Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CDG68_RS07150 Genome accession   NZ_CP033133
Coordinates   907626..908744 (-) Length   372 a.a.
NCBI ID   WP_087552861.1    Uniprot ID   A0A3G2SZW6
Organism   Acinetobacter wuhouensis strain WCHAW010062     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 902626..913744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG68_RS07120 (CDG68_07125) - 903087..903935 (+) 849 WP_087552858.1 class II glutamine amidotransferase -
  CDG68_RS07125 (CDG68_07130) - 904402..904857 (+) 456 WP_068975804.1 bacteriohemerythrin -
  CDG68_RS07130 (CDG68_07135) - 905090..906172 (+) 1083 WP_213071517.1 hypothetical protein -
  CDG68_RS07135 (CDG68_07140) - 906189..906509 (+) 321 WP_068975802.1 RnfH family protein -
  CDG68_RS07140 (CDG68_07145) - 906593..906991 (-) 399 WP_087552860.1 outer membrane protein assembly factor BamE -
  CDG68_RS07145 (CDG68_07150) fur 907104..907541 (+) 438 WP_068975800.1 ferric iron uptake transcriptional regulator -
  CDG68_RS07150 (CDG68_07155) pilU 907626..908744 (-) 1119 WP_087552861.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CDG68_RS07155 (CDG68_07160) pilT 908768..909805 (-) 1038 WP_068975798.1 type IV pilus twitching motility protein PilT Machinery gene
  CDG68_RS07160 (CDG68_07165) - 909929..910621 (+) 693 WP_087552862.1 YggS family pyridoxal phosphate-dependent enzyme -
  CDG68_RS07165 (CDG68_07170) - 911045..911845 (+) 801 WP_087552863.1 thioesterase family protein -
  CDG68_RS07170 (CDG68_07175) - 912036..912857 (+) 822 WP_087552864.1 hypothetical protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41872.27 Da        Isoelectric Point: 6.5074

>NTDB_id=321651 CDG68_RS07150 WP_087552861.1 907626..908744(-) (pilU) [Acinetobacter wuhouensis strain WCHAW010062]
MDFNDLLNMMIEQKASDLFITADVEPSMKINGQIVPVVKTKLNGDIIMQLLNSIMSEKQRKEFAETRECNFAITNKDKTA
RFRVSAFQQRDLPGMVLRRIETKIPTMEELKLPPILKDLAMTKRGIIIFVGATGTGKSTSLASIIGYRNHNSKGHIITIE
DPIEFVHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFESDRHSQLFMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDLIRKGDIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLQIKLDEEGGAQLLNANTKLTFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=321651 CDG68_RS07150 WP_087552861.1 907626..908744(-) (pilU) [Acinetobacter wuhouensis strain WCHAW010062]
ATGGACTTTAATGACTTGCTCAATATGATGATTGAGCAAAAAGCATCCGATTTATTTATTACGGCTGATGTTGAACCATC
AATGAAAATTAATGGTCAAATCGTACCTGTGGTAAAAACCAAGTTGAATGGCGACATCATCATGCAATTGCTCAACTCAA
TTATGAGTGAAAAGCAACGTAAAGAATTTGCCGAAACTCGTGAGTGTAACTTCGCGATTACCAATAAAGACAAGACAGCG
CGTTTTCGTGTCAGCGCATTTCAGCAACGTGATCTGCCAGGCATGGTTTTACGTCGTATTGAAACTAAAATTCCAACGAT
GGAAGAGTTAAAACTTCCACCAATTCTGAAAGATTTAGCCATGACCAAACGTGGCATTATTATCTTTGTAGGTGCGACAG
GTACGGGTAAATCAACCTCTTTAGCATCCATTATTGGCTATCGTAACCACAATTCCAAAGGTCATATCATTACCATTGAA
GACCCAATTGAATTTGTTCATGAACATGCGGGTTGTATTATTACCCAACGCGAAGTCGGTATTGATACCGATTCATTTGA
AATTGCATTGAAAAATACCTTACGGCAAGCCCCTGATGTGATTTTGATTGGTGAGATTCGCTCTCGTGAAACCATGGATT
ACGCGATTGCCTTTGCGGAAACGGGTCACTTGGTTTTTGCTACACTGCATGCCAACAACGCCAACCAAGCCATTGATCGT
ATTATTCACTTCTTTGAATCTGACCGTCATAGTCAATTGTTTATGGATTTGTCGCTCAATTTAAAAGCGATGATCGCGCA
ACAGTTGATTCCAACACCAGATGGTAACTCTCGTCGTGCTGCGATTGAAATTCTAATCAACTCCCCTTTAATTTCTGATT
TGATCCGTAAAGGTGACATTCACGAAATTAAAGATTTGATGAAACGCTCACGTGAGTTAGGGATGCAAACCTTTGACCAA
GCCTTGTTTGACTTATATAAAGCAGGGCAAATCACCTATAAAGATGCACTAAAACATGCAGATTCACCAAATGATTTACG
TCTACAAATCAAACTGGATGAAGAAGGTGGCGCGCAACTGCTCAATGCAAATACTAAATTAACTTTCGACGGTCAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G2SZW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.455

100

0.777
  pilU Pseudomonas stutzeri DSM 10701

67.925

99.731

0.677
  pilU Vibrio cholerae strain A1552

55.342

98.118

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.064

93.011

0.401
  pilT Acinetobacter baumannii strain A118

40.525

92.204

0.374
  pilT Acinetobacter baumannii D1279779

40.525

92.204

0.374
  pilT Acinetobacter nosocomialis M2

40.525

92.204

0.374
  pilT Pseudomonas aeruginosa PAK

40.173

93.011

0.374
  pilT Legionella pneumophila strain ERS1305867

41.071

90.323

0.371
  pilT Legionella pneumophila strain Lp02

41.071

90.323

0.371
  pilT Acinetobacter baylyi ADP1

39.942

92.204

0.368
  pilT Pseudomonas stutzeri DSM 10701

39.306

93.011

0.366
  pilT Neisseria meningitidis 8013

39.017

93.011

0.363
  pilT Neisseria gonorrhoeae MS11

39.017

93.011

0.363

Multiple sequence alignment