Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D1367_RS13935 Genome accession   NZ_CP031941
Coordinates   3167367..3168494 (-) Length   375 a.a.
NCBI ID   WP_118166981.1    Uniprot ID   -
Organism   Nostoc sphaeroides strain Kutzing En     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3162367..3173494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1367_RS13905 - 3162470..3162694 (-) 225 WP_118166975.1 DUF433 domain-containing protein -
  D1367_RS13910 - 3162925..3163248 (-) 324 WP_244945005.1 type II toxin-antitoxin system PemK/MazF family toxin -
  D1367_RS13915 - 3163242..3163502 (-) 261 WP_118166977.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  D1367_RS13920 bioU 3164027..3165049 (+) 1023 WP_118166978.1 (S)-8-amino-7-oxononanoate synthase BioU -
  D1367_RS13925 - 3165285..3165845 (-) 561 WP_118166979.1 hypothetical protein -
  D1367_RS13930 - 3166028..3167251 (-) 1224 WP_118166980.1 type II secretion system F family protein -
  D1367_RS13935 pilT 3167367..3168494 (-) 1128 WP_118166981.1 type IV pilus twitching motility protein PilT Machinery gene
  D1367_RS13940 pilB/pilB1 3168623..3170626 (-) 2004 WP_118166982.1 GspE/PulE family protein Machinery gene
  D1367_RS13945 grpE 3170995..3171744 (+) 750 WP_118166983.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41023.29 Da        Isoelectric Point: 7.4775

>NTDB_id=312839 D1367_RS13935 WP_118166981.1 3167367..3168494(-) (pilT) [Nostoc sphaeroides strain Kutzing En]
MEMMIEDLMEQLIELGGSDMHLSAGLPPYFRISGKLTPIGEHVLTADQCQRLIFSMLNNTQRKTLEQNWELDCSYGVKGL
ARFRVNVYKERGSYAACLRALSSKIPNFEKLGLPDIVREMTDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIVLVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPHERQTQVRVQLSNSLVAVFSQTLVSKKNPKPGEYGRVMAQEILIVTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADFYKAGTISFEAAMSKTSKPDEIQRLIGTSVPPQAAGGKSGAAAKAH

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=312839 D1367_RS13935 WP_118166981.1 3167367..3168494(-) (pilT) [Nostoc sphaeroides strain Kutzing En]
ATGGAAATGATGATTGAAGACCTGATGGAGCAGTTGATTGAATTGGGTGGCTCGGATATGCATTTATCCGCAGGTTTGCC
TCCCTACTTCCGCATCAGTGGCAAACTCACCCCCATCGGTGAGCATGTATTGACAGCCGATCAATGTCAAAGGCTGATTT
TTAGTATGCTCAACAACACCCAGCGTAAAACCTTAGAGCAGAACTGGGAATTGGATTGTTCTTATGGTGTTAAGGGTTTG
GCTCGCTTCCGGGTCAATGTCTATAAAGAACGTGGTTCTTATGCTGCTTGCTTACGGGCATTAAGTTCTAAGATTCCTAA
CTTTGAAAAATTAGGTTTGCCAGATATCGTGCGGGAAATGACAGATAAGCCCAGAGGGCTAATTCTGGTGACAGGCCCTA
CAGGTTCGGGTAAGACAACTACCCTAGCGGCAATGATTGACTTGATTAACCGCACCAAGGCAGAACATATTTTAACAGTG
GAAGATCCAATTGAATTTGTCTACGAACCAATTAAAAGCTTGGTTCACCAACGACAACTGGGTGAAGATACTAAGAGTTT
TGCTAATGCTTTGAAAGCAGCTTTGCGGGAAGATCCAGATATTGTTCTGGTGGGGGAAATGCGCGATTTAGAAACGATTT
CTTTGGCGATTTCGGCAGCAGAAACAGGACACTTGGTATTTGGTACTCTCCACACTAGTTCTGCTGCACAGACAGTTGAC
CGGATTATCGACGTTTTCCCCCATGAAAGACAAACTCAAGTGCGGGTGCAGTTATCTAACTCATTAGTAGCGGTATTTAG
CCAAACTTTGGTATCCAAGAAAAACCCTAAACCCGGTGAATATGGTCGGGTGATGGCTCAAGAAATTCTGATTGTCACTC
CTGCTATTTCCAACTTAATTCGAGAAGGCAAAACATCTCAAATTTACTCGGCTATTCAAACTGGCGGCAAATTGGGAATG
CAAACTCTGGAGAAGGTTTTAGCCGATTTTTACAAGGCCGGAACGATTTCCTTTGAAGCGGCAATGTCTAAGACTTCTAA
GCCAGATGAAATCCAACGTCTCATCGGTACTTCTGTACCACCGCAGGCAGCAGGTGGGAAATCTGGTGCGGCTGCCAAAG
CACATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.467

92.533

0.504
  pilT Pseudomonas stutzeri DSM 10701

53.868

93.067

0.501
  pilT Acinetobacter baylyi ADP1

52.738

92.533

0.488
  pilT Legionella pneumophila strain Lp02

52.738

92.533

0.488
  pilT Legionella pneumophila strain ERS1305867

52.738

92.533

0.488
  pilT Acinetobacter baumannii D1279779

51.873

92.533

0.48
  pilT Acinetobacter nosocomialis M2

51.873

92.533

0.48
  pilT Acinetobacter baumannii strain A118

51.873

92.533

0.48
  pilT Neisseria gonorrhoeae MS11

50.737

90.4

0.459
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.857

93.333

0.456
  pilT Neisseria meningitidis 8013

50.442

90.4

0.456
  pilT Vibrio cholerae strain A1552

49.415

91.2

0.451
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.415

91.2

0.451

Multiple sequence alignment