Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DD703_RS03890 Genome accession   NC_012880
Coordinates   882717..883757 (+) Length   346 a.a.
NCBI ID   WP_012764406.1    Uniprot ID   -
Organism   Musicola paradisiaca Ech703     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 877717..888757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DD703_RS03855 (Dd703_0769) - 877955..878083 (-) 129 WP_012764399.1 entericidin A/B family lipoprotein -
  DD703_RS03860 (Dd703_0770) hemW 878403..879539 (-) 1137 WP_012764400.1 radical SAM family heme chaperone HemW -
  DD703_RS03865 (Dd703_0771) rdgB 879532..880125 (-) 594 WP_012764401.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  DD703_RS03870 (Dd703_0772) yggU 880229..880528 (-) 300 WP_012764402.1 DUF167 family protein YggU -
  DD703_RS03875 (Dd703_0773) - 880525..881079 (-) 555 WP_012764403.1 YggT family protein -
  DD703_RS03880 (Dd703_0774) proC 881122..881940 (-) 819 WP_012764404.1 pyrroline-5-carboxylate reductase -
  DD703_RS03885 (Dd703_0775) - 881968..882693 (-) 726 WP_012764405.1 YggS family pyridoxal phosphate-dependent enzyme -
  DD703_RS03890 (Dd703_0776) pilT 882717..883757 (+) 1041 WP_012764406.1 type IV pilus twitching motility protein PilT Machinery gene
  DD703_RS20320 - 883824..884099 (+) 276 WP_153247070.1 hypothetical protein -
  DD703_RS03895 (Dd703_0777) codA 884059..885297 (-) 1239 WP_012764407.1 cytosine deaminase -
  DD703_RS03900 (Dd703_0778) - 885294..886571 (-) 1278 WP_012764408.1 PucR family transcriptional regulator -
  DD703_RS03905 (Dd703_0779) codB 886568..887833 (-) 1266 WP_012764409.1 cytosine permease -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 37503.01 Da        Isoelectric Point: 7.1075

>NTDB_id=34331 DD703_RS03890 WP_012764406.1 882717..883757(+) (pilT) [Musicola paradisiaca Ech703]
MDVSEWVCASVKQNASDLHLCSGHPPVMRIDGQLVSAPHQECVRSASLEDWCRQWLEARHWRALARHGQADCALTLPDGT
RLRLNLFRQRHGLSAALRLIPQTAPDLPMLGAPAVVATLAQRRDGLILVTGATGSGKSTTLAAMVSALNQHDARHILTLE
DPIEYLHVSQRCLIQQREVGQHCDDFASALRAALREDPDVLLLGELRDSATIRLALTAAETGHLVLATLHTRSAVQAVDR
LVDVFPAEEKAFVRAQLAASLQAVVAQRLLPGLRGGRVALFEVLLASAAVSNLIREGKTHQLPGILQTGSATGMQTFEQS
YRQRCREGLLPEDGEVFPALSAIAAP

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=34331 DD703_RS03890 WP_012764406.1 882717..883757(+) (pilT) [Musicola paradisiaca Ech703]
ATGGATGTGAGTGAATGGGTGTGCGCTAGTGTAAAACAGAATGCTTCCGATCTGCACCTGTGCAGCGGTCATCCGCCGGT
AATGCGCATCGACGGACAATTAGTCAGCGCTCCGCATCAGGAGTGTGTACGCAGCGCGTCGTTGGAAGACTGGTGTCGGC
AGTGGCTGGAAGCCAGGCATTGGCGGGCGCTGGCGCGGCACGGCCAGGCGGACTGCGCCTTGACCTTGCCGGACGGCACG
CGGTTGCGGCTCAATCTGTTTCGGCAGCGTCATGGTTTGTCGGCGGCGCTGCGTCTGATCCCGCAGACGGCGCCGGATTT
GCCCATGCTCGGCGCGCCTGCGGTGGTGGCAACGCTGGCGCAACGGCGCGATGGGCTGATTCTGGTCACAGGCGCCACCG
GCAGCGGCAAATCCACCACGCTGGCGGCGATGGTGTCGGCGCTCAACCAGCATGACGCGCGGCACATTCTGACGCTGGAA
GATCCGATCGAGTATCTGCATGTCAGCCAGCGCTGTCTGATTCAACAGCGGGAGGTCGGTCAACACTGCGACGATTTCGC
CAGCGCTCTGCGCGCCGCATTGCGTGAAGACCCGGACGTATTACTGCTGGGAGAGCTACGGGATAGCGCCACTATCCGGT
TGGCATTAACCGCAGCGGAAACCGGCCATCTGGTGTTGGCGACGCTGCATACCCGCAGTGCGGTGCAGGCGGTGGACCGG
CTAGTGGATGTGTTTCCGGCGGAGGAAAAGGCGTTCGTGCGGGCGCAGTTGGCCGCCAGCCTACAGGCGGTGGTGGCGCA
ACGGCTGCTGCCGGGGCTGCGTGGGGGGCGGGTTGCGTTGTTCGAGGTATTGCTGGCCTCTGCGGCGGTGAGCAACCTGA
TCCGCGAAGGGAAAACGCACCAGTTGCCCGGCATACTGCAAACCGGGAGTGCGACGGGTATGCAAACCTTTGAGCAGAGT
TACCGGCAGCGCTGCCGGGAAGGGCTGTTGCCGGAAGATGGCGAGGTTTTCCCGGCGCTGTCGGCAATCGCGGCGCCTTG
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(78-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.148

97.399

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.148

97.399

0.488
  pilT Neisseria meningitidis 8013

47.321

97.11

0.46
  pilT Neisseria gonorrhoeae MS11

47.024

97.11

0.457
  pilT Pseudomonas stutzeri DSM 10701

46.567

96.821

0.451
  pilT Acinetobacter baylyi ADP1

46.386

95.954

0.445
  pilT Pseudomonas aeruginosa PAK

46.386

95.954

0.445
  pilT Legionella pneumophila strain ERS1305867

45.104

97.399

0.439
  pilT Legionella pneumophila strain Lp02

45.104

97.399

0.439
  pilT Acinetobacter nosocomialis M2

45.783

95.954

0.439
  pilT Acinetobacter baumannii D1279779

45.783

95.954

0.439
  pilT Acinetobacter baumannii strain A118

45.783

95.954

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.514

92.197

0.41
  pilU Vibrio cholerae strain A1552

37.609

99.133

0.373

Multiple sequence alignment