Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EPZ47_RS25295 Genome accession   NZ_CP035088
Coordinates   5704968..5705393 (-) Length   141 a.a.
NCBI ID   WP_135847212.1    Uniprot ID   -
Organism   Pseudomonas viciae strain 11K1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5699968..5710393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPZ47_RS25280 (EPZ47_25275) - 5700725..5703658 (+) 2934 WP_135847209.1 DUF748 domain-containing protein -
  EPZ47_RS25285 (EPZ47_25280) - 5703660..5703956 (+) 297 WP_135847210.1 DUF2845 domain-containing protein -
  EPZ47_RS25290 (EPZ47_25285) - 5704268..5704621 (-) 354 WP_135847211.1 BON domain-containing protein -
  EPZ47_RS25295 (EPZ47_25290) pilA 5704968..5705393 (-) 426 WP_135847212.1 pilin Machinery gene
  EPZ47_RS25300 (EPZ47_25295) pilB 5705618..5707318 (+) 1701 WP_135847213.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EPZ47_RS25305 (EPZ47_25300) pilC 5707321..5708538 (+) 1218 WP_135847214.1 type II secretion system F family protein Machinery gene
  EPZ47_RS25310 (EPZ47_25305) pilD 5708540..5709409 (+) 870 WP_135847215.1 prepilin peptidase Machinery gene
  EPZ47_RS25315 (EPZ47_25310) coaE 5709421..5710044 (+) 624 WP_135847216.1 dephospho-CoA kinase -
  EPZ47_RS25320 (EPZ47_25315) yacG 5710041..5710247 (+) 207 WP_135847217.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14082.18 Da        Isoelectric Point: 8.1045

>NTDB_id=334337 EPZ47_RS25295 WP_135847212.1 5704968..5705393(-) (pilA) [Pseudomonas viciae strain 11K1]
MNKQKGFTLIELLIVVAIIGILATFALPQYSKYQARAKATAGLAEISALKVPYEDAMNQGTDPSVANMNITSPTSNCTIT
ITGTAAAGAGTIVCTLTNAPAAVAGKTITLSRTAAGAWTCGSNAAQEYLPAGCTGAAAAAG

Nucleotide


Download         Length: 426 bp        

>NTDB_id=334337 EPZ47_RS25295 WP_135847212.1 5704968..5705393(-) (pilA) [Pseudomonas viciae strain 11K1]
ATGAACAAGCAGAAGGGTTTTACGCTGATCGAGCTGCTGATCGTCGTGGCGATCATCGGCATTCTGGCGACGTTCGCGTT
GCCGCAGTATTCCAAGTACCAGGCGCGGGCCAAGGCCACCGCCGGGTTGGCGGAGATTTCTGCGTTGAAGGTGCCGTATG
AGGACGCCATGAATCAAGGTACCGATCCCTCTGTGGCTAACATGAACATAACCTCGCCTACCAGTAACTGCACAATTACG
ATCACAGGTACCGCCGCAGCGGGTGCAGGCACTATCGTCTGTACCCTCACCAACGCCCCTGCTGCCGTAGCCGGTAAGAC
GATCACTCTTTCTCGCACAGCTGCGGGTGCCTGGACGTGCGGTTCCAATGCCGCTCAAGAGTATCTGCCCGCCGGCTGTA
CCGGTGCTGCTGCCGCTGCTGGTTAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-134)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

46.897

100

0.482
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.445

97.163

0.461
  pilA Ralstonia pseudosolanacearum GMI1000

38.75

100

0.44
  pilA2 Legionella pneumophila str. Paris

42.069

100

0.433
  pilA Pseudomonas aeruginosa PAK

40.397

100

0.433
  pilA2 Legionella pneumophila strain ERS1305867

41.379

100

0.426
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.444

95.745

0.426
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.726

100

0.411
  pilA Vibrio cholerae C6706

39.726

100

0.411
  pilA Vibrio cholerae strain A1552

39.726

100

0.411
  pilA/pilA1 Eikenella corrodens VA1

38

100

0.404
  comP Acinetobacter baylyi ADP1

38.255

100

0.404

Multiple sequence alignment