Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EPZ47_RS25305 Genome accession   NZ_CP035088
Coordinates   5707321..5708538 (+) Length   405 a.a.
NCBI ID   WP_135847214.1    Uniprot ID   -
Organism   Pseudomonas viciae strain 11K1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5702321..5713538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPZ47_RS25285 (EPZ47_25280) - 5703660..5703956 (+) 297 WP_135847210.1 DUF2845 domain-containing protein -
  EPZ47_RS25290 (EPZ47_25285) - 5704268..5704621 (-) 354 WP_135847211.1 BON domain-containing protein -
  EPZ47_RS25295 (EPZ47_25290) pilA 5704968..5705393 (-) 426 WP_135847212.1 pilin Machinery gene
  EPZ47_RS25300 (EPZ47_25295) pilB 5705618..5707318 (+) 1701 WP_135847213.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EPZ47_RS25305 (EPZ47_25300) pilC 5707321..5708538 (+) 1218 WP_135847214.1 type II secretion system F family protein Machinery gene
  EPZ47_RS25310 (EPZ47_25305) pilD 5708540..5709409 (+) 870 WP_135847215.1 prepilin peptidase Machinery gene
  EPZ47_RS25315 (EPZ47_25310) coaE 5709421..5710044 (+) 624 WP_135847216.1 dephospho-CoA kinase -
  EPZ47_RS25320 (EPZ47_25315) yacG 5710041..5710247 (+) 207 WP_135847217.1 DNA gyrase inhibitor YacG -
  EPZ47_RS25325 (EPZ47_25320) - 5710244..5710459 (-) 216 WP_003185533.1 hypothetical protein -
  EPZ47_RS25330 (EPZ47_25325) - 5710523..5711209 (-) 687 WP_135847218.1 energy-coupling factor ABC transporter permease -
  EPZ47_RS25335 (EPZ47_25330) - 5711284..5711697 (-) 414 WP_135847219.1 tautomerase family protein -
  EPZ47_RS25340 (EPZ47_25335) - 5711849..5712742 (+) 894 WP_135847220.1 LysR substrate-binding domain-containing protein -
  EPZ47_RS25345 (EPZ47_25340) - 5712811..5713437 (+) 627 WP_003205682.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44281.95 Da        Isoelectric Point: 9.8849

>NTDB_id=334339 EPZ47_RS25305 WP_135847214.1 5707321..5708538(+) (pilC) [Pseudomonas viciae strain 11K1]
MAVKAVKTDVYTWEGKDRKGTKMTGELTGQSPALIKAQLRKQGINPEKVRKKSTSIFSKGKRIKPLDIALFTRQMATMLK
AGVPLLQAFDIIGEGFDNPNMRKLVDEVKQEVAAGNSFAASLRKCPQYFDDLYCNLVDAGEQAGALDTLLDRVATYKEKS
EALKAKIKKAMTYPAAVVLVAAVVTGILLVKVVPQFESVFSGFGAQLPAFTVMVIGLSEFMQQWWWVVLGGLVGAFFGVK
YALRRSEAFRDWRDKWLLKLPLIGTLMYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKRAVQRIRQDVSTGM
QLNFSMRASGIFPNLAIQMTAIGEESGALDDMLDKVASFYEAEVDNLVDNLTSLMEPFIMVVLGVVVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=334339 EPZ47_RS25305 WP_135847214.1 5707321..5708538(+) (pilC) [Pseudomonas viciae strain 11K1]
ATGGCGGTCAAAGCAGTCAAAACCGATGTCTACACGTGGGAAGGCAAAGACCGCAAAGGCACAAAAATGACCGGCGAACT
GACCGGTCAGAGCCCGGCCTTGATCAAGGCTCAGTTGCGTAAACAGGGCATCAACCCGGAGAAGGTGCGCAAGAAGTCCA
CCTCGATATTCAGCAAGGGCAAGCGCATCAAGCCGTTGGATATCGCCCTCTTCACCCGCCAGATGGCCACGATGCTCAAG
GCCGGCGTGCCTCTGTTGCAGGCGTTCGACATCATTGGCGAAGGCTTCGACAACCCCAACATGCGCAAGCTGGTGGACGA
GGTGAAACAGGAAGTCGCAGCCGGCAACAGCTTCGCCGCATCGTTACGCAAGTGCCCGCAATATTTCGACGACCTGTACT
GCAACCTGGTGGACGCCGGTGAACAGGCTGGCGCCCTGGACACGCTGCTGGATCGCGTTGCGACCTACAAGGAAAAGAGC
GAAGCGCTCAAGGCCAAGATCAAGAAAGCCATGACCTATCCAGCGGCCGTGGTCCTCGTCGCTGCGGTGGTCACGGGGAT
CCTGCTGGTCAAGGTCGTGCCGCAGTTCGAATCGGTATTCTCCGGATTCGGCGCACAGCTGCCAGCCTTCACGGTGATGG
TCATCGGTCTGTCGGAATTCATGCAGCAATGGTGGTGGGTGGTGCTCGGCGGGCTGGTGGGGGCGTTTTTTGGCGTGAAA
TACGCCCTCAGGCGCTCCGAGGCCTTTCGCGACTGGCGCGATAAATGGCTGCTCAAGCTGCCACTGATCGGCACTTTGAT
GTACAAATCCGCCGTGGCCCGCTACGCCCGCACGCTGTCCACCACCTTCGCGGCCGGCGTGCCGTTGGTGGAGGCACTGG
ATTCGGTGTCCGGCGCCACCGGCAACGTCGTGTTCAAACGCGCCGTGCAACGCATCCGCCAGGATGTCTCGACCGGCATG
CAGTTGAATTTCTCCATGCGCGCTTCAGGTATCTTTCCGAACCTGGCGATCCAGATGACCGCCATCGGCGAAGAATCCGG
TGCGCTGGACGACATGCTCGACAAGGTGGCGAGTTTTTATGAGGCCGAAGTGGACAATCTGGTGGACAACCTCACCAGTC
TGATGGAACCCTTCATCATGGTGGTCCTGGGGGTGGTCGTCGGTGGCCTGGTGGTTGCCATGTACCTGCCCATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

75.309

100

0.753
  pilC Acinetobacter baylyi ADP1

59.548

98.272

0.585
  pilC Acinetobacter baumannii D1279779

57.843

100

0.583
  pilC Legionella pneumophila strain ERS1305867

54.798

97.778

0.536
  pilG Neisseria gonorrhoeae MS11

43.284

99.259

0.43
  pilC Vibrio campbellii strain DS40M4

43.434

97.778

0.425
  pilG Neisseria meningitidis 44/76-A

42.289

99.259

0.42
  pilC Vibrio cholerae strain A1552

42.677

97.778

0.417
  pilC Thermus thermophilus HB27

36.658

99.012

0.363

Multiple sequence alignment