Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EPZ47_RS25300 Genome accession   NZ_CP035088
Coordinates   5705618..5707318 (+) Length   566 a.a.
NCBI ID   WP_135847213.1    Uniprot ID   -
Organism   Pseudomonas viciae strain 11K1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5700618..5712318
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPZ47_RS25280 (EPZ47_25275) - 5700725..5703658 (+) 2934 WP_135847209.1 DUF748 domain-containing protein -
  EPZ47_RS25285 (EPZ47_25280) - 5703660..5703956 (+) 297 WP_135847210.1 DUF2845 domain-containing protein -
  EPZ47_RS25290 (EPZ47_25285) - 5704268..5704621 (-) 354 WP_135847211.1 BON domain-containing protein -
  EPZ47_RS25295 (EPZ47_25290) pilA 5704968..5705393 (-) 426 WP_135847212.1 pilin Machinery gene
  EPZ47_RS25300 (EPZ47_25295) pilB 5705618..5707318 (+) 1701 WP_135847213.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EPZ47_RS25305 (EPZ47_25300) pilC 5707321..5708538 (+) 1218 WP_135847214.1 type II secretion system F family protein Machinery gene
  EPZ47_RS25310 (EPZ47_25305) pilD 5708540..5709409 (+) 870 WP_135847215.1 prepilin peptidase Machinery gene
  EPZ47_RS25315 (EPZ47_25310) coaE 5709421..5710044 (+) 624 WP_135847216.1 dephospho-CoA kinase -
  EPZ47_RS25320 (EPZ47_25315) yacG 5710041..5710247 (+) 207 WP_135847217.1 DNA gyrase inhibitor YacG -
  EPZ47_RS25325 (EPZ47_25320) - 5710244..5710459 (-) 216 WP_003185533.1 hypothetical protein -
  EPZ47_RS25330 (EPZ47_25325) - 5710523..5711209 (-) 687 WP_135847218.1 energy-coupling factor ABC transporter permease -
  EPZ47_RS25335 (EPZ47_25330) - 5711284..5711697 (-) 414 WP_135847219.1 tautomerase family protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62853.93 Da        Isoelectric Point: 5.5527

>NTDB_id=334338 EPZ47_RS25300 WP_135847213.1 5705618..5707318(+) (pilB) [Pseudomonas viciae strain 11K1]
MNDIALSGLTKQLVLAELITEQNAQQAYQQAQRSRMPLISYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKDTQPSGL
VSEKLVRQHHALPLWRRGNKLYVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSSTGLEGMGDVDLDG
LDIESIDEHKQDSIAGQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGMLREVARPPIQLATRIAARL
KVMASLDISERRKPQDGRLKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCNHCKKAIEIPEETLLKEGFPR
ERIGTFTIYEPVGCEQCNHGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKVMQGMTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=334338 EPZ47_RS25300 WP_135847213.1 5705618..5707318(+) (pilB) [Pseudomonas viciae strain 11K1]
ATGAATGACATCGCCCTCAGCGGCCTGACCAAGCAATTGGTATTGGCCGAACTGATCACCGAGCAAAATGCGCAACAGGC
GTACCAACAGGCCCAGCGCAGTCGCATGCCCCTGATCAGTTATCTGGTGCAAAACAAACTGGTCCAGAGCCGCCAGGTTG
CGGAAATCGCTTCGGAACATTTCGGCGTCGCCCTGCTGGACCTCAACAGCCTGGACAAAGACACCCAACCCAGCGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCATCACGCCCTGCCGTTGTGGCGACGCGGCAACAAGCTGTATGTGGGCATTTCCGA
CCCGACCAATCACCAAGCCATCAATGACATCCAGTTCAGCACCGGCCTGACCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGTGACGCCATCGAGAAGTTTTTCGAGTCCAGCAGCACGGGCCTGGAAGGCATGGGCGACGTCGACCTCGACGGC
CTGGACATCGAGTCGATCGATGAACACAAGCAGGACTCCATCGCCGGCCAGGACACCGACGATGCGCCAGTGGTGCGCTT
CGTCAACAAGATGCTGCTGGACGCGATCAAGGGCGGCTCTTCCGACCTGCACTTCGAACCCTATGAAAAAATTTACCGGG
TGCGGGTGCGTACCGATGGCATGTTGCGGGAAGTGGCCCGGCCTCCGATCCAGTTGGCCACCCGTATCGCCGCACGCCTC
AAGGTGATGGCCAGCCTCGATATCTCCGAACGACGAAAACCCCAGGACGGACGCCTGAAAATGCGCCTGTCGAAAAGCAA
ATCCATCGACTTCCGAGTCAACACCCTGCCCACCCTCTGGGGCGAAAAAGTGGTGATCCGGATACTCGACCCGTCCAGTG
CGCAAATGGGTATCGATGCCCTGGGCTACGAACCGGATCAGAAAGACCTGTACATGGCCGCCCTCAAACAACCACAAGGG
CTGATCCTGGTGACCGGGCCGACCGGCTCGGGCAAGACCGTGTCGCTCTACACCGGGCTGAATATCCTCAACACCGTGGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAATGTGAACCCGCGCCAGG
GACTGGATTTTGCCCAGGCCCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAAATCGCCATCAAGGCCGCCCAGACCGGGCACTTGGTACTCTCGACACTGCACACCAACAGCGCCGCCGA
AACCCTGATCCGCCTGCAGAACATGGGCATTCCGGGCTTCAATATCGCCACGGCGGTGCACCTGATCATCGCCCAGCGGC
TGGCGCGCAAGCTGTGCAACCACTGCAAGAAAGCCATCGAGATCCCCGAGGAGACCTTGCTCAAAGAAGGTTTCCCTCGG
GAACGCATCGGCACATTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAACCACGGCTACAAAGGGCGCGTGGGGGT
TTACGAAGTGGTCAAGAATACGCCTGAGCTGCAACGGCTGATCATGGCCGAGGGCAACTCGCTGGAAATCGACCTGCAAA
TGCGCAAGGACGGTTTCAATGACCTGCGCACATCGGGGCTGCTCAAGGTGATGCAGGGCATGACCAGCCTCGAGGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.657

100

0.587
  pilB Acinetobacter baumannii D1279779

57.27

99.647

0.571
  pilB Legionella pneumophila strain ERS1305867

55.556

100

0.557
  pilB Vibrio cholerae strain A1552

50.442

99.823

0.504
  pilB Vibrio campbellii strain DS40M4

48.582

99.647

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

49.273

97.173

0.479
  pilF Neisseria gonorrhoeae MS11

48.135

99.47

0.479

Multiple sequence alignment