGGCTTCGACATCGAACCGTATCAGGCGCTGCGCG
AGGAAGTGATGTGGGCCATCGCCACGCACTACCCCGAGCTTGGCCTGGAGATCGTGGTGCCCAGCGGGGCCAGCGACGCC
GGGCGCACCGGAGGCAAGCTCGGTGAGCGGCTGATCACGCACGGCTACATCACCGACGCACAGCTTCAGGTGGCGCTCGA
CGCGCAGCAGCAGACCGGCGAGGCGCTCGGGGCGACCCTGATCTCGCAGCGGGCCATCACCGAAGACCAGCTCTACGAGG
TGCTCGCCGAACAGGAAGGCACCACCTTCCTTCCCAACCCCAGCGGTTTTCACCCCGGCGAGGAAGTCCTCGGCAGCATG
CTGCGCGCCGACGCCCTGCGCCTGCTCGCGGTGCCGGTGGACGAAACCGAGCAGGGCGTGACGGTCCTGACGAGCGACCC
GCGCAAGCGCCCCGACATCGACGCGCTCATCGGGCGCCCAGTGCAGTTGATGCTCACGCGCCCGCGCGACATCGAGCGCC
TGATTGAGCAGTTCTACCCGCAACGGGGCCGCCTCGGCGAGCAACTCGTGCAGGAAGGCACGCTGTCGCGCGACCAGTTG
CGCGAGGCGCTTCAGGTGCAGGCCCGTGAGGGCAAGGTCAAGCCGCTCGGCGAGGTCATCACCGAACTCGGATTCGCCAG
CCCCGACGAGGTGGATTCGGCGCTGCAAAAGCAGAACGTCGGCGGAGGCCGCCTGGAAGACACCCTGGTGCAGTCAGGCA
AGCTCAGCCCCGAGATGCTCGCCCGCTCGCTCGCCGCGCAGCTCGGCTACGAGTTCCTCGACCCCATCCAGAACCCGCCG
GACCCCAAGGTCGCGCTGATGATTCCCGAGGCCACCGCCCGCCGCTACGTAGTGGTGCCGGTCAGGCTCCAGGGCAACTC
GCTCGTCGTCGCCATGAAAGACCCGCGCAACGTGTTTGCGCTCGACGACCTCAAGCTGATTACCGGCAAAGAAATCCTGC
CCGCCGTGATGGCGGAAAAAGACATCATCCGCCTGATCGAGCGCTACTTCGGGGAAAAGGGCTTCGAGAAGCTCAACAAG
GAACTCGCCGAGCGCAACAAGACCCAGCAGTCGCAGGAAGCCGACCTCTCGGTGGCCGACGAGAGCGCCATCGTGCAGGT
GGTGGACTCGATTATCCGCGAGGCCGCGCTGCAAGACGCCTCGGACATTCACATCGAAACCACCGAGGACGCCGTCAAGG
TGCGCTACCGCATCGACGGTGCGCTGCGCGAGCAGAACTCCTTTCCCAAGGGCGCGGCGCAGCAGATCATGGCCCGCCTC
AAGATCATGGGCCACCTCGACATCGCCGAGCGCCGCGTGCCGCAAGACGGGCGCGTGCGCTTCAAGAAGGGCAGCATCGA
CCTCGACCTGCGTCTCTCGACCCTGCCCACCGTGTACGGCGAGAAAGCCGTCATGCGTCTGCTGCAAAAGGCGAGCAACA
TCCCCGAACTCGAGCAGCTCGGCTTTTCCGAGTACAACTACGCCCGCTACACCGAGATTATCGAGCGGCCCAACGGCATT
TTTCTGGTCACCGGGCCGACGGGGTCGGGCAAGTCGTTCACCTGTTTTTCGACCCTCAAGCGCATCGCCAAGCCCGAGAA
AAACACCACGACCATCGAAGACCCCATCGAGTACGAGGTGCCGGGCATCGTGCAGTCGCAGGTGAACAACTCGACCGGGA
TGACCTTTGCCCGCGCCCTGCGCGCCTTCCTGCGTCAGGACCCCGACATCATCTTCGTGGGCGAAATCCGTGACCAGGAA
ACCGCCAAGATTGCGGTGGAAGCGGCGCTCACCGGCCACATGGTGCTCGCCACTCTGCACACCAACGACGCGCCAGGCGC
CGTGACCCGCCTTGAGGAAATGGGCATCGAGAACTTCAACATCTCGGCGGCCGTGATGGGCGTGCTCGCGCAGCGGCTGG
TGCGCCGGGTGTGCAGCGAGTGCAAGCAGCCCACCAACGCCGACCCCGAGGTGCTCCGGCGGCTCGGCATCAGCGAGCGC
GATATTCGCGGCGCGAATCTGATGCGCGGGACCGGCTGTCCCCGCTGCGGCGGCACCGGCTACAAGGGCCGCATGGGGAT
CCACGAGCTGATGGTGATGGACGACTCGCTGCGCCGCACCATCGGGGCCGGGCGGCCCGCCGCCGAAATCCGCGACGTGG
CGCTGGGCGAAAGCGGCCTGCGGAGCCTGCGCCAGGACGGCATCGAAAAGGCCCTGCAAGGCCTGACCACCCTTGAAGAA
GTGCTGGCCGTCACCGCGAGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (511-776)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-472)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (200-303)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	100	100	1
pilF	Thermus thermophilus HB27	56.229	100	0.565