Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ELB75_RS02060 Genome accession   NZ_CP034670
Coordinates   411686..412126 (+) Length   146 a.a.
NCBI ID   WP_126984177.1    Uniprot ID   A0A3S9SMS9
Organism   Eikenella corrodens strain KCOM 3110     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 406686..417126
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ELB75_RS02035 (ELB75_02035) - 407366..407761 (-) 396 WP_126982523.1 energy transducer TonB -
  ELB75_RS02040 (ELB75_02040) orn 407947..408516 (-) 570 WP_126982524.1 oligoribonuclease -
  ELB75_RS02045 (ELB75_02045) - 408646..409284 (+) 639 WP_126982525.1 MBL fold metallo-hydrolase -
  ELB75_RS02050 (ELB75_02050) - 409606..410043 (+) 438 WP_126982526.1 MliC family protein -
  ELB75_RS02055 (ELB75_02055) tsaA 410430..411116 (+) 687 WP_126982527.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  ELB75_RS02060 (ELB75_02060) pilE 411686..412126 (+) 441 WP_126984177.1 pilin Machinery gene
  ELB75_RS02065 (ELB75_02065) - 412212..412676 (+) 465 WP_126984178.1 pilin -
  ELB75_RS02070 (ELB75_02070) - 412616..414451 (+) 1836 WP_126982528.1 O-antigen ligase family protein -
  ELB75_RS02075 (ELB75_02075) - 414469..415233 (+) 765 WP_241236098.1 fimb protein -
  ELB75_RS02085 (ELB75_02085) tehB 415916..416779 (+) 864 WP_126984180.1 tellurite resistance methyltransferase TehB -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15224.43 Da        Isoelectric Point: 9.3658

>NTDB_id=332324 ELB75_RS02060 WP_126984177.1 411686..412126(+) (pilE) [Eikenella corrodens strain KCOM 3110]
MKQVQKGFTLIELMIVIAIIGILAAIALPAYQDYVGRTQVSEALELAGGQKGAVTEYHANHGAWPANNSAAGVAAATSIK
SKYVESVTITNGTITAKMQGSGVNADIQSKQVVLSPTLNAGSYDWKCAAASSNPVPAKYLPSSCRR

Nucleotide


Download         Length: 441 bp        

>NTDB_id=332324 ELB75_RS02060 WP_126984177.1 411686..412126(+) (pilE) [Eikenella corrodens strain KCOM 3110]
TTGAAACAAGTACAAAAAGGTTTTACCCTGATCGAGTTGATGATCGTTATCGCTATTATCGGTATTTTGGCTGCTATCGC
CCTGCCGGCTTACCAAGACTACGTTGGCCGCACCCAAGTTTCTGAGGCTTTGGAATTGGCTGGCGGTCAGAAAGGTGCCG
TAACCGAATACCACGCCAACCATGGCGCATGGCCGGCTAACAATTCTGCTGCCGGTGTGGCTGCTGCTACCAGCATCAAG
AGCAAATATGTTGAAAGCGTAACTATTACCAATGGTACCATCACTGCCAAAATGCAAGGCTCTGGCGTAAATGCTGATAT
TCAAAGCAAACAGGTTGTTCTTTCCCCGACCCTGAATGCCGGCTCTTACGACTGGAAATGTGCTGCCGCAAGCTCTAACC
CGGTTCCCGCCAAATACCTGCCTTCTTCTTGCCGCCGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(37-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S9SMS9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

54.088

100

0.589
  pilE Neisseria gonorrhoeae strain FA1090

53.797

100

0.582
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

39.894

100

0.514
  pilA/pilA1 Eikenella corrodens VA1

42.405

100

0.459
  pilA2 Legionella pneumophila str. Paris

46.154

97.945

0.452
  pilA Ralstonia pseudosolanacearum GMI1000

39.759

100

0.452
  pilA2 Legionella pneumophila strain ERS1305867

45.455

97.945

0.445
  comP Acinetobacter baylyi ADP1

41.497

100

0.418
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.497

100

0.418
  pilA Acinetobacter baumannii strain A118

41.379

99.315

0.411
  pilA Haemophilus influenzae 86-028NP

37.671

100

0.377
  pilA Acinetobacter nosocomialis M2

46.957

78.767

0.37
  pilA Pseudomonas aeruginosa PAK

34.615

100

0.37
  pilA/pilAII Pseudomonas stutzeri DSM 10701

35.099

100

0.363

Multiple sequence alignment