Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DMR_RS03165 Genome accession   NC_012796
Coordinates   742981..744054 (-) Length   357 a.a.
NCBI ID   WP_012750236.1    Uniprot ID   C4XIZ6
Organism   Solidesulfovibrio magneticus RS-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 737981..749054
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DMR_RS03150 (DMR_06660) acs 738768..740672 (-) 1905 WP_012750233.1 acetate--CoA ligase -
  DMR_RS03155 (DMR_06670) - 740705..741598 (-) 894 WP_012750234.1 LytR/AlgR family response regulator transcription factor -
  DMR_RS03160 (DMR_06680) - 741726..742871 (-) 1146 WP_012750235.1 type IV pilus twitching motility protein PilT -
  DMR_RS03165 (DMR_06690) pilT 742981..744054 (-) 1074 WP_012750236.1 type IV pilus twitching motility protein PilT Machinery gene
  DMR_RS03175 (DMR_06700) - 744392..744577 (+) 186 WP_006921555.1 helix-turn-helix domain-containing protein -
  DMR_RS22260 (DMR_06710) - 744616..747765 (+) 3150 WP_012750237.1 hypothetical protein -
  DMR_RS03185 (DMR_06720) - 747762..748295 (+) 534 WP_012750238.1 hypothetical protein -
  DMR_RS03190 (DMR_06730) - 748324..748833 (+) 510 WP_012750239.1 hypothetical protein -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 38893.91 Da        Isoelectric Point: 6.0040

>NTDB_id=34283 DMR_RS03165 WP_012750236.1 742981..744054(-) (pilT) [Solidesulfovibrio magneticus RS-1]
MAQLDAFFKMLKDTGASDLHLSSGCQPMLRLNGRLERIKYKVLENEELKTLLYEIAPQRLLAVFEETGDLDFAYAAPGLG
RLRCNYFRQERGVAAAFRAIPETVPTLADLGLPDILGELAMRPKGLVLVTGPTGSGKSTTLAAMIRHALEARRDHIITVE
DPIEFLHASAGCLINQREVGRDTRSFAAALRGALREDPDIILVGEMRDLETIELALEAAETGHLVLSTLHTVSAAKTIDR
VIDVFPGDRQAQIRSALSESLTAVISQTLLRRADAPGRVMAMELLLANTAVRNLIRENKIFQINSVLETAKAQGMRTLDD
SLLELLAAGRIEPADALRNAVNKTRFEGLVPAPAASA

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=34283 DMR_RS03165 WP_012750236.1 742981..744054(-) (pilT) [Solidesulfovibrio magneticus RS-1]
ATGGCCCAGCTCGACGCTTTTTTTAAGATGCTCAAGGATACCGGAGCTTCGGACCTGCACCTCTCCAGCGGCTGCCAGCC
CATGCTGCGCCTCAATGGCCGGCTGGAACGCATCAAGTACAAGGTGCTGGAAAACGAGGAATTAAAGACCCTGCTCTACG
AGATCGCGCCCCAGCGGCTGTTGGCCGTTTTCGAGGAAACCGGCGACCTGGACTTCGCCTACGCCGCCCCGGGGCTGGGC
CGGCTTCGCTGCAACTACTTTCGCCAGGAACGCGGCGTGGCCGCCGCCTTCCGGGCCATCCCGGAAACCGTGCCGACACT
GGCCGACCTCGGTCTGCCGGACATCCTGGGCGAGCTGGCCATGCGCCCCAAGGGACTGGTGCTGGTCACCGGCCCCACCG
GCTCGGGCAAGTCCACGACCCTGGCCGCCATGATCCGCCACGCCCTCGAAGCCCGGCGCGACCACATCATCACCGTGGAA
GACCCCATCGAATTCCTCCACGCCTCGGCCGGGTGCCTGATTAACCAGCGCGAGGTGGGCCGCGACACCCGCTCCTTTGC
CGCCGCCCTGCGCGGGGCGCTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAAATGCGCGACCTCGAAACCATCGAGC
TGGCCCTGGAAGCGGCCGAGACCGGCCATCTGGTGCTGTCCACCCTGCACACCGTCTCGGCCGCCAAGACCATCGACCGG
GTCATCGACGTCTTCCCCGGTGACCGGCAGGCGCAGATCCGCTCGGCCCTGTCGGAATCGCTGACCGCCGTCATCTCCCA
AACGCTGCTGCGCCGGGCCGACGCCCCGGGCCGGGTCATGGCCATGGAGCTGCTTCTGGCCAACACCGCCGTGCGCAACC
TCATCCGCGAAAACAAGATCTTCCAGATCAACTCCGTGCTGGAGACGGCCAAGGCCCAGGGGATGCGCACCCTGGACGAC
TCGCTCCTGGAACTCTTGGCCGCCGGACGCATCGAACCGGCCGACGCCCTGCGAAACGCCGTCAACAAGACCCGGTTCGA
AGGCCTTGTCCCGGCCCCGGCCGCTTCGGCCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C4XIZ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

57.895

90.476

0.524
  pilT Acinetobacter baumannii strain A118

57.895

90.476

0.524
  pilT Acinetobacter nosocomialis M2

57.585

90.476

0.521
  pilT Neisseria meningitidis 8013

56.269

91.597

0.515
  pilT Neisseria gonorrhoeae MS11

55.963

91.597

0.513
  pilT Acinetobacter baylyi ADP1

57.595

88.515

0.51
  pilT Pseudomonas stutzeri DSM 10701

54.819

92.997

0.51
  pilT Legionella pneumophila strain ERS1305867

54.079

92.717

0.501
  pilT Legionella pneumophila strain Lp02

54.079

92.717

0.501
  pilT Pseudomonas aeruginosa PAK

54.908

91.317

0.501
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.774

89.076

0.479
  pilT Vibrio cholerae strain A1552

53.774

89.076

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.706

95.238

0.473
  pilU Acinetobacter baylyi ADP1

39.941

94.678

0.378

Multiple sequence alignment