Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   D1367_RS13940 Genome accession   NZ_CP031941
Coordinates   3168623..3170626 (-) Length   667 a.a.
NCBI ID   WP_118166982.1    Uniprot ID   A0A5P8W6C0
Organism   Nostoc sphaeroides strain Kutzing En     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3163623..3175626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1367_RS13920 bioU 3164027..3165049 (+) 1023 WP_118166978.1 (S)-8-amino-7-oxononanoate synthase BioU -
  D1367_RS13925 - 3165285..3165845 (-) 561 WP_118166979.1 hypothetical protein -
  D1367_RS13930 - 3166028..3167251 (-) 1224 WP_118166980.1 type II secretion system F family protein -
  D1367_RS13935 pilT 3167367..3168494 (-) 1128 WP_118166981.1 type IV pilus twitching motility protein PilT Machinery gene
  D1367_RS13940 pilB/pilB1 3168623..3170626 (-) 2004 WP_118166982.1 GspE/PulE family protein Machinery gene
  D1367_RS13945 grpE 3170995..3171744 (+) 750 WP_118166983.1 nucleotide exchange factor GrpE -
  D1367_RS13950 dnaK 3171974..3173932 (+) 1959 WP_118166984.1 molecular chaperone DnaK -
  D1367_RS30140 - 3174255..3174521 (-) 267 WP_147337367.1 hypothetical protein -

Sequence

Protein


Download         Length: 667 a.a.        Molecular weight: 74395.13 Da        Isoelectric Point: 5.1210

>NTDB_id=312840 D1367_RS13940 WP_118166982.1 3168623..3170626(-) (pilB/pilB1) [Nostoc sphaeroides strain Kutzing En]
MNSSPQRRSTALTTRTEFSPFGNKLVQSGYVNTEQMRQALIESRKSGRPLTEVLEAITGQQLSPELLRQYKKQQLFELKI
LYGVEFLDPEVNSFGNTEMANLIETLIPVDICRRHRLVPLSKHEDQTPPSVLVAMVAPDNLEASDDLNRILRPQGLALQR
MVITQEDYQQLINQYLDEMAVKQKHLERENSTDISQDLENLGNLDIEDAPEEMEADLGAAMKGAEDAPVINLVNRILAKA
LHEGVSDIHIEPQEENLRIRFRKDGVLREAFPSMPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRLFEGRKVDFRVNT
LPSRYGEKVVLRILDNSSTQLGLDKLITDPETLNIVKDMVSRPFGLILVTGPTGSGKTTSLYSALSEKNDPGINISTVED
PIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGEM
GIEPFMVSSSLIGVLAQRLVRRVCSECRIAYTPTTEELARYGLSASSDVAVSFYKANTLTLDAIAEAKTKNQLCSKCNGV
GYKGRCGVYEVMRVTENLQTLINEDAPTERIKEVAIEEGMKTLLAYSLDLVREGSTTLEEVERVTFTDTGLEAELKAKRK
TGLTCRTCEATLKPEWLDCPYCMTSRF

Nucleotide


Download         Length: 2004 bp        

>NTDB_id=312840 D1367_RS13940 WP_118166982.1 3168623..3170626(-) (pilB/pilB1) [Nostoc sphaeroides strain Kutzing En]
ATGAACTCGTCACCACAACGGCGCAGTACCGCTCTAACTACCAGAACAGAGTTTTCGCCCTTTGGCAACAAGCTAGTGCA
ATCTGGCTATGTCAATACCGAACAGATGAGGCAGGCACTAATTGAAAGCCGCAAATCTGGCAGACCCTTAACGGAAGTAC
TAGAGGCAATCACTGGGCAACAACTATCACCTGAGTTGCTCAGGCAATACAAAAAACAGCAGCTATTTGAACTAAAAATA
CTATACGGTGTTGAATTCCTTGATCCGGAAGTCAACTCCTTTGGCAACACGGAGATGGCGAACCTGATTGAAACCCTCAT
CCCGGTGGATATTTGCCGTCGCCATCGTTTAGTACCACTATCGAAACACGAAGACCAAACCCCGCCCTCAGTTTTAGTGG
CGATGGTGGCTCCAGATAATCTAGAGGCTTCTGATGACCTAAATCGCATCTTGCGCCCCCAAGGCTTGGCCTTGCAGCGC
ATGGTGATTACCCAGGAAGACTACCAACAGCTAATCAACCAATATTTGGATGAAATGGCTGTTAAGCAAAAGCACCTGGA
ACGAGAAAACTCTACAGATATTAGTCAGGATTTAGAAAATCTCGGAAATCTCGATATTGAGGATGCTCCTGAAGAAATGG
AGGCTGATCTAGGAGCAGCCATGAAGGGTGCAGAGGATGCCCCAGTTATCAACCTAGTCAACAGAATCCTGGCTAAAGCC
TTGCATGAGGGCGTTTCTGACATTCATATCGAACCGCAAGAAGAAAACTTACGCATTCGTTTTCGGAAAGATGGCGTACT
GCGTGAAGCTTTCCCCTCAATGCCGAAAAAAATCATTCCGGCGGTGACAGCTCGATTTAAAATCATCTCCAATCTCGACA
TTGCCGAACGTCGTCTACCACAAGATGGACGCATCCGGCGGCTGTTTGAGGGACGTAAGGTGGATTTTCGTGTGAATACC
TTGCCCAGTCGCTACGGGGAAAAGGTGGTGCTGCGGATTTTGGATAACTCCTCTACCCAACTGGGATTAGATAAGTTAAT
TACCGATCCAGAGACTTTGAATATTGTCAAGGATATGGTCAGCCGTCCCTTTGGTCTAATTTTGGTAACTGGGCCAACTG
GTTCTGGAAAAACAACTTCGCTGTATTCTGCATTATCAGAAAAGAATGATCCCGGAATTAATATTAGTACTGTGGAAGAC
CCCATTGAGTACAGTCTTCCAGGGATTACTCAAGTACAGGTGATTCGAGAAAAAGGGCTGGATTTTGCTACCGCTTTGCG
GGCTTTCTTGCGGCAAGATCCAGATGTGCTGCTGGTGGGTGAAACGCGGGATAAGGAAACGGCAAAAACAGCAATTGAAG
CTGCGTTAACTGGTCACTTGGTATTAACTACCTTACATACCAATGATGCCCCAGGTGCGATCGCTCGTTTGGGAGAAATG
GGGATTGAGCCTTTCATGGTTTCTAGTTCCCTAATTGGCGTTTTAGCTCAACGTTTGGTGCGGCGTGTATGTTCTGAATG
TCGGATTGCCTACACTCCCACAACGGAGGAATTGGCTCGTTATGGTCTATCAGCTTCCTCGGATGTCGCAGTTAGCTTCT
ATAAGGCTAACACCTTGACATTAGATGCGATCGCAGAAGCTAAAACCAAAAATCAGCTTTGCTCAAAATGTAATGGGGTC
GGCTACAAAGGGCGTTGTGGTGTTTATGAAGTCATGCGAGTCACCGAAAACCTGCAAACTCTCATCAACGAAGATGCACC
CACAGAACGCATCAAGGAAGTGGCAATAGAAGAGGGCATGAAAACCTTGCTGGCTTACAGTTTGGACTTAGTGCGCGAAG
GTTCCACCACTTTAGAAGAAGTAGAACGAGTCACGTTTACTGATACCGGTTTGGAAGCCGAGTTAAAAGCCAAACGCAAG
ACTGGTCTTACCTGCCGGACTTGCGAGGCCACATTGAAACCAGAATGGCTCGATTGTCCCTACTGTATGACATCTCGGTT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(235-502)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(63-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P8W6C0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.415

99.1

0.658

Multiple sequence alignment