Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ERL55_RS02980 Genome accession   NZ_CP035376
Coordinates   663386..664504 (-) Length   372 a.a.
NCBI ID   WP_129135103.1    Uniprot ID   -
Organism   Luteimonas sp. YGD11-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 658386..669504
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ERL55_RS02945 coq7 658462..659103 (-) 642 WP_129135099.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -
  ERL55_RS02950 rplM 659324..659752 (+) 429 WP_100324397.1 50S ribosomal protein L13 -
  ERL55_RS02955 rpsI 659756..660148 (+) 393 WP_100324398.1 30S ribosomal protein S9 -
  ERL55_RS02965 - 660605..661348 (+) 744 WP_129135100.1 2OG-Fe dioxygenase family protein -
  ERL55_RS02970 - 661565..662551 (+) 987 WP_129135101.1 fumarylacetoacetate hydrolase family protein -
  ERL55_RS02975 maiA 662639..663295 (+) 657 WP_129135102.1 maleylacetoacetate isomerase -
  ERL55_RS02980 pilU 663386..664504 (-) 1119 WP_129135103.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ERL55_RS02985 - 664596..664976 (+) 381 WP_129135104.1 DUF4398 domain-containing protein -
  ERL55_RS02990 - 664979..665569 (+) 591 WP_129135105.1 hypothetical protein -
  ERL55_RS02995 - 665964..666185 (+) 222 WP_129135106.1 YdcH family protein -
  ERL55_RS03000 - 666298..667674 (+) 1377 WP_129135107.1 pyridoxal-phosphate dependent enzyme -
  ERL55_RS03005 - 667922..669100 (+) 1179 WP_129135108.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41517.92 Da        Isoelectric Point: 6.2391

>NTDB_id=339412 ERL55_RS02980 WP_129135103.1 663386..664504(-) (pilU) [Luteimonas sp. YGD11-2]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGKLYPLGNTGLPAGMVKKIAYSLMDEGQVPVFERELELNMALSLQDAGR
FRVNVFKQRGEMGMVIRSIRSTIPSIEELQLPQVLKEIVMAPRGLVLIVGSTGSGKSTTLASMIDHRNSTTSGHILTIED
PIEYLHRHKKSIVNQREVGLDTHTFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFNEAAHKNVLMNLALNLKAVVSQRLVVGRDGRRMPAAEVLINTPMIRDLLRRGQVHEIKQAMEESLEEGMESFDQCL
FRLYKEGRIEMEEALKAADSRDGLALKFRLSQGGDVHDPYADVYSHGEEMLR

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=339412 ERL55_RS02980 WP_129135103.1 663386..664504(-) (pilU) [Luteimonas sp. YGD11-2]
ATGGACATCGGCTATTTCCTGAAGCTGATGACGGAAAAGAACGCGTCGGACATGTTCCTGACCACCGGCGCGCCGGTGTA
CATCAAGGTCGAGGGCAAGCTGTACCCGCTCGGCAACACCGGGCTGCCCGCCGGGATGGTCAAGAAGATCGCCTATTCGT
TGATGGATGAGGGGCAGGTGCCGGTGTTCGAGCGCGAGCTCGAGCTCAACATGGCGCTGTCGCTGCAGGACGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGTGGCGAGATGGGCATGGTGATCCGCTCGATCCGCAGCACCATACCGTCCATCGA
GGAACTGCAGCTGCCGCAGGTGCTCAAGGAGATCGTCATGGCCCCGCGCGGGCTGGTGCTGATCGTCGGCTCCACCGGTT
CCGGCAAGTCGACCACGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCTCCGGGCACATCCTCACCATCGAGGAT
CCGATCGAATACCTGCACCGGCACAAGAAGTCGATCGTCAACCAGCGCGAGGTGGGGCTCGACACCCACACGTTCCACAA
CGCGCTCAAGAATGCGATGCGCGAAGCGCCCGACGTCATCCTGATCGGCGAGATCCTCGACGCCACCACGATGGAGGCCG
CGATCGCGTTCGCCGAGACCGGTCACCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACGATCGAGCGGATC
CTCAACTTCTTCAACGAGGCCGCGCACAAGAACGTGCTGATGAACCTCGCGCTCAACCTCAAGGCGGTGGTCAGCCAGCG
CCTGGTGGTCGGCCGGGACGGTCGGCGCATGCCGGCGGCGGAAGTGCTGATCAACACGCCGATGATCCGCGACCTCTTGC
GCCGCGGTCAGGTGCACGAGATCAAGCAGGCGATGGAGGAGTCGCTGGAGGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGCCTGTACAAGGAAGGCCGCATCGAGATGGAGGAGGCGCTGAAGGCGGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGGCTCTCCCAAGGCGGCGACGTGCACGATCCCTACGCCGACGTGTACTCGCACGGCGAGGAAATGCTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.404

95.699

0.492
  pilU Acinetobacter baylyi ADP1

49.435

95.161

0.47
  pilU Vibrio cholerae strain A1552

48.034

95.699

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.608

91.129

0.425
  pilT Pseudomonas aeruginosa PAK

41.716

90.86

0.379
  pilT Acinetobacter baylyi ADP1

40.588

91.398

0.371
  pilT Acinetobacter baumannii strain A118

40.828

90.86

0.371
  pilT Acinetobacter baumannii D1279779

40.828

90.86

0.371
  pilT Acinetobacter nosocomialis M2

40.828

90.86

0.371
  pilT Pseudomonas stutzeri DSM 10701

40.828

90.86

0.371
  pilT Legionella pneumophila strain Lp02

40.828

90.86

0.371
  pilT Legionella pneumophila strain ERS1305867

40.828

90.86

0.371
  pilT Neisseria gonorrhoeae MS11

39.193

93.28

0.366
  pilT Neisseria meningitidis 8013

39.193

93.28

0.366

Multiple sequence alignment