Detailed information NTG0313853

Bioinformatically predicted

Overview

• Name: pilA/pilAI
• Type: Machinery gene
• Locus tag: CDG61_RS01950
• Genome accession: NZ_CP032143
• Coordinates: 334227..334649 (-)
• Length: 140 a.a.
• NCBI ID: WP_416240703.1
• Uniprot ID: -
• Organism: Acinetobacter sp. WCHAc010052
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	321628..333308	334227..334649	flank	919

Gene organization within MGE regions

Location: 321628..334649

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CDG61_RS01905 (CDG61_01905)	-	321628..322878 (+)	1251	WP_087549852.1	O-antigen translocase	-
CDG61_RS01910 (CDG61_01910)	-	323039..324022 (+)	984	WP_171405564.1	glycosyltransferase	-
CDG61_RS01915 (CDG61_01915)	-	325664..326554 (+)	891	WP_087549854.1	glycosyltransferase family 2 protein	-
CDG61_RS01920 (CDG61_01920)	-	326556..327365 (+)	810	WP_087549855.1	glycosyltransferase	-
CDG61_RS17600 (CDG61_01925)	-	327409..328695 (+)	1287	WP_087549856.1	sugar transferase	-
CDG61_RS01930 (CDG61_01930)	-	328729..330432 (-)	1704	WP_087549857.1	PglL family O-oligosaccharyltransferase	-
CDG61_RS01935 (CDG61_01935)	tfpZ	330473..331207 (-)	735	WP_087549858.1	TfpX/TfpZ family type IV pilin accessory protein	-
CDG61_RS01945 (CDG61_01945)	-	332886..333308 (+)	423	WP_087549860.1	pilin	-
CDG61_RS01950 (CDG61_01950)	pilA/pilAI	334227..334649 (-)	423	WP_416240703.1	pilin	Machinery gene

Sequence

Protein

• Length: 140 a.a.
• Molecular weight: 14425.41 Da
• Isoelectric Point: 5.0499

>NTDB_id=313853 CDG61_RS01950 WP_416240703.1 334227..334649(-) (pilA/pilAI) [Acinetobacter sp. WCHAc010052]
MQKGFTLIELMIVVAIIGILAAIAIPAYQDYIAKSQATSGLAEINPAKTQFEVAVNEGKDPSTDASAAGYIGVPSVTRYC
EVSTTFASTTGVGTIVCELQNGNATKINGKTITLNRSGEGAWTCATGNGLDAKFKPGSCS

Nucleotide

• Length: 423 bp

>NTDB_id=313853 CDG61_RS01950 WP_416240703.1 334227..334649(-) (pilA/pilAI) [Acinetobacter sp. WCHAc010052]
ATGCAAAAAGGTTTTACCTTAATTGAGCTCATGATTGTGGTAGCAATTATTGGTATTTTGGCAGCTATTGCGATTCCTGC
TTACCAGGACTATATTGCTAAATCACAAGCGACTTCTGGTCTGGCGGAAATTAACCCTGCTAAAACTCAATTCGAAGTTG
CAGTAAATGAAGGTAAAGATCCTTCTACCGATGCTTCTGCTGCAGGTTATATTGGTGTGCCTTCTGTAACTCGTTATTGT
GAAGTTAGTACAACTTTTGCTTCAACTACAGGTGTAGGTACAATTGTTTGTGAATTACAAAATGGTAATGCAACTAAGAT
TAATGGAAAAACCATTACTTTAAATCGTAGTGGTGAAGGTGCGTGGACTTGTGCAACTGGTAATGGCTTAGACGCTAAAT
TTAAACCAGGTTCTTGTTCTTAA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (33-139)

• Prokaryotic N-terminal methylation motif (N_methyl) (2-24)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA/pilAI	Pseudomonas stutzeri DSM 10701	60	100	0.6
pilA/pilAII	Pseudomonas stutzeri DSM 10701	53.285	97.857	0.521
pilA	Acinetobacter baumannii strain A118	52.518	99.286	0.521
pilA2	Legionella pneumophila strain ERS1305867	48.921	99.286	0.486
pilA2	Legionella pneumophila str. Paris	48.201	99.286	0.479
comP	Acinetobacter baylyi ADP1	41.83	100	0.457
pilA	Pseudomonas aeruginosa PAK	40.909	100	0.45
pilA/pilA1	Eikenella corrodens VA1	40.523	100	0.443
pilA	Ralstonia pseudosolanacearum GMI1000	36.527	100	0.436
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	42.361	100	0.436
pilA	Vibrio cholerae C6706	42.361	100	0.436
pilA	Vibrio cholerae strain A1552	42.361	100	0.436
pilE	Neisseria gonorrhoeae strain FA1090	35.625	100	0.407
pilE	Neisseria gonorrhoeae MS11	34.177	100	0.386